5zfs

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of Arthrobacter globiformis M30 sugar epimerase which can produce D-allulose from D-fructose

Structural highlights

5zfs is a 2 chain structure with sequence from Arthrobacter globiformis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.96Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KET3E_ARTGO Catalyzes the reversible C-3 epimerization of several ketoses. Shows the highest enzymatic activity for the epimerization of L-ribulose to L-xylulose. Is also able to convert D-allulose (also known as D-psicose) to D-fructose and, to a lesser extent, L-tagatose to L-sorbose, D-ribulose to D-xylulose, L-allulose to L-fructose and D-tagatose to D-sorbose.^[1] ^[2]

Publication Abstract from PubMed

The X-ray structure of ketose 3-epimerase from Arthrobacter globiformis M30, which was previously reported to be a D-allulose 3-epimerase (AgD-AE), was determined at 1.96 A resolution. The crystal belonged to the hexagonal space group P6522, with unit-cell parameters a = b = 103.98, c = 256.53 A. The structure was solved by molecular replacement using the structure of Mesorhizobium loti L-ribulose 3-epimerase (MlL-RE), which has 41% sequence identity, as a search model. A hexagonal crystal contained two molecules in the asymmetric unit, and AgD-AE formed a homotetramer with twofold symmetry. The overall structure of AgD-AE was more similar to that of MlL-RE than to the known structures of D-psicose (alternative name D-allulose) 3-epimerases (D-PEs or D-AEs), although AgD-AE and MlL-RE have different substrate specificities. Both AgD-AE and MlL-RE have long helices in the C-terminal region that would contribute to the stability of the homotetramer. AgD-AE showed higher enzymatic activity for L-ribulose than D-allulose; however, AgD-AE is stable and is a unique useful enzyme for the production of D-allulose from D-fructose.

X-ray structure of Arthrobacter globiformis M30 ketose 3-epimerase for the production of D-allulose from D-fructose.,Yoshida H, Yoshihara A, Gullapalli PK, Ohtani K, Akimitsu K, Izumori K, Kamitori S Acta Crystallogr F Struct Biol Commun. 2018 Oct 1;74(Pt 10):669-676. doi:, 10.1107/S2053230X18011706. Epub 2018 Sep 21. PMID:30279320^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yoshihara A, Kozakai T, Shintani T, Matsutani R, Ohtani K, Iida T, Akimitsu K, Izumori K, Gullapalli PK. Purification and characterization of d-allulose 3-epimerase derived from Arthrobacter globiformis M30, a GRAS microorganism. J Biosci Bioeng. 2017 Feb;123(2):170-176. PMID:27713017 doi:10.1016/j.jbiosc.2016.09.004
↑ Yoshida H, Yoshihara A, Gullapalli PK, Ohtani K, Akimitsu K, Izumori K, Kamitori S. X-ray structure of Arthrobacter globiformis M30 ketose 3-epimerase for the production of D-allulose from D-fructose. Acta Crystallogr F Struct Biol Commun. 2018 Oct 1;74(Pt 10):669-676. doi:, 10.1107/S2053230X18011706. Epub 2018 Sep 21. PMID:30279320 doi:http://dx.doi.org/10.1107/S2053230X18011706
↑ Yoshida H, Yoshihara A, Gullapalli PK, Ohtani K, Akimitsu K, Izumori K, Kamitori S. X-ray structure of Arthrobacter globiformis M30 ketose 3-epimerase for the production of D-allulose from D-fructose. Acta Crystallogr F Struct Biol Commun. 2018 Oct 1;74(Pt 10):669-676. doi:, 10.1107/S2053230X18011706. Epub 2018 Sep 21. PMID:30279320 doi:http://dx.doi.org/10.1107/S2053230X18011706

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5zfs"

@@ Line 3: / Line 3: @@
 <StructureSection load='5zfs' size='340' side='right'caption='[[5zfs]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5zfs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZFS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ZFS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5zfs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZFS FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5zfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zfs OCA], [http://pdbe.org/5zfs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zfs RCSB], [http://www.ebi.ac.uk/pdbsum/5zfs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zfs ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zfs OCA], [https://pdbe.org/5zfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zfs RCSB], [https://www.ebi.ac.uk/pdbsum/5zfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zfs ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/KET3E_ARTGO KET3E_ARTGO] Catalyzes the reversible C-3 epimerization of several ketoses. Shows the highest enzymatic activity for the epimerization of L-ribulose to L-xylulose. Is also able to convert D-allulose (also known as D-psicose) to D-fructose and, to a lesser extent, L-tagatose to L-sorbose, D-ribulose to D-xylulose, L-allulose to L-fructose and D-tagatose to D-sorbose.<ref>PMID:27713017</ref> <ref>PMID:30279320</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Arthrobacter globiformis]]
 [[Category: Large Structures]]
-[[Category: Akimitsu, K]]
+[[Category: Akimitsu K]]
-[[Category: Gullapalli, P K]]
+[[Category: Gullapalli PK]]
-[[Category: Izumori, K]]
+[[Category: Izumori K]]
-[[Category: Kamitori, S]]
+[[Category: Kamitori S]]
-[[Category: Ohtani, K]]
+[[Category: Ohtani K]]
-[[Category: Yoshida, H]]
+[[Category: Yoshida H]]
-[[Category: Yoshihara, A]]
+[[Category: Yoshihara A]]
-[[Category: Epimerase]]
-[[Category: Isomerase]]

5zfs

From Proteopedia

Current revision

Crystal structure of Arthrobacter globiformis M30 sugar epimerase which can produce D-allulose from D-fructose

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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