9h4a
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Ribonuclease-three Like 4 crystallization and structure determination at room temperature in the CrystalChip== | |
| + | <StructureSection load='9h4a' size='340' side='right'caption='[[9h4a]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9h4a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9H4A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9H4A FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9h4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9h4a OCA], [https://pdbe.org/9h4a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9h4a RCSB], [https://www.ebi.ac.uk/pdbsum/9h4a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9h4a ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NFD2_ARATH NFD2_ARATH] Required for karyogamy during female gametophyte development, when the two polar nuclei fuse to form the diploid central cell nucleus.<ref>PMID:16698901</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The production of high-quality crystals is a key step in crystallography in general, but control of crystallization conditions is even more crucial in serial crystallography, which requires sets of crystals homogeneous in size and diffraction properties. This protocol describes the implementation of a simple and user-friendly microfluidic device that allows both the production of crystals by the counter-diffusion method and their in situ analysis by serial crystallography. As an illustration, the whole procedure is used to determine the crystal structure of three proteins from data collected at room temperature at a synchrotron radiation source. | ||
| - | + | Protein crystallization and structure determination at room temperature in the CrystalChip.,Pachl P, Coudray L, Vincent R, Nilles L, Scheer H, Ritzenthaler C, Fejfarova A, Rezacova P, Engilberge S, Sauter C FEBS Open Bio. 2024 Nov 21. doi: 10.1002/2211-5463.13932. PMID:39572886<ref>PMID:39572886</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 9h4a" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Ritzenthaler | + | </StructureSection> |
| - | [[Category: | + | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Scheer | + | [[Category: Large Structures]] |
| + | [[Category: Coudray L]] | ||
| + | [[Category: Engilberge S]] | ||
| + | [[Category: Nilles L]] | ||
| + | [[Category: Ritzenthaler C]] | ||
| + | [[Category: Sauter C]] | ||
| + | [[Category: Scheer H]] | ||
| + | [[Category: Vincent R]] | ||
Current revision
Ribonuclease-three Like 4 crystallization and structure determination at room temperature in the CrystalChip
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