1crx
From Proteopedia
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[[Image:1crx.png|left|200px]] | [[Image:1crx.png|left|200px]] | ||
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{{STRUCTURE_1crx| PDB=1crx | SCENE= }} | {{STRUCTURE_1crx| PDB=1crx | SCENE= }} | ||
===CRE RECOMBINASE/DNA COMPLEX REACTION INTERMEDIATE I=== | ===CRE RECOMBINASE/DNA COMPLEX REACTION INTERMEDIATE I=== | ||
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==See Also== | ==See Also== | ||
| - | *[[Resolvase]] | + | *[[Resolvase|Resolvase]] |
==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID: | + | <ref group="xtra">PMID:009288963</ref><references group="xtra"/> |
[[Category: Enterobacteria phage p1]] | [[Category: Enterobacteria phage p1]] | ||
[[Category: Duyne, G D.Van.]] | [[Category: Duyne, G D.Van.]] | ||
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[[Category: Protein:dna complex]] | [[Category: Protein:dna complex]] | ||
[[Category: Reaction intermediate]] | [[Category: Reaction intermediate]] | ||
| - | [[Category: Replication | + | [[Category: Replication-dna complex]] |
[[Category: Site-specific recombinase]] | [[Category: Site-specific recombinase]] | ||
Revision as of 13:25, 25 July 2012
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| 1crx, resolution 2.40Å () | |||||||||
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| Ligands: | |||||||||
| Gene: | CRE (Enterobacteria phage P1) | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
CRE RECOMBINASE/DNA COMPLEX REACTION INTERMEDIATE I
During site-specific DNA recombination, which brings about genetic rearrangement in processes such as viral integration and excision and chromosomal segregation, recombinase enzymes recognize specific DNA sequences and catalyse the reciprocal exchange of DNA strands between these sites. The bacteriophage recombinase Cre catalyses site-specific recombination between two 34-base-pair loxP sites. The crystal structure at 2.4 A resolution of Cre bound to a loxP substrate reveals an intermediate in the recombination reaction, in which a Cre molecule has cleaved the substrate to form a covalent 3'-phosphotyrosine linkage with the DNA. Four recombinases and two loxP sites form a synapsed structure in which the DNA resembles models of four-way Holliday-Junction intermediates. The Cre-loxP complex challenges models of site-specific recombination that require large changes in quaternary structure. Subtle allosteric changes at the carboxy termini of the Cre subunits may instead coordinate the cleavage and strand-exchange reactions.
Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse., Guo F, Gopaul DN, van Duyne GD, Nature. 1997 Sep 4;389(6646):40-6. PMID:9288963
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1crx is a 6 chain structure of Resolvase with sequence from Enterobacteria phage p1. Full crystallographic information is available from OCA.
See Also
Reference
- Guo F, Gopaul DN, van Duyne GD. Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse. Nature. 1997 Sep 4;389(6646):40-6. PMID:9288963 doi:10.1038/37925
