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| | <StructureSection load='4lin' size='340' side='right'caption='[[4lin]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='4lin' size='340' side='right'caption='[[4lin]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4lin]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp22 Bpp22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LIN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4lin]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4LIN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3c9i|3c9i]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lin OCA], [http://pdbe.org/4lin PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lin RCSB], [http://www.ebi.ac.uk/pdbsum/4lin PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lin ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4lin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lin OCA], [https://pdbe.org/4lin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4lin RCSB], [https://www.ebi.ac.uk/pdbsum/4lin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4lin ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/NEEDL_BPP22 NEEDL_BPP22] Cell-perforating component and plug protein of the phage tail machine. Host cell membrane perforation allows viral DNA ejection. Together with gp4 and gp10, gp26 is required for stabilization of the condensed DNA within the capsid by plugging the hole through which the DNA enters.<ref>PMID:18059287</ref> <ref>PMID:20817910</ref> |
| - | Protein fibers are widespread in nature, but only a limited number of high-resolution structures have been determined experimentally. Unlike globular proteins, fibers are usually recalcitrant to form three-dimensional crystals, preventing single-crystal X-ray diffraction analysis. In the absence of three-dimensional crystals, X-ray fiber diffraction is a powerful tool to determine the internal symmetry of a fiber, but it rarely yields atomic resolution structural information on complex protein fibers. An 85-residue-long minimal coiled-coil repeat unit (MiCRU) was previously identified in the trimeric helical core of tail needle gp26, a fibrous protein emanating from the tail apparatus of the bacteriophage P22 virion. Here, evidence is provided that an MiCRU can be inserted in frame inside the gp26 helical core to generate a rationally extended fiber (gp26-2M) which, like gp26, retains a trimeric quaternary structure in solution. The 2.7 A resolution crystal structure of this engineered fiber, which measures approximately 320 A in length and is only 20-35 A wide, was determined. This structure, the longest for a trimeric protein fiber to be determined to such a high resolution, reveals the architecture of 22 consecutive trimerization heptads and provides a framework to decipher the structural determinants for protein fiber assembly, stability and flexibility.
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| - | | + | |
| - | Exploring the atomic structure and conformational flexibility of a 320 A long engineered viral fiber using X-ray crystallography.,Bhardwaj A, Casjens SR, Cingolani G Acta Crystallogr D Biol Crystallogr. 2014 Feb;70(Pt 2):342-53. doi:, 10.1107/S1399004713027685. Epub 2014 Jan 29. PMID:24531468<ref>PMID:24531468</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 4lin" style="background-color:#fffaf0;"></div>
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bpp22]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bhardwaj, A]] | + | [[Category: Salmonella virus P22]] |
| - | [[Category: Cingolani, G]] | + | [[Category: Bhardwaj A]] |
| - | [[Category: #945]] | + | [[Category: Cingolani G]] |
| - | [[Category: Helical coiled coil]]
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| - | [[Category: Bacteriophage p22]]
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| - | [[Category: Elongated coiled coil protein]]
| + | |
| - | [[Category: Engineered protein fiber]]
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| - | [[Category: Engineered viral protein]]
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| - | [[Category: Helical]]
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| - | [[Category: In-frame extension]]
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| - | [[Category: Protein engineering]]
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| - | [[Category: Tail needle gp26]]
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| - | [[Category: Trimeric coiled-coil]]
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| - | [[Category: Viral fiber]]
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| - | [[Category: Viral protein]]
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