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| | ==Crystal Structure of LC3-p62 complex== | | ==Crystal Structure of LC3-p62 complex== |
| - | <StructureSection load='2zjd' size='340' side='right' caption='[[2zjd]], [[Resolution|resolution]] 1.56Å' scene=''> | + | <StructureSection load='2zjd' size='340' side='right'caption='[[2zjd]], [[Resolution|resolution]] 1.56Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2zjd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZJD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZJD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2zjd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZJD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZJD FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zjd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zjd OCA], [http://pdbe.org/2zjd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zjd RCSB], [http://www.ebi.ac.uk/pdbsum/2zjd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zjd ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zjd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zjd OCA], [https://pdbe.org/2zjd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zjd RCSB], [https://www.ebi.ac.uk/pdbsum/2zjd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zjd ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN]] Involved in formation of autophagosomal vacuoles (autophagosomes). [[http://www.uniprot.org/uniprot/SQSTM_MOUSE SQSTM_MOUSE]] Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures). Links ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Adapter that mediates the interaction between TRAF6 and CYLD.<ref>PMID:14960283</ref> <ref>PMID:18382763</ref> | + | [https://www.uniprot.org/uniprot/MLP3B_HUMAN MLP3B_HUMAN] Involved in formation of autophagosomal vacuoles (autophagosomes). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | ==See Also== | | ==See Also== |
| | *[[Microtubule-associated protein|Microtubule-associated protein]] | | *[[Microtubule-associated protein|Microtubule-associated protein]] |
| | + | *[[Microtubule-associated protein 3D structures|Microtubule-associated protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Ezaki, J]] | + | [[Category: Large Structures]] |
| - | [[Category: Ichimura, Y]] | + | [[Category: Mus musculus]] |
| - | [[Category: Komatsu, M]] | + | [[Category: Ezaki J]] |
| - | [[Category: Kominami, E]] | + | [[Category: Ichimura Y]] |
| - | [[Category: Kumanomidou, T]] | + | [[Category: Komatsu M]] |
| - | [[Category: Mizushima, T]] | + | [[Category: Kominami E]] |
| - | [[Category: Sou, Y]] | + | [[Category: Kumanomidou T]] |
| - | [[Category: Tanaka, K]] | + | [[Category: Mizushima T]] |
| - | [[Category: Ueno, T]] | + | [[Category: Sou Y]] |
| - | [[Category: Yamane, T]] | + | [[Category: Tanaka K]] |
| - | [[Category: Alternative splicing]]
| + | [[Category: Ueno T]] |
| - | [[Category: Apoptosis]]
| + | [[Category: Yamane T]] |
| - | [[Category: Apoptosis inhibitor-apoptosis complex]]
| + | |
| - | [[Category: Autophagy]]
| + | |
| - | [[Category: Cytoplasm]]
| + | |
| - | [[Category: Cytoplasmic vesicle]]
| + | |
| - | [[Category: Differentiation]]
| + | |
| - | [[Category: Endosome]]
| + | |
| - | [[Category: Immune response]]
| + | |
| - | [[Category: Lc3]]
| + | |
| - | [[Category: Lipoprotein]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Metal-binding]]
| + | |
| - | [[Category: Microtubule-associated protein 1 light chain 3]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: P62]]
| + | |
| - | [[Category: Phosphoprotein]]
| + | |
| - | [[Category: Ubl conjugation pathway]]
| + | |
| - | [[Category: Zinc]]
| + | |
| - | [[Category: Zinc-finger]]
| + | |
| Structural highlights
Function
MLP3B_HUMAN Involved in formation of autophagosomal vacuoles (autophagosomes).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Impairment of autophagic degradation of the ubiquitin- and LC3-binding protein "p62" leads to the formation of cytoplasmic inclusion bodies. However, little is known about the sorting mechanism of p62 to autophagic degradation. Here we identified a motif of murine p62 consisting of 11 amino acids (Ser334-Ser344) containing conserved acidic and hydrophobic residues across species, as an LC3 recognition sequence (LRS). The crystal structure of the LC3-LRS complex at 1.56 angstroms resolution revealed interaction of Trp340 and Leu343 of p62 with different hydrophobic pockets on the ubiquitin fold of LC3. In vivo analyses demonstrated that p62 mutants lacking LC3 binding ability accumulated without entrapping into autophagosomes in the cytoplasm and subsequently formed ubiquitin-positive inclusion bodies as in autophagy-deficient cells. These results demonstrate that the intracellular level of p62 is tightly regulated by autophagy through the direct interaction of LC3 with p62 and reveal that selective turnover of p62 via autophagy controls inclusion body formation.
Structural basis for sorting mechanism of p62 in selective autophagy.,Ichimura Y, Kumanomidou T, Sou YS, Mizushima T, Ezaki J, Ueno T, Kominami E, Yamane T, Tanaka K, Komatsu M J Biol Chem. 2008 Aug 15;283(33):22847-57. Epub 2008 Jun 4. PMID:18524774[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ichimura Y, Kumanomidou T, Sou YS, Mizushima T, Ezaki J, Ueno T, Kominami E, Yamane T, Tanaka K, Komatsu M. Structural basis for sorting mechanism of p62 in selective autophagy. J Biol Chem. 2008 Aug 15;283(33):22847-57. Epub 2008 Jun 4. PMID:18524774 doi:http://dx.doi.org/M802182200
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