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2lxb

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Current revision

Solution structure of the Sgt2 homodimerization domain

Structural highlights

2lxb is a 2 chain structure with sequence from Saccharomyces cerevisiae S288C. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SGT2_YEAST Co-chaperone that binds to the molecular chaperone Hsp70 (SSA1 and SSA2). Regulates Hsp70 ATPase activity (By similarity). Required for recovery from heat shock.^[1]

Publication Abstract from PubMed

In the cytoplasm, the correct delivery of membrane proteins is an essential and highly regulated process. The posttranslational targeting of the important tail-anchor membrane (TA) proteins has recently been under intense investigation. A specialized pathway, called the guided entry of TA proteins (GET) pathway in yeast and the transmembrane domain recognition complex (TRC) pathway in vertebrates, recognizes endoplasmic-reticulum-targeted TA proteins and delivers them through a complex series of handoffs. An early step is the formation of a complex between Sgt2/SGTA, a cochaperone with a presumed ubiquitin-like-binding domain (UBD), and Get5/UBL4A, a ubiquitin-like domain (UBL)-containing protein. We structurally characterize this UBD/UBL interaction for both yeast and human proteins. This characterization is supported by biophysical studies that demonstrate that complex formation is mediated by electrostatics, generating an interface that has high-affinity with rapid kinetics. In total, this work provides a refined model of the interplay of Sgt2 homologs in TA targeting.

Structures of the Sgt2/SGTA Dimerization Domain with the Get5/UBL4A UBL Domain Reveal an Interaction that Forms a Conserved Dynamic Interface.,Chartron JW, Vandervelde DG, Clemons WM Jr Cell Rep. 2012 Nov 7. pii: S2211-1247(12)00346-4. doi:, 10.1016/j.celrep.2012.10.010. PMID:23142665^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Angeletti PC, Walker D, Panganiban AT. Small glutamine-rich protein/viral protein U-binding protein is a novel cochaperone that affects heat shock protein 70 activity. Cell Stress Chaperones. 2002 Jul;7(3):258-68. PMID:12482202
↑ Chartron JW, Vandervelde DG, Clemons WM Jr. Structures of the Sgt2/SGTA Dimerization Domain with the Get5/UBL4A UBL Domain Reveal an Interaction that Forms a Conserved Dynamic Interface. Cell Rep. 2012 Nov 7. pii: S2211-1247(12)00346-4. doi:, 10.1016/j.celrep.2012.10.010. PMID:23142665 doi:http://dx.doi.org/10.1016/j.celrep.2012.10.010

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lxb"

Categories: Large Structures | Saccharomyces cerevisiae S288C | Chartron JW | Clemons Jr WM | Vandervelde DG

@@ Line 1: / Line 1: @@
 ==Solution structure of the Sgt2 homodimerization domain==
-<StructureSection load='2lxb' size='340' side='right' caption='[[2lxb]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+<StructureSection load='2lxb' size='340' side='right'caption='[[2lxb]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2lxb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LXB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LXB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2lxb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LXB FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4goc|4goc]], [[2lxa|2lxa]], [[2lxc|2lxc]], [[4god|4god]], [[4goe|4goe]], [[4gof|4gof]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SGT2, UNF346, YOR007C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lxb OCA], [https://pdbe.org/2lxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lxb RCSB], [https://www.ebi.ac.uk/pdbsum/2lxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lxb ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lxb OCA], [http://pdbe.org/2lxb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lxb RCSB], [http://www.ebi.ac.uk/pdbsum/2lxb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lxb ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SGT2_YEAST SGT2_YEAST]] Co-chaperone that binds to the molecular chaperone Hsp70 (SSA1 and SSA2). Regulates Hsp70 ATPase activity (By similarity). Required for recovery from heat shock.<ref>PMID:12482202</ref>
+[https://www.uniprot.org/uniprot/SGT2_YEAST SGT2_YEAST] Co-chaperone that binds to the molecular chaperone Hsp70 (SSA1 and SSA2). Regulates Hsp70 ATPase activity (By similarity). Required for recovery from heat shock.<ref>PMID:12482202</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 18: @@
 </div>
 <div class="pdbe-citations 2lxb" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Small glutamine-rich tetratricopeptide repeat containing protein alpha|Small glutamine-rich tetratricopeptide repeat containing protein alpha]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Baker's yeast]]
+[[Category: Large Structures]]
-[[Category: Chartron, J W]]
+[[Category: Saccharomyces cerevisiae S288C]]
-[[Category: Clemons, W M]]
+[[Category: Chartron JW]]
-[[Category: Vandervelde, D G]]
+[[Category: Clemons Jr WM]]
-[[Category: Four-helix bundle]]
+[[Category: Vandervelde DG]]
-[[Category: Get pathway]]
-[[Category: Get5 binding domain]]
-[[Category: Protein binding]]
-[[Category: Protein-protein interaction]]

2lxb

From Proteopedia

Current revision

Solution structure of the Sgt2 homodimerization domain

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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