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| | <StructureSection load='4ygo' size='340' side='right'caption='[[4ygo]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='4ygo' size='340' side='right'caption='[[4ygo]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ygo]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibch Vibch]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YGO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YGO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ygo]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YGO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YGO FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MOH:METHANOL'>MOH</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3eg7|3eg7]], [[4k4l|4k4l]], [[4ncz|4ncz]], [[4mi4|4mi4]], [[4mhd|4mhd]], [[4mj8|4mj8]], [[4jly|4jly]], [[4jjx|4jjx]], [[4r57|4r57]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MOH:METHANOL'>MOH</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VC_A0947 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ygo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ygo OCA], [https://pdbe.org/4ygo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ygo RCSB], [https://www.ebi.ac.uk/pdbsum/4ygo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ygo ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ygo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ygo OCA], [http://pdbe.org/4ygo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ygo RCSB], [http://www.ebi.ac.uk/pdbsum/4ygo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ygo ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/ATDA_VIBCH ATDA_VIBCH] Involved in the protection against polyamine toxicity by regulating their concentration. Catalyzes the transfer of an acetyl group from acetyl coenzyme A (AcCoA) to the primary amino groups of spermidine to yield N(1)- and N(8)-acetylspermidine. It can use polyamines such as spermine and N(1)-acetylspermine, but not putrescine or cadaverine.<ref>PMID:23184347</ref> <ref>PMID:25623305</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4ygo" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4ygo" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Spermidine/spermine N-acetyltransferase|Spermidine/spermine N-acetyltransferase]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Vibch]] | + | [[Category: Vibrio cholerae O1 biovar El Tor str. N16961]] |
| - | [[Category: Anderson, W F]] | + | [[Category: Anderson WF]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Filippova EV]] |
| - | [[Category: Filippova, E V]] | + | [[Category: Kiryukhina O]] |
| - | [[Category: Kiryukhina, O]] | + | [[Category: Minasov G]] |
| - | [[Category: Minasov, G]] | + | |
| - | [[Category: Csgid]]
| + | |
| - | [[Category: Speg]]
| + | |
| - | [[Category: Transferase]]
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| Structural highlights
Function
ATDA_VIBCH Involved in the protection against polyamine toxicity by regulating their concentration. Catalyzes the transfer of an acetyl group from acetyl coenzyme A (AcCoA) to the primary amino groups of spermidine to yield N(1)- and N(8)-acetylspermidine. It can use polyamines such as spermine and N(1)-acetylspermine, but not putrescine or cadaverine.[1] [2]
Publication Abstract from PubMed
The spermidine N-acetyltransferase SpeG is a dodecameric enzyme that catalyzes the transfer of an acetyl group from acetyl-coenzyme A to polyamines such as spermidine and spermine. SpeG has an allosteric polyamine-binding site and acetylating polyamines regulates their intracellular concentrations. The structures of SpeG from Vibrio cholerae in complexes with polyamines and cofactor have been characterized earlier. Here, we present the dodecameric structure of SpeG from V. cholerae in a ligand-free form in three different conformational states: open, intermediate and closed. All structures were crystallized in C2 space group symmetry and contain 6 monomers in the asymmetric unit cell. Two hexamers related by crystallographic twofold symmetry form the SpeG dodecamer. The open and intermediate states have a unique open dodecameric ring. This SpeG dodecamer is asymmetric except for the one twofold axis and is unlike any known dodecameric structure. Using a fluorescence thermal shift assay, size exclusion chromatography with multi-angle light scattering, small angle X-ray scattering analysis, negative stain electron microscopy, and structural analysis we demonstrate that this unique open dodecameric state exists in solution. Our combined results indicate that polyamines trigger conformational changes and induce the symmetric closed dodecameric state of the protein when they bind to their allosteric sites.
Substrate induced allosteric change in the quaternary structure of the spermidine N-acetyltransferase SpeG.,Filippova EV, Weigand S, Osipiuk J, Kiryukhina O, Joachimiak A, Anderson WF J Mol Biol. 2015 Sep 24. pii: S0022-2836(15)00536-7. doi:, 10.1016/j.jmb.2015.09.013. PMID:26410587[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kuhn ML, Majorek KA, Minor W, Anderson WF. Broad-substrate screen as a tool to identify substrates for bacterial Gcn5-related N-acetyltransferases with unknown substrate specificity. Protein Sci. 2013 Feb;22(2):222-30. doi: 10.1002/pro.2199. Epub 2012 Dec 17. PMID:23184347 doi:http://dx.doi.org/10.1002/pro.2199
- ↑ Filippova EV, Kuhn ML, Osipiuk J, Kiryukhina O, Joachimiak A, Ballicora MA, Anderson WF. A Novel Polyamine Allosteric Site of SpeG from Vibrio cholerae Is Revealed by Its Dodecameric Structure. J Mol Biol. 2015 Mar 27;427(6 Pt B):1316-34. doi: 10.1016/j.jmb.2015.01.009. Epub, 2015 Jan 23. PMID:25623305 doi:http://dx.doi.org/10.1016/j.jmb.2015.01.009
- ↑ Filippova EV, Weigand S, Osipiuk J, Kiryukhina O, Joachimiak A, Anderson WF. Substrate induced allosteric change in the quaternary structure of the spermidine N-acetyltransferase SpeG. J Mol Biol. 2015 Sep 24. pii: S0022-2836(15)00536-7. doi:, 10.1016/j.jmb.2015.09.013. PMID:26410587 doi:http://dx.doi.org/10.1016/j.jmb.2015.09.013
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