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1umg

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[[Image:1umg.gif|left|200px]]<br /><applet load="1umg" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1umg.gif|left|200px]]
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caption="1umg, resolution 1.80&Aring;" />
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'''Crystal strucure of fructose-1,6-bisphosphatase'''<br />
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{{Structure
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|PDB= 1umg |SIZE=350|CAPTION= <scene name='initialview01'>1umg</scene>, resolution 1.80&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=2FP:1,6-FRUCTOSE+DIPHOSPHATE+(LINEAR+FORM)'>2FP</scene> and <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Fructose-bisphosphatase Fructose-bisphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.11 3.1.3.11]
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|GENE= ST0318 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 Sulfolobus tokodaii])
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}}
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'''Crystal strucure of fructose-1,6-bisphosphatase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1UMG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=2FP:'>2FP</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Fructose-bisphosphatase Fructose-bisphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.11 3.1.3.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UMG OCA].
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1UMG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UMG OCA].
==Reference==
==Reference==
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The first crystal structure of the novel class of fructose-1,6-bisphosphatase present in thermophilic archaea., Nishimasu H, Fushinobu S, Shoun H, Wakagi T, Structure. 2004 Jun;12(6):949-59. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15274916 15274916]
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The first crystal structure of the novel class of fructose-1,6-bisphosphatase present in thermophilic archaea., Nishimasu H, Fushinobu S, Shoun H, Wakagi T, Structure. 2004 Jun;12(6):949-59. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15274916 15274916]
[[Category: Fructose-bisphosphatase]]
[[Category: Fructose-bisphosphatase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: three metal-assisted mechanism]]
[[Category: three metal-assisted mechanism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:26:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:33:54 2008''

Revision as of 12:33, 20 March 2008

Image:1umg.gif


PDB ID 1umg

Drag the structure with the mouse to rotate
, resolution 1.80Å
Ligands: , and
Gene: ST0318 (Sulfolobus tokodaii)
Activity: Fructose-bisphosphatase, with EC number 3.1.3.11
Coordinates: save as pdb, mmCIF, xml



Crystal strucure of fructose-1,6-bisphosphatase


Overview

As the first structure of the novel class of fructose-1,6-bisphosphatase (FBPase) present in thermophilic archaea, we solved the crystal structure of the ST0318 gene product (St-Fbp) of Sulfolobus tokodaii strain 7. The St-Fbp structure comprises a homooctamer of the 422 point-group. The protein folds as a four-layer alpha-beta-beta-alpha sandwich with a novel topology, which is completely different from the sugar phosphatase fold. The structure contains an unhydrolyzed FBP molecule in the open-keto form, as well as four hexacoordinated magnesium ions around the 1-phosphoryl group of FBP. The arrangement of the catalytic side chains and metal ligands is consistent with the three-metal ion assisted catalysis proposed for conventional FBPases. The structure provides an insight into the structural basis of the strict substrate specificity of St-Fbp.

About this Structure

1UMG is a Single protein structure of sequence from Sulfolobus tokodaii. Full crystallographic information is available from OCA.

Reference

The first crystal structure of the novel class of fructose-1,6-bisphosphatase present in thermophilic archaea., Nishimasu H, Fushinobu S, Shoun H, Wakagi T, Structure. 2004 Jun;12(6):949-59. PMID:15274916

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