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Chaperonin
From Proteopedia
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== 3D Structures of Chaperonin == | == 3D Structures of Chaperonin == | ||
| - | ''Updated | + | ''Updated June 2012'' |
===Group I=== | ===Group I=== | ||
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[[3c9v]], [[3cau]] – EcGroEL – Cryo EM<BR /> | [[3c9v]], [[3cau]] – EcGroEL – Cryo EM<BR /> | ||
[[2c7e]], [[1gr5]] - EcGroEL (mutant) – Cryo EM<BR /> | [[2c7e]], [[1gr5]] - EcGroEL (mutant) – Cryo EM<BR /> | ||
| + | [[1dk7]] - EcGroEL apical domain<br /> | ||
| + | [[1fy9]], [[1fya]], [[1kid]], [[1jon]] – EcGroEL apical domain (mutant) <BR /> | ||
| + | [[1srv]] – TtCPN60 apical domain - ''Thermus thermophilus''<BR /> | ||
| + | [[3osx]] - CPN60 apical domain – ''Xenorhabdus nematophila''<br /> | ||
| + | [[1iok]] – CPN60 – ''Paracoccus denitrificans'' | ||
| + | |||
| + | ''Large subunit binary complex'' | ||
| + | |||
[[1kp8]] - EcGroEL (mutant) + ATP<BR /> | [[1kp8]] - EcGroEL (mutant) + ATP<BR /> | ||
[[1sx3]] - EcGroEL + ATP<BR /> | [[1sx3]] - EcGroEL + ATP<BR /> | ||
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[[1mnf]] – EcGroEL + polypeptide<BR /> | [[1mnf]] – EcGroEL + polypeptide<BR /> | ||
[[2cgt]] – EcGroEL + capsid assbly protein GP31 – EM<BR /> | [[2cgt]] – EcGroEL + capsid assbly protein GP31 – EM<BR /> | ||
| - | [[ | + | [[1dkd]] - EcGroEL apical domain + polypeptide |
| - | + | ||
| - | + | ||
| - | + | ||
''Small subunit'' | ''Small subunit'' | ||
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[[3izh]], [[3izi]], [[3izj]], [[3izk]], [[3izl]], [[3izm]], [[3izn]], [[3los]], [[3iyf]] – MmCPN – ''Methanococcus maripaludis'' – EM<BR /> | [[3izh]], [[3izi]], [[3izj]], [[3izk]], [[3izl]], [[3izm]], [[3izn]], [[3los]], [[3iyf]] – MmCPN – ''Methanococcus maripaludis'' – EM<BR /> | ||
[[3kfb]], [[3kfe]], [[3kfk]] – MmCPN<BR /> | [[3kfb]], [[3kfe]], [[3kfk]] – MmCPN<BR /> | ||
| + | [[3j02]], [[3j03]] – MmCPN (mutant) – Cryo EM<br /> | ||
| + | [[3ruq]] - MmCPN + ADP<br /> | ||
| + | [[3rus]] - MmCPN (mutant) + ADP<br /> | ||
| + | [[3ruw]] - MmCPN (mutant) + ADP-AlF3<br /> | ||
| + | [[3ruv]] - MmCPN (mutant) + ATP analog<br /> | ||
[[1q2v]], [[1q3r]] – TkCPN α subunit (mutant) – ''Thermococcus'' KS-1<BR /> | [[1q2v]], [[1q3r]] – TkCPN α subunit (mutant) – ''Thermococcus'' KS-1<BR /> | ||
[[1q3q]] - TkCPN α subunit (mutant) + AMP-PNP<BR /> | [[1q3q]] - TkCPN α subunit (mutant) + AMP-PNP<BR /> | ||
Revision as of 08:15, 5 June 2012
Chaperonin (CPN) are oligomeric proteins which mediate the folding of polypeptide chains. Group I CPNs are found in bacteria, chloroplasts and mitochondria. They include the most characterized GroEL/GroES complex from Escherichia coli and CPN60/CPN10 from Thermus thermophilus. The larger subunit (GroEL, CPN60) contains 3 domains. The apical domain is the one which binds the substrate. Group II CPNs are found in eukaryotic cytosol and archaea. Thermosome is a CPN complex found in archaea. CCT is a CPN complex found in eukarya.
3D Structures of Chaperonin
Updated June 2012
Group I
Large subunit
3m6c – MtGroEL1 apical domain – Mycobacterium tuberculosis
1sjp, 3rtk – MtGroEL2 residues 42-539
3fbh, 2eu1, 1j4z, 1kpo, 1oel, 1grl, 2yey – EcGroEL (mutant) - Escherichia coli
3e76, 2nwc, 1xck, 1ss8 – EcGroEL
3c9v, 3cau – EcGroEL – Cryo EM
2c7e, 1gr5 - EcGroEL (mutant) – Cryo EM
1dk7 - EcGroEL apical domain
1fy9, 1fya, 1kid, 1jon – EcGroEL apical domain (mutant)
1srv – TtCPN60 apical domain - Thermus thermophilus
3osx - CPN60 apical domain – Xenorhabdus nematophila
1iok – CPN60 – Paracoccus denitrificans
Large subunit binary complex
1kp8 - EcGroEL (mutant) + ATP
1sx3 - EcGroEL + ATP
1sx4 - EcGroEL + ADP
1mnf – EcGroEL + polypeptide
2cgt – EcGroEL + capsid assbly protein GP31 – EM
1dkd - EcGroEL apical domain + polypeptide
Small subunit
3nx6 – GroES residues 40-134 (mutant) – Xanthomonas oryzae
1wnr – TtCPN10 residues 1-94
1p3h, 1hx5 – MtCPN10
1p82, 1p83 - MtCPN10 residues 1-25 - NMR
1egs - GroES residues 19-27 - NMR
Large + small subunit
1svt, 1pcq - EcGroEL + GroES
1pf9, 1aon - EcGroEL + GroES + ADP
2c7c, 2c7d - EcGroEL + GroES – Cryo EM
1gru - EcGroEL + GroES + ATP + ADP – Cryo EM
1we3 – TtCPN60 + CPN10
1wf4 - TtCPN60 + CPN10 + ADP
Group II
3izh, 3izi, 3izj, 3izk, 3izl, 3izm, 3izn, 3los, 3iyf – MmCPN – Methanococcus maripaludis – EM
3kfb, 3kfe, 3kfk – MmCPN
3j02, 3j03 – MmCPN (mutant) – Cryo EM
3ruq - MmCPN + ADP
3rus - MmCPN (mutant) + ADP
3ruw - MmCPN (mutant) + ADP-AlF3
3ruv - MmCPN (mutant) + ATP analog
1q2v, 1q3r – TkCPN α subunit (mutant) – Thermococcus KS-1
1q3q - TkCPN α subunit (mutant) + AMP-PNP
1q3s - TkCPN α subunit (mutant) + ADP
CCT
2xsm – bCPN CCT – bovine
3p9d, 3p9e – CPN CCT - yeast
3iyg - bCPN CCT – Cryo EM
3ktt - bCPN CCT β subunit – Cryo EM
1gml, 1gn1 - CPN CCT γ subunit apical domain – mouse
Hsp33
3m7m, 1hw7 – EcCPN Hsp33 N-terminal
1xjh - EcCPN Hsp33 C terminal - NMR
1vq0 - CPN Hsp33 – Thermotoga maritima
1vzy - CPN Hsp33 (mutant) – Bacillus subtilis
Thermosome
1a6d - TaTherm α+β subunits – Thermoplasma acidophilum
1a6e - TaTherm α+β subunits + ADP
1ass, 1asx - TaTherm α apical domain
1e0r – TaTherm β apical domain
3ko1 – Therm – Acidianus tengchongensis
3aq1 – Therm – Methanococcoides burtonii
1lep – CPN-10 – Mycobacterium leprae
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