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NF-Y Transcription Factor Sandbox
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| - | NF-Y transcription factor | + | NF-Y transcription factor consists of NF-YA, NF-YB, and NFY-C subunits composed of two α helices each. The N terminal A1 helix of the NF-YA subunit interacts with NF-YB and NF-YC subunits resulting in a heterotrimer. The NF-Y heterotrimer is stabilized by ionic interactions, interactions between backbones the backbone atoms of residues, and hydrophobic residues. Stabilizing ionic interactions occur between Asn239(NF-YA) with Asp109(NF-YC) and Asp112(NF-YC). Residue backbone interactions occur between Leu123(NF-YB) with Phe113(NF-YC), Arg245(NF-YA) with Glu98(NF-YB) and Glu101(NF-YB), Arg249(NF-YA) with Glu90(NF-YB), and Arg250(NF-YA) with Asp116(NF-YC). Hydrophobic residues that contributed to the stabilization of the NF-Y heterotrimer are only located at NF-YA and NF-YB subunits at residues Ile246(NF-YA), Phe94(NF-YB), and Ile115(NF-YB). |
== Interaction With DNA == | == Interaction With DNA == | ||
Revision as of 03:42, 5 November 2013
Protein Function
Protein Structure
NF-Y transcription factor consists of NF-YA, NF-YB, and NFY-C subunits composed of two α helices each. The N terminal A1 helix of the NF-YA subunit interacts with NF-YB and NF-YC subunits resulting in a heterotrimer. The NF-Y heterotrimer is stabilized by ionic interactions, interactions between backbones the backbone atoms of residues, and hydrophobic residues. Stabilizing ionic interactions occur between Asn239(NF-YA) with Asp109(NF-YC) and Asp112(NF-YC). Residue backbone interactions occur between Leu123(NF-YB) with Phe113(NF-YC), Arg245(NF-YA) with Glu98(NF-YB) and Glu101(NF-YB), Arg249(NF-YA) with Glu90(NF-YB), and Arg250(NF-YA) with Asp116(NF-YC). Hydrophobic residues that contributed to the stabilization of the NF-Y heterotrimer are only located at NF-YA and NF-YB subunits at residues Ile246(NF-YA), Phe94(NF-YB), and Ile115(NF-YB).
Interaction With DNA
The C terminal of the NF-YA subunit inserts deep into the minor groove of DNA. NF-YA A2 helix binds to the box and causes the minor groove to widen at the CCAAT box. An important residue in the catalytic site is . . . . . . .
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