Glucanase

From Proteopedia

spinqualitylabelsall models Endo-1,3-β-glucanase complex with glycerol, phosphate and Ca+2 ion, 3atg Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Glucanases (Gase) are enzymes which break down glucans which are part of plant cell wall. Gase hydrolyzes the 1,4-β-glycosidic link in cellulose, lichenin and β-D-glucan. Endo-Gase breaks internal cellulose bonds[1]. Exo-Gase cleaves 2 to 4 units from the ends of exposed cellulose polysaccharide chains[2]. *Xyloglucanase (XGase) breaks down the hemicellulose xyloglucan[3]. alpha-1,3-glucanase hydrolyzes alpha-1,3-glucan [4]. beta-1,3-glucanase enhances fungal resistance in plants[5]. Gase are classified according to polysaccharide bond which they break. For details see *Molecular Playground/1,3-1,4-beta-D-glucanase *Crystal Structure Determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A *(1→3)-α-Glucanases: An Alternative to Control Dental Plaque and Improve Oral Health. 3D structures of glucanase Glucanase 3D structures

References

1. ↑ Barras DR, Stone BA. Beta-1,3-glucan hydrolases from Euglena gracilis. I. The nature of the hydrolases. Biochim Biophys Acta. 1969 Nov 4;191(2):329-41. PMID:5354264
2. ↑ Tsai LC, Amiraslanov I, Chen HR, Chen YW, Lee HL, Liang PH, Liaw YC. Structures of exoglucanase from Clostridium cellulovorans: cellotetraose binding and cleavage. Acta Crystallogr F Struct Biol Commun. 2015 Oct 1;71(Pt 10):1264-72. doi:, 10.1107/S2053230X15015915. Epub 2015 Sep 23. PMID:26457517 doi:http://dx.doi.org/10.1107/S2053230X15015915
3. ↑ Vincken JP, Beldman G, Voragen AG. Substrate specificity of endoglucanases: what determines xyloglucanase activity? Carbohydr Res. 1997 Mar 13;298(4):299-310. PMID:9098958
4. ↑ Suyotha W, Yano S, Wakayama M. α-1,3-Glucanase: present situation and prospect of research. World J Microbiol Biotechnol. 2016 Feb;32(2):30. PMID:26748807 doi:10.1007/s11274-015-1977-0
5. ↑ Kirubakaran SI, Sakthivel N. Cloning and overexpression of antifungal barley chitinase gene in Escherichia coli. Protein Expr Purif. 2007 Mar;52(1):159-66. PMID:17029984 doi:10.1016/j.pep.2006.08.012