3bw8

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(New page: 200px {{Structure |PDB= 3bw8 |SIZE=350|CAPTION= <scene name='initialview01'>3bw8</scene>, resolution 1.80&Aring; |SITE= <scene name='pdbsite=AC1:So4+Binding+Site+...)
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[[Image:3bw8.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of the Clostridium limosum C3 exoenzyme==
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|PDB= 3bw8 |SIZE=350|CAPTION= <scene name='initialview01'>3bw8</scene>, resolution 1.80&Aring;
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<StructureSection load='3bw8' size='340' side='right'caption='[[3bw8]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+206'>AC1</scene>
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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<table><tr><td colspan='2'>[[3bw8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hathewaya_limosa Hathewaya limosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BW8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BW8 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE= c3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1536 Clostridium limosum])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00233 VIP2]</span>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bw8 OCA], [https://pdbe.org/3bw8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bw8 RCSB], [https://www.ebi.ac.uk/pdbsum/3bw8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bw8 ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1ojq|1OJQ]], [[1ojz|1OJZ]], [[1gzf|1GZF]], [[1gze|1GZE]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bw8 OCA], [http://www.ebi.ac.uk/pdbsum/3bw8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bw8 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/ARC3_HATLI ARC3_HATLI] ADP-ribosylates eukaryotic Rho and Rac proteins on an asparagine residue.[UniProtKB:P15879]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bw8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bw8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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C3-like toxins ADP-ribosylate and inactivate Rho GTPases. Seven C3-like ADP-ribosyltransferases produced by Clostridium botulinum, Clostridium limosum, Bacillus cereus and Staphylococcus aureus were identified and two representatives--C3bot from C. botulinum and C3stau2 from S. aureus--were crystallized. Here we present the 1.8A structure of C. limosum C3 transferase C3lim and compare it to the structures of other family members. In contrast to the structure of apo-C3bot, the canonical ADP-ribosylating turn turn motif is observed in a primed conformation, ready for NAD binding. This suggests an impact on the binding mode of NAD and on the transferase reaction. The crystal structure explains why auto-ADP-ribosylation of C3lim at Arg41 interferes with the ADP-ribosyltransferase activity of the toxin.
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'''Crystal structure of the Clostridium limosum C3 exoenzyme'''
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Crystal structure of the Clostridium limosum C3 exoenzyme.,Vogelsgesang M, Stieglitz B, Herrmann C, Pautsch A, Aktories K FEBS Lett. 2008 Apr 2;582(7):1032-6. Epub 2008 Mar 4. PMID:18325337<ref>PMID:18325337</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3bw8" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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C3-like toxins ADP-ribosylate and inactivate Rho GTPases. Seven C3-like ADP-ribosyltransferases produced by Clostridium botulinum, Clostridium limosum, Bacillus cereus and Staphylococcus aureus were identified and two representatives - C3bot from C. botulinum and C3stau2 from S. aureus - were crystallized. Here we present the 1.8A structure of C. limosum C3 transferase C3lim and compare it to the structures of other family members. In contrast to the structure of apo-C3bot, the canonical ADP-ribosylating turn turn motif is observed in a primed conformation, ready for NAD binding. This suggests an impact on the binding mode of NAD and on the transferase reaction. The crystal structure explains why auto-ADP-ribosylation of C3lim at Arg41 interferes with the ADP-ribosyltransferase activity of the toxin.
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*[[Exoenzyme 3D structures|Exoenzyme 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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3BW8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_limosum Clostridium limosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BW8 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Hathewaya limosa]]
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Crystal structure of the Clostridium limosum C3 exoenzyme., Vogelsgesang M, Stieglitz B, Herrmann C, Pautsch A, Aktories K, FEBS Lett. 2008 Apr 2;582(7):1032-6. Epub 2008 Mar 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18325337 18325337]
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[[Category: Large Structures]]
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[[Category: Clostridium limosum]]
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[[Category: Aktories K]]
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[[Category: Single protein]]
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[[Category: Herrmann C]]
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[[Category: Aktories, K.]]
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[[Category: Pautsch A]]
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[[Category: Herrmann, C.]]
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[[Category: Stieglitz B]]
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[[Category: Pautsch, A.]]
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[[Category: Vogelsgesang M]]
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[[Category: Stieglitz, B.]]
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[[Category: Vogelsgesang, M.]]
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[[Category: adp-ribosyltransferase]]
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[[Category: artt]]
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[[Category: c3bot]]
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[[Category: c3cer]]
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[[Category: c3lim]]
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[[Category: c3stau]]
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[[Category: exoenzyme c3]]
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[[Category: glycosyltransferase]]
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[[Category: nad]]
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[[Category: rho]]
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[[Category: ribosylating toxin]]
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[[Category: secreted]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 2 11:59:45 2008''
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Current revision

Crystal structure of the Clostridium limosum C3 exoenzyme

PDB ID 3bw8

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