8i76
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of decarboxylated osteocalcin at pH 2.0 without glycerol== | |
| + | <StructureSection load='8i76' size='340' side='right'caption='[[8i76]], [[Resolution|resolution]] 1.38Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8i76]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8I76 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8I76 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.381Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8i76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8i76 OCA], [https://pdbe.org/8i76 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8i76 RCSB], [https://www.ebi.ac.uk/pdbsum/8i76 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8i76 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/OSTCN_BOVIN OSTCN_BOVIN] Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | An acidic environment in bone is essential for bone metabolism and the production of decarboxylated osteocalcin, which functions as a regulatory hormone of glucose metabolism. Here, we describe the high-resolution X-ray crystal structure of decarboxylated osteocalcin under acidic conditions. Decarboxylated osteocalcin at pH 2.0 retains the alpha-helix structure of native osteocalcin with three gamma-carboxyglutamic acid residues at neutral pH. This implies that decarboxylated osteocalcin is stable under an acidic environment in bone. In addition, site-directed mutagenesis revealed that Glu17 and Glu21 are important for the adiponectin-inducing activity of decarboxylated osteocalcin. These findings suggest that the receptor of decarboxylated osteocalcin responds to the negative charge in helix 1 of osteocalcin. | ||
| - | + | Structural and mutational analyses of decarboxylated osteocalcin provide insight into its adiponectin-inducing activity.,Mizuguchi M, Yokoyama T, Otani T, Kuribara S, Nabeshima Y, Obita T, Hirata M, Kawano K FEBS Lett. 2023 Jun;597(11):1479-1488. doi: 10.1002/1873-3468.14618. Epub 2023 , Apr 4. PMID:36976525<ref>PMID:36976525</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8i76" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Obita | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Bos taurus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mizuguchi M]] | ||
| + | [[Category: Nabeshima Y]] | ||
| + | [[Category: Obita T]] | ||
| + | [[Category: Yokoyama T]] | ||
Current revision
Crystal structure of decarboxylated osteocalcin at pH 2.0 without glycerol
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