4g7e
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal structure of pigeon pea urease== | |
| - | === | + | <StructureSection load='4g7e' size='340' side='right' caption='[[4g7e]], [[Resolution|resolution]] 2.20Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4g7e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cajanus_cajan Cajanus cajan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G7E FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g7e RCSB], [http://www.ebi.ac.uk/pdbsum/4g7e PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Urease is an enzyme that catalyzes the hydrolysis of urea, forming ammonia and carbon dioxide, and is found in plants, microorganisms and invertebrates. Although plant and bacterial ureases are closely related at amino acid and at the structural level, the insecticidal activity is seen only in the plant ureases. In contrast, both plant and bacterial ureases exhibit antifungal activity. These two biological properties are independent of its ureolytic activity. However, till date the mechanism(s) behind the insecticidal and fungicidal activity of ureases are not clearly understood. Here we report the crystal structure of pigeon pea urease (PPU, Cajanus cajan) which is the second structure from the plant source. We have deduced the amino acid sequence of PPU and also report here studies on its stability, insecticidal and antifungal activity. PPU exhibits cellulase activity. Based on the structural analysis of PPU and docking studies with cellopentoase we propose a possible mechanism of antifungal activity of urease. | ||
| - | + | Structural and functional studies on urease from pigeon pea (Cajanus cajan).,Balasubramanian A, Durairajpandian V, Elumalai S, Mathivanan N, Munirajan AK, Ponnuraj K Int J Biol Macromol. 2013 Jul;58:301-9. doi: 10.1016/j.ijbiomac.2013.04.055. Epub, 2013 Apr 24. PMID:23624166<ref>PMID:23624166</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | |||
| + | ==See Also== | ||
| + | *[[Urease|Urease]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Cajanus cajan]] | [[Category: Cajanus cajan]] | ||
[[Category: Urease]] | [[Category: Urease]] | ||
| - | [[Category: Balasubramanian, A | + | [[Category: Balasubramanian, A]] |
| - | [[Category: Ponnuraj, K | + | [[Category: Ponnuraj, K]] |
[[Category: B domain]] | [[Category: B domain]] | ||
[[Category: Carbon di-oxide]] | [[Category: Carbon di-oxide]] | ||
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[[Category: Pigeon pea]] | [[Category: Pigeon pea]] | ||
[[Category: Tim barrel domain]] | [[Category: Tim barrel domain]] | ||
| - | [[Category: Urease]] | ||
Revision as of 11:11, 21 December 2014
Crystal structure of pigeon pea urease
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