1q31
From Proteopedia
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| - | + | ==Crystal Structure of the Tobacco Etch Virus Protease C151A mutant== | |
| - | + | <StructureSection load='1q31' size='340' side='right' caption='[[1q31]], [[Resolution|resolution]] 2.70Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[1q31]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Tobacco_etch_virus Tobacco etch virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Q31 FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lvb|1lvb]], [[1lvm|1lvm]]</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nuclear-inclusion-a_endopeptidase Nuclear-inclusion-a endopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.44 3.4.22.44] </span></td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1q31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q31 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1q31 RCSB], [http://www.ebi.ac.uk/pdbsum/1q31 PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q3/1q31_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Tobacco etch virus (TEV) protease is a cysteine protease exhibiting stringent sequence specificity. The enzyme is widely used in biotechnology for the removal of the affinity tags from recombinant fusion proteins. Crystal structures of two TEV protease mutants as complexes with a substrate and a product peptide provided the first insight into the mechanism of substrate specificity of this enzyme. We now report a 2.7A crystal structure of a full-length inactive C151A mutant protein crystallised in the absence of peptide. The structure reveals the C terminus of the protease bound to the active site. In addition, we determined dissociation constants of TEV protease substrate and product peptides using isothermal titration calorimetry for various forms of this enzyme. Data suggest that TEV protease could be inhibited by the peptide product of autolysis. Separate modes of recognition for native substrates and the site of TEV protease self-cleavage are proposed. | ||
| - | + | Crystal structure of tobacco etch virus protease shows the protein C terminus bound within the active site.,Nunn CM, Jeeves M, Cliff MJ, Urquhart GT, George RR, Chao LH, Tscuchia Y, Djordjevic S J Mol Biol. 2005 Jul 1;350(1):145-55. PMID:15919091<ref>PMID:15919091</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Ashley Steere/Tobacco Etch Virus (TEV) Protease|Ashley Steere/Tobacco Etch Virus (TEV) Protease]] | *[[Ashley Steere/Tobacco Etch Virus (TEV) Protease|Ashley Steere/Tobacco Etch Virus (TEV) Protease]] | ||
*[[Tobacco Etch Virus (TEV) Protease|Tobacco Etch Virus (TEV) Protease]] | *[[Tobacco Etch Virus (TEV) Protease|Tobacco Etch Virus (TEV) Protease]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Nuclear-inclusion-a endopeptidase]] | [[Category: Nuclear-inclusion-a endopeptidase]] | ||
[[Category: Tobacco etch virus]] | [[Category: Tobacco etch virus]] | ||
Revision as of 15:10, 29 September 2014
Crystal Structure of the Tobacco Etch Virus Protease C151A mutant
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