1umg
From Proteopedia
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|PDB= 1umg |SIZE=350|CAPTION= <scene name='initialview01'>1umg</scene>, resolution 1.80Å | |PDB= 1umg |SIZE=350|CAPTION= <scene name='initialview01'>1umg</scene>, resolution 1.80Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=2FP:1,6-FRUCTOSE+DIPHOSPHATE+(LINEAR+FORM)'>2FP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Fructose-bisphosphatase Fructose-bisphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.11 3.1.3.11] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphatase Fructose-bisphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.11 3.1.3.11] </span> |
|GENE= ST0318 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 Sulfolobus tokodaii]) | |GENE= ST0318 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 Sulfolobus tokodaii]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1umg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1umg OCA], [http://www.ebi.ac.uk/pdbsum/1umg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1umg RCSB]</span> | ||
}} | }} | ||
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[[Category: Shoun, H.]] | [[Category: Shoun, H.]] | ||
[[Category: Wakagi, T.]] | [[Category: Wakagi, T.]] | ||
| - | [[Category: 2FP]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: MPD]] | ||
| - | [[Category: 6-bisphosphatase]] | ||
[[Category: alpha-beta-beta-alpha four layer sandwich]] | [[Category: alpha-beta-beta-alpha four layer sandwich]] | ||
| - | [[Category: fructose-1]] | + | [[Category: fructose-1,6-bisphosphatase]] |
[[Category: hyperthermophilic archaea]] | [[Category: hyperthermophilic archaea]] | ||
[[Category: magnesium ion]] | [[Category: magnesium ion]] | ||
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[[Category: three metal-assisted mechanism]] | [[Category: three metal-assisted mechanism]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:12:16 2008'' |
Revision as of 21:12, 30 March 2008
| |||||||
| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | ST0318 (Sulfolobus tokodaii) | ||||||
| Activity: | Fructose-bisphosphatase, with EC number 3.1.3.11 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal strucure of fructose-1,6-bisphosphatase
Overview
As the first structure of the novel class of fructose-1,6-bisphosphatase (FBPase) present in thermophilic archaea, we solved the crystal structure of the ST0318 gene product (St-Fbp) of Sulfolobus tokodaii strain 7. The St-Fbp structure comprises a homooctamer of the 422 point-group. The protein folds as a four-layer alpha-beta-beta-alpha sandwich with a novel topology, which is completely different from the sugar phosphatase fold. The structure contains an unhydrolyzed FBP molecule in the open-keto form, as well as four hexacoordinated magnesium ions around the 1-phosphoryl group of FBP. The arrangement of the catalytic side chains and metal ligands is consistent with the three-metal ion assisted catalysis proposed for conventional FBPases. The structure provides an insight into the structural basis of the strict substrate specificity of St-Fbp.
About this Structure
1UMG is a Single protein structure of sequence from Sulfolobus tokodaii. Full crystallographic information is available from OCA.
Reference
The first crystal structure of the novel class of fructose-1,6-bisphosphatase present in thermophilic archaea., Nishimasu H, Fushinobu S, Shoun H, Wakagi T, Structure. 2004 Jun;12(6):949-59. PMID:15274916
Page seeded by OCA on Mon Mar 31 00:12:16 2008
Categories: Fructose-bisphosphatase | Single protein | Sulfolobus tokodaii | Fushinobu, S. | Nishimasu, H. | Shoun, H. | Wakagi, T. | Alpha-beta-beta-alpha four layer sandwich | Fructose-1,6-bisphosphatase | Hyperthermophilic archaea | Magnesium ion | Octamer | Phosphatase | Three metal-assisted mechanism
