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5aff

From Proteopedia

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Symportin 1 chaperones 5S RNP assembly during ribosome biogenesis by occupying an essential rRNA binding site

Structural highlights

5aff is a 3 chain structure with sequence from Chaetomium thermophilum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.398Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

G0S5S6_CHATD

Publication Abstract from PubMed

During 60S biogenesis, mature 5S RNP consisting of 5S RNA, RpL5 and RpL11, assembles into a pre-60S particle, where docking relies on RpL11 interacting with helix 84 (H84) of the 25S RNA. How 5S RNP is assembled for recruitment into the pre-60S is not known. Here we report the crystal structure of a ternary symportin Syo1-RpL5-N-RpL11 complex and provide biochemical and structural insights into 5S RNP assembly. Syo1 guards the 25S RNA-binding surface on RpL11 and competes with H84 for binding. Pull-down experiments show that H84 releases RpL11 from the ternary complex, but not in the presence of 5S RNA. Crosslinking mass spectrometry visualizes structural rearrangements on incorporation of 5S RNA into the Syo1-RpL5-RpL11 complex supporting the formation of a pre-5S RNP. Our data underline the dual role of Syo1 in ribosomal protein transport and as an assembly platform for 5S RNP.

Symportin 1 chaperones 5S RNP assembly during ribosome biogenesis by occupying an essential rRNA-binding site.,Calvino FR, Kharde S, Ori A, Hendricks A, Wild K, Kressler D, Bange G, Hurt E, Beck M, Sinning I Nat Commun. 2015 Apr 7;6:6510. doi: 10.1038/ncomms7510. PMID:25849277^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Calvino FR, Kharde S, Ori A, Hendricks A, Wild K, Kressler D, Bange G, Hurt E, Beck M, Sinning I. Symportin 1 chaperones 5S RNP assembly during ribosome biogenesis by occupying an essential rRNA-binding site. Nat Commun. 2015 Apr 7;6:6510. doi: 10.1038/ncomms7510. PMID:25849277 doi:http://dx.doi.org/10.1038/ncomms7510

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5aff"

@@ Line 3: / Line 3: @@
 <StructureSection load='5aff' size='340' side='right'caption='[[5aff]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5aff]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_144.50 Cbs 144.50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AFF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AFF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5aff]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AFF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AFF FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aff OCA], [http://pdbe.org/5aff PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aff RCSB], [http://www.ebi.ac.uk/pdbsum/5aff PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5aff ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.398&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aff OCA], [https://pdbe.org/5aff PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aff RCSB], [https://www.ebi.ac.uk/pdbsum/5aff PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aff ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/G0S5S6_CHATD G0S5S6_CHATD]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 20: @@
 ==See Also==
-*[[Ribosomal protein L11|Ribosomal protein L11]]
+*[[Ribosomal protein L11 3D structures|Ribosomal protein L11 3D structures]]
 *[[Ribosomal protein L5|Ribosomal protein L5]]
 == References ==
@@ Line 23: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Cbs 144 50]]
+[[Category: Chaetomium thermophilum]]
 [[Category: Large Structures]]
-[[Category: Bange, G]]
+[[Category: Bange G]]
-[[Category: Calvino, F R]]
+[[Category: Calvino FR]]
-[[Category: Kharde, S]]
+[[Category: Kharde S]]
-[[Category: Sinning, I]]
+[[Category: Sinning I]]
-[[Category: Wild, K]]
+[[Category: Wild K]]
-[[Category: Alpha solenoid]]
-[[Category: Chaperone]]
-[[Category: Ribosome biogenesis]]

5aff

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Current revision

Symportin 1 chaperones 5S RNP assembly during ribosome biogenesis by occupying an essential rRNA binding site

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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