1rfy

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[[Image:1rfy.jpg|left|200px]]
 
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==Crystal Structure of Quorum-Sensing Antiactivator TraM==
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The line below this paragraph, containing "STRUCTURE_1rfy", creates the "Structure Box" on the page.
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<StructureSection load='1rfy' size='340' side='right'caption='[[1rfy]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1rfy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RFY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rfy OCA], [https://pdbe.org/1rfy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rfy RCSB], [https://www.ebi.ac.uk/pdbsum/1rfy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rfy ProSAT]</span></td></tr>
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{{STRUCTURE_1rfy| PDB=1rfy | SCENE= }}
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</table>
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== Function ==
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'''Crystal Structure of Quorum-Sensing Antiactivator TraM'''
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[https://www.uniprot.org/uniprot/TRAM_RHIRD TRAM_RHIRD] Negatively regulates conjugation and the expression of tra genes by antagonizing TraR/AAI-dependent activation. TraM may either bind or modify TraR or AAI making them unavailable. Alternatively, TraM may bind tra promoters preventing TraR activation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The quorum-sensing transcriptional activator TraR of Agrobacterium tumefaciens, which controls the replication and conjugal transfer of the tumour-inducing (Ti) virulence plasmid, is inhibited by the TraM antiactivator. The crystal structure of TraM reveals this protein to form a homodimer in which the monomer primarily consists of two long coiled alpha-helices, and one of the helices from each monomer also bundles to form the dimeric interface. The importance of dimerization is addressed by mutational studies in which disruption of the hydrophobic dimer interface leads to aggregation of TraM. Biochemical studies confirm that TraM exists as a homodimer in solution in equilibrium with the monomeric form, and also establish that the TraM-TraR complex is a heterodimer. Thus, the TraM homodimer undergoes dissociation in forming the antiactivation complex. Combined with the structure of TraR (Zhang et al., 2002, Nature 417: 971-974; Vannini et al., 2002, EMBO J 21: 4393-4401), our structural analysis suggests overlapping interactive surfaces in homodimeric TraM with those in the TraM-TraR complex and a mechanism for TraM inhibition on TraR.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rf/1rfy_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1RFY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RFY OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rfy ConSurf].
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Quorum-sensing antiactivator TraM forms a dimer that dissociates to inhibit TraR., Chen G, Malenkos JW, Cha MR, Fuqua C, Chen L, Mol Microbiol. 2004 Jun;52(6):1641-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15186414 15186414]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Agrobacterium tumefaciens]]
[[Category: Agrobacterium tumefaciens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cha, M R.]]
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[[Category: Cha MR]]
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[[Category: Chen, G.]]
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[[Category: Chen G]]
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[[Category: Chen, L.]]
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[[Category: Chen L]]
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[[Category: Fuqua, C.]]
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[[Category: Fuqua C]]
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[[Category: Malenkos, J W.]]
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[[Category: Malenkos JW]]
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[[Category: Homodimer]]
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[[Category: Inter- and intra-molcular two-helix coiled coil]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:27:01 2008''
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Current revision

Crystal Structure of Quorum-Sensing Antiactivator TraM

PDB ID 1rfy

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