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== Function == | == Function == | ||
| - | Trypsin is a serine protease released by the pancreas and secreted into the duodenum that acts as a digestive enzyme that catalyzes the hydrolysis of peptide bonds, specifically for positively charged residues (K, R, H). The catalytic mechanism in which the enzyme acts as a protease is as follows: | + | '''Trypsin''' is a serine protease released by the pancreas and secreted into the duodenum that acts as a digestive enzyme that catalyzes the hydrolysis of peptide bonds, specifically for positively charged residues (K, R, H). The catalytic mechanism in which the enzyme acts as a protease is as follows: |
1. Nucleophillic and base catalysis by enzyme to substrate to form tetrahedral intermediate at carbonyl group of scissile peptide. | 1. Nucleophillic and base catalysis by enzyme to substrate to form tetrahedral intermediate at carbonyl group of scissile peptide. | ||
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5. Acid catalysis by the breaking of the C-O covalent bond of the tetrahedral intermediate, releasing the peptide from the enzyme substrate complex. Once the peptide is released, the enzyme once again becomes active. <ref>PMID:16636277</ref>. | 5. Acid catalysis by the breaking of the C-O covalent bond of the tetrahedral intermediate, releasing the peptide from the enzyme substrate complex. Once the peptide is released, the enzyme once again becomes active. <ref>PMID:16636277</ref>. | ||
| - | Below is a Diagram of the Catalytic Mechanism: | ||
| - | == Disease == | ||
| - | == | + | == Below is a Diagram of the Catalytic Mechanism: == |
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== Structural highlights of Trypsin == | == Structural highlights of Trypsin == | ||
Revision as of 23:19, 18 February 2016
Trypsin Mechanism & Structure - Chase Francoeur, Elias Arellano
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