1rfy

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(New page: 200px<br /><applet load="1rfy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rfy, resolution 1.60&Aring;" /> '''Crystal Structure of...)
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[[Image:1rfy.jpg|left|200px]]<br /><applet load="1rfy" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rfy, resolution 1.60&Aring;" />
 
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'''Crystal Structure of Quorum-Sensing Antiactivator TraM'''<br />
 
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==Overview==
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==Crystal Structure of Quorum-Sensing Antiactivator TraM==
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The quorum-sensing transcriptional activator TraR of Agrobacterium, tumefaciens, which controls the replication and conjugal transfer of the, tumour-inducing (Ti) virulence plasmid, is inhibited by the TraM, antiactivator. The crystal structure of TraM reveals this protein to form, a homodimer in which the monomer primarily consists of two long coiled, alpha-helices, and one of the helices from each monomer also bundles to, form the dimeric interface. The importance of dimerization is addressed by, mutational studies in which disruption of the hydrophobic dimer interface, leads to aggregation of TraM. Biochemical studies confirm that TraM exists, as a homodimer in solution in equilibrium with the monomeric form, and, also establish that the TraM-TraR complex is a heterodimer. Thus, the TraM, homodimer undergoes dissociation in forming the antiactivation complex., Combined with the structure of TraR (Zhang et al., 2002, Nature 417:, 971-974; Vannini et al., 2002, EMBO J 21: 4393-4401), our structural, analysis suggests overlapping interactive surfaces in homodimeric TraM, with those in the TraM-TraR complex and a mechanism for TraM inhibition on, TraR.
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<StructureSection load='1rfy' size='340' side='right'caption='[[1rfy]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1rfy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RFY FirstGlance]. <br>
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1RFY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RFY OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rfy OCA], [https://pdbe.org/1rfy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rfy RCSB], [https://www.ebi.ac.uk/pdbsum/1rfy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rfy ProSAT]</span></td></tr>
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==Reference==
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</table>
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Quorum-sensing antiactivator TraM forms a dimer that dissociates to inhibit TraR., Chen G, Malenkos JW, Cha MR, Fuqua C, Chen L, Mol Microbiol. 2004 Jun;52(6):1641-51. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15186414 15186414]
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== Function ==
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[https://www.uniprot.org/uniprot/TRAM_RHIRD TRAM_RHIRD] Negatively regulates conjugation and the expression of tra genes by antagonizing TraR/AAI-dependent activation. TraM may either bind or modify TraR or AAI making them unavailable. Alternatively, TraM may bind tra promoters preventing TraR activation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rf/1rfy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rfy ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Agrobacterium tumefaciens]]
[[Category: Agrobacterium tumefaciens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cha, M.R.]]
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[[Category: Cha MR]]
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[[Category: Chen, G.]]
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[[Category: Chen G]]
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[[Category: Chen, L.]]
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[[Category: Chen L]]
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[[Category: Fuqua, C.]]
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[[Category: Fuqua C]]
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[[Category: Malenkos, J.W.]]
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[[Category: Malenkos JW]]
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[[Category: inter- and intra-molcular two-helix coiled coil; homodimer]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:33:55 2007''
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Current revision

Crystal Structure of Quorum-Sensing Antiactivator TraM

PDB ID 1rfy

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