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DAHP synthase
From Proteopedia
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== Function == | == Function == | ||
| - | '''DAHP synthase''' or '''3-deoxy-D-arabino-heptulosonate 7-phosphate synthase''' (DAHPS) catalyzes the conversion of phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate to 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) and phosphate. DAHPS is part of the shikimate pathway. DAHPS requires a bivalent metal ion cofactor for normal activity. <scene name='70/708806/Cv/6'>DAHPS is a tetramer</scene>. DAHPS exhibits feedback inhibition by aromatic amino acids like tyrosine, phenylalanine and tryptophan.<ref>PMID:1682314</ref> | + | '''DAHP synthase''' or '''3-deoxy-D-arabino-heptulosonate 7-phosphate synthase''' or '''phospho-2-dehydro-3-deoxyheptonate aldolase''' (DAHPS) catalyzes the conversion of phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate to 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) and phosphate. DAHPS is part of the shikimate pathway. DAHPS requires a bivalent metal ion cofactor for normal activity. <scene name='70/708806/Cv/6'>DAHPS is a tetramer</scene>. DAHPS exhibits feedback inhibition by aromatic amino acids like tyrosine, phenylalanine and tryptophan.<ref>PMID:1682314</ref> |
== Structural highlights == | == Structural highlights == | ||
The DAHPS active site is located in a channel at the C-terminal of the enzyme where the <scene name='70/708806/Cv/7'>substrate (PEP)</scene>, <scene name='70/708806/Cv/8'>inhibitor (phenylalanine)</scene> and <scene name='70/708806/Cv/9'>metal ion (Mn+2)</scene> are seen. The bivalent metal is bound to a Cys-X-X-His motif.<ref>PMID:12126632</ref> | The DAHPS active site is located in a channel at the C-terminal of the enzyme where the <scene name='70/708806/Cv/7'>substrate (PEP)</scene>, <scene name='70/708806/Cv/8'>inhibitor (phenylalanine)</scene> and <scene name='70/708806/Cv/9'>metal ion (Mn+2)</scene> are seen. The bivalent metal is bound to a Cys-X-X-His motif.<ref>PMID:12126632</ref> | ||
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| - | </StructureSection> | ||
== 3D Structures of DAHP synthase == | == 3D Structures of DAHP synthase == | ||
| + | [[DAHP synthase 3D structures]] | ||
| - | + | </StructureSection> | |
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| - | **[[1of8]] - yDAHPS + Co + PEP + glycerol-3-phosphate<br /> | ||
| - | **[[1rzm]] - TmDAHPS + Cd + PEP + erythrose-4-phosphate<br /> | ||
| - | **[[3pg9]] – TmDAHPS + tyrosine + inhibitor<br /> | ||
| - | **[[1kfl]] - EcDAHPS + Mn + PEP + phenylalanine <br /> | ||
| - | **[[4hso]] - MnDAHPS (mutant) + Mn + PEP + tyrosine <br /> | ||
| - | **[[5dcb]] - MnDAHPS + Mn + PEP + phenylalanine <br /> | ||
| - | **[[5czs]], [[5d05]] - MnDAHPS (mutant) + Mn + PEP + phenylalanine <br /> | ||
| - | **[[2w1a]] - MtDAHPS + Mn + TSA + chorismate mutase <br /> | ||
| - | **[[3kgf]], [[3rzi]], [[5e7z]] - MtDAHPS + Mn + phenylalanine + tryptophan<br /> | ||
| - | **[[2ypq]] - MtDAHPS + Mn + tyrosine + tryptophan<br /> | ||
| - | **[[5ckx]] - MtDAHPS + Mn + tyrosine + tryptophan<br /> | ||
| - | **[[5ckv]] - EcDAHPS + chorismate mutase + TSA + Mn + phenylalanine + tryptophan + tyrosine<br /> | ||
| - | **[[5hud]] - CgDAHPS + chorismate mutase + Mn + tryptophan<br /> | ||
| - | **[[5hue]] - CgDAHPS + Mn + PEP + tryptophan<br /> | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
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References
- ↑ Stephens CM, Bauerle R. Analysis of the metal requirement of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Escherichia coli. J Biol Chem. 1991 Nov 5;266(31):20810-7. PMID:1682314
- ↑ Shumilin IA, Zhao C, Bauerle R, Kretsinger RH. Allosteric inhibition of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase alters the coordination of both substrates. J Mol Biol. 2002 Jul 26;320(5):1147-56. PMID:12126632
