4m24

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==Crystal structure of the endo-1,4-glucanase, RBcel1, in complex with cellobiose==
==Crystal structure of the endo-1,4-glucanase, RBcel1, in complex with cellobiose==
<StructureSection load='4m24' size='340' side='right' caption='[[4m24]], [[Resolution|resolution]] 1.39&Aring;' scene=''>
<StructureSection load='4m24' size='340' side='right' caption='[[4m24]], [[Resolution|resolution]] 1.39&Aring;' scene=''>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ee9|4ee9]]</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ee9|4ee9]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m24 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4m24 RCSB], [http://www.ebi.ac.uk/pdbsum/4m24 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4m24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m24 OCA], [http://pdbe.org/4m24 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4m24 RCSB], [http://www.ebi.ac.uk/pdbsum/4m24 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4m24 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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In this study, the mining of an Antarctic soil sample by functional metagenomics allowed the isolation of a cold-adapted protein (RBcel1) that hydrolyzes only carboxymethyl cellulose. The new enzyme is related to family 5 of the glycosyl hydrolase (GH5) protein from Pseudomonas stutzeri (Pst_2494) and does not possess a carbohydrate-binding domain. The protein was produced and purified to homogeneity. RBcel1 displayed an endoglucanase activity, producing cellobiose and cellotriose, using carboxymethyl cellulose as a substrate. Moreover, the study of pH and the thermal dependence of the hydrolytic activity shows that RBcel1 was active from pH 6 to pH 9 and remained significantly active when temperature decreased (18% of activity at 10 degrees C). It is interesting that RBcel1 was able to synthetize non-reticulated cellulose using cellobiose as a substrate. Moreover, by a combination of bioinformatics and enzyme analysis, the physiological relevance of the RBcel1 protein and its mesophilic homologous Pst_2494 protein from P. stutzeri, A1501, was established as the key enzymes involved in the production of cellulose by bacteria. In addition, RBcel1 and Pst_2494 are the two primary enzymes belonging to the GH5 family involved in this process.
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RBcel1 is an endoglucanase belonging to glycoside hydrolase family 5 subfamily 5 (GH5_5) that was recently identified from a soil metagenome library from the Antarctic. Unlike its closest structural homologue (Cel5A from Thermoascus aurantiacus), this enzyme was reported to be able to catalyze transglycosylation reactions and has putatively been implicated in the bacterial cellulose-synthesis process. Here, the structure of RBcel1 at 1.4 A resolution, solved by molecular replacement, is reported. The structure and putative substrate-binding site are described and compared with those of other GH5_5 subfamily members.
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Insights into bacterial cellulose biosynthesis by functional metagenomics on Antarctic soil samples.,Berlemont R, Delsaute M, Pipers D, D'Amico S, Feller G, Galleni M, Power P ISME J. 2009 Sep;3(9):1070-81. doi: 10.1038/ismej.2009.48. Epub 2009 May 21. PMID:19458657<ref>PMID:19458657</ref>
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Three-dimensional structure of RBcel1, a metagenome-derived psychrotolerant family GH5 endoglucanase.,Delsaute M, Berlemont R, Dehareng D, Van Elder D, Galleni M, Bauvois C Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Aug;69(Pt 8):828-33. doi: , 10.1107/S1744309113014565. Epub 2013 Jul 26. PMID:23908022<ref>PMID:23908022</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4m24" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Glucanase|Glucanase]]
== References ==
== References ==
<references/>
<references/>

Revision as of 16:25, 4 August 2016

Crystal structure of the endo-1,4-glucanase, RBcel1, in complex with cellobiose

4m24, resolution 1.39Å

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