1uoh

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(New page: 200px<br /> <applet load="1uoh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uoh, resolution 2.00&Aring;" /> '''HUMAN GANKYRIN'''<b...)
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[[Image:1uoh.gif|left|200px]]<br />
 
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<applet load="1uoh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1uoh, resolution 2.00&Aring;" />
 
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'''HUMAN GANKYRIN'''<br />
 
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==Overview==
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==HUMAN GANKYRIN==
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Gankyrin is a 25-kDa hepatocellular carcinoma-associated protein that, mediates protein-protein interactions in cell cycle control and protein, degradation. It has been reported to form complexes with cyclin-dependent, kinase 4, retinoblastoma protein, the S6b ATPase subunit of the 19 S, regulator of the 26 S proteasome, and Mdm2, an E3 ubiquitin ligase, involved in p53 degradation. It is the first protein described to bind, both to the 26 S proteasome and to proteins in other complexes containing, cyclin-dependent kinase(s) and p53 ubiquitylating activities, thus, providing a mechanism for delivering cell cycle regulating machinery and, ubiquitylated substrates to the proteasome for degradation. Gankyrin, contains a 33-residue motif known as the ankyrin repeat that occurs five, and a half to six times in the sequence. As a step toward understanding, gankyrin interactions with its protein partners we have determined its, three-dimensional crystal structure to 2.0-A resolution. It reveals that, the entire 226-residue gankyrin polypeptide folds into seven ankyrin, repeat elements. The ankyrin repeats, consisting of an antiparallel, beta-hairpin followed by a perpendicularly oriented helix-loop-helix, pack, side-by-side, creating an extended curved structure with a groove running, across the long concave surface. Comparison with the structures of other, ankyrin repeat proteins suggests that interactions with partner proteins, are mediated by residues situated on this concave surface.
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<StructureSection load='1uoh' size='340' side='right'caption='[[1uoh]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1uoh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UOH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UOH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uoh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uoh OCA], [https://pdbe.org/1uoh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uoh RCSB], [https://www.ebi.ac.uk/pdbsum/1uoh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uoh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PSD10_HUMAN PSD10_HUMAN] Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pahway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.<ref>PMID:10613832</ref> <ref>PMID:11900540</ref> <ref>PMID:11779854</ref> <ref>PMID:16023600</ref> <ref>PMID:18040287</ref> <ref>PMID:19490896</ref> <ref>PMID:19729910</ref> <ref>PMID:20628200</ref> Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export.<ref>PMID:10613832</ref> <ref>PMID:11900540</ref> <ref>PMID:11779854</ref> <ref>PMID:16023600</ref> <ref>PMID:18040287</ref> <ref>PMID:19490896</ref> <ref>PMID:19729910</ref> <ref>PMID:20628200</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uo/1uoh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uoh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Gankyrin is a 25-kDa hepatocellular carcinoma-associated protein that mediates protein-protein interactions in cell cycle control and protein degradation. It has been reported to form complexes with cyclin-dependent kinase 4, retinoblastoma protein, the S6b ATPase subunit of the 19 S regulator of the 26 S proteasome, and Mdm2, an E3 ubiquitin ligase involved in p53 degradation. It is the first protein described to bind both to the 26 S proteasome and to proteins in other complexes containing cyclin-dependent kinase(s) and p53 ubiquitylating activities, thus providing a mechanism for delivering cell cycle regulating machinery and ubiquitylated substrates to the proteasome for degradation. Gankyrin contains a 33-residue motif known as the ankyrin repeat that occurs five and a half to six times in the sequence. As a step toward understanding gankyrin interactions with its protein partners we have determined its three-dimensional crystal structure to 2.0-A resolution. It reveals that the entire 226-residue gankyrin polypeptide folds into seven ankyrin repeat elements. The ankyrin repeats, consisting of an antiparallel beta-hairpin followed by a perpendicularly oriented helix-loop-helix, pack side-by-side, creating an extended curved structure with a groove running across the long concave surface. Comparison with the structures of other ankyrin repeat proteins suggests that interactions with partner proteins are mediated by residues situated on this concave surface.
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==About this Structure==
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The crystal structure of gankyrin, an oncoprotein found in complexes with cyclin-dependent kinase 4, a 19 S proteasomal ATPase regulator, and the tumor suppressors Rb and p53.,Krzywda S, Brzozowski AM, Higashitsuji H, Fujita J, Welchman R, Dawson S, Mayer RJ, Wilkinson AJ J Biol Chem. 2004 Jan 9;279(2):1541-5. Epub 2003 Oct 22. PMID:14573599<ref>PMID:14573599</ref>
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1UOH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UOH OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The crystal structure of gankyrin, an oncoprotein found in complexes with cyclin-dependent kinase 4, a 19 S proteasomal ATPase regulator, and the tumor suppressors Rb and p53., Krzywda S, Brzozowski AM, Higashitsuji H, Fujita J, Welchman R, Dawson S, Mayer RJ, Wilkinson AJ, J Biol Chem. 2004 Jan 9;279(2):1541-5. Epub 2003 Oct 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14573599 14573599]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 1uoh" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Brzozowski, A.M.]]
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[[Category: Krzywda, S.]]
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[[Category: Wilkinson, A.J.]]
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[[Category: 26s proteasome]]
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[[Category: ankyrin repeat]]
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[[Category: oncoprotein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:37:02 2007''
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==See Also==
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*[[Ankyrin 3D structures|Ankyrin 3D structures]]
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*[[Proteasome 3D structures|Proteasome 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Brzozowski AM]]
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[[Category: Krzywda S]]
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[[Category: Wilkinson AJ]]

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HUMAN GANKYRIN

PDB ID 1uoh

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