1pma

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(New page: 200px<br /><applet load="1pma" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pma, resolution 3.4&Aring;" /> '''PROTEASOME FROM THERM...)
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[[Image:1pma.gif|left|200px]]<br /><applet load="1pma" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pma, resolution 3.4&Aring;" />
 
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'''PROTEASOME FROM THERMOPLASMA ACIDOPHILUM'''<br />
 
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==Overview==
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==PROTEASOME FROM THERMOPLASMA ACIDOPHILUM==
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The three-dimensional structure of the proteasome from the archaebacterium, Thermoplasma acidophilum has been elucidated by x-ray crystallographic, analysis by means of isomorphous replacement and cyclic averaging. The, atomic model was built and refined to a crystallographic R factor of 22.1, percent. The 673-kilodalton protease complex consists of 14 copies of two, different subunits, alpha and beta, forming a barrel-shaped structure of, four stacked rings. The two inner rings consist of seven beta subunits, each, and the two outer rings consist of seven alpha subunits each. A, narrow channel controls access to the three inner compartments. The alpha, 7 beta 7 beta 7 alpha 7 subunit assembly has 72-point group symmetry. The, structures of the alpha and beta subunits are similar, consisting of a, core of two antiparallel beta sheets that is flanked by alpha helices on, both sides. The binding of a peptide aldehyde inhibitor marks the active, site in the central cavity at the amino termini of the beta subunits and, suggests a novel proteolytic mechanism.
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<StructureSection load='1pma' size='340' side='right'caption='[[1pma]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pma]] is a 28 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. The October 2013 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Proteasome'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2013_10 10.2210/rcsb_pdb/mom_2013_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PMA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PMA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pma FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pma OCA], [https://pdbe.org/1pma PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pma RCSB], [https://www.ebi.ac.uk/pdbsum/1pma PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pma ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PSA_THEAC PSA_THEAC] Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The T.acidophilum proteasome is able to cleave oligopeptides after Tyr, Leu, Phe, and to a lesser extent after Glu and Arg. Thus, displays chymotrypsin-like activity and low level of caspase-like and trypsin-like activities.<ref>PMID:8999862</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pm/1pma_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pma ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1PMA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Active as [http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PMA OCA].
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*[[Proteasome 3D structures|Proteasome 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of the 20S proteasome from the archaeon T. acidophilum at 3.4 A resolution., Lowe J, Stock D, Jap B, Zwickl P, Baumeister W, Huber R, Science. 1995 Apr 28;268(5210):533-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7725097 7725097]
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__TOC__
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[[Category: Proteasome endopeptidase complex]]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Proteasome]]
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[[Category: RCSB PDB Molecule of the Month]]
[[Category: Thermoplasma acidophilum]]
[[Category: Thermoplasma acidophilum]]
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[[Category: Baumeister, W.]]
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[[Category: Baumeister W]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: Jap, B.]]
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[[Category: Jap B]]
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[[Category: Loewe, J.]]
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[[Category: Loewe J]]
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[[Category: Stock, D.]]
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[[Category: Stock D]]
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[[Category: Zwickl, P.]]
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[[Category: Zwickl P]]
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[[Category: hydrolase]]
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[[Category: protease]]
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[[Category: proteasome]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:55:12 2007''
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Current revision

PROTEASOME FROM THERMOPLASMA ACIDOPHILUM

PDB ID 1pma

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