5b3h
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The crystal structure of the JACKDAW/IDD10 bound to the heterodimeric SHR-SCR complex== |
| + | <StructureSection load='5b3h' size='340' side='right' caption='[[5b3h]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5b3h]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B3H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B3H FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5b3g|5b3g]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b3h OCA], [http://pdbe.org/5b3h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b3h RCSB], [http://www.ebi.ac.uk/pdbsum/5b3h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b3h ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/SCR_ARATH SCR_ARATH]] Transcription factor required for quiescent center cells specification and maintenance of surrounding stem cells, and for the asymmetric cell division involved in radial pattern formation in roots. Essential for cell division but not differentiation of the ground tissue. Also required for normal shoot gravitropism. Regulates the radial organization of the shoot axial organs. Binds to the promoter of MGP, NUC, RLK and SCL3. Restricts SHR movment and sequesters it into the nucleus of the endodermis.<ref>PMID:10631180</ref> <ref>PMID:12569126</ref> <ref>PMID:15142972</ref> <ref>PMID:15314023</ref> <ref>PMID:16640459</ref> <ref>PMID:17446396</ref> <ref>PMID:22921914</ref> <ref>PMID:24302889</ref> <ref>PMID:8819871</ref> <ref>PMID:9375406</ref> <ref>PMID:9670559</ref> [[http://www.uniprot.org/uniprot/IDD10_ARATH IDD10_ARATH]] Transcription factor that regulates tissue boundaries and asymmetric cell division by a rapid up-regulation of 'SCARECROW' (SCR), thus controlling the nuclear localization of 'SHORT-ROOT' (SHR) and restricting its action(PubMed:17785527). Required for radial patterning and stem cell maintenance(PubMed:17785527). Counteracted by 'MAGPIE' (MGP)(PubMed:17785527). Binds to the SCR and MGP promoter sequences (PubMed:21935722). Controls position-dependent signals that regulate epidermal-cell-type patterning (PubMed:20356954).<ref>PMID:17785527</ref> <ref>PMID:20356954</ref> <ref>PMID:21935722</ref> [[http://www.uniprot.org/uniprot/SHR_ARATH SHR_ARATH]] Transcription factor required for quiescent center cells specification and maintenance of surrounding stem cells, and for the asymmetric cell division involved in radial pattern formation in roots. Essential for both cell division and cell specification. Regulates the radial organization of the shoot axial organs and is required for normal shoot gravitropism. Directly controls the transcription of SCR, and when associated with SCR, of MGP, RLK, TRI, NUC and SCL3.<ref>PMID:10850497</ref> <ref>PMID:12569126</ref> <ref>PMID:15314023</ref> <ref>PMID:16640459</ref> <ref>PMID:17446396</ref> <ref>PMID:9670559</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The plant-specific GAI, RGA and SCR (GRAS) family proteins play critical roles in plant development and signalling. Two GRAS proteins, SHORT-ROOT (SHR) and SCARECROW (SCR), cooperatively direct asymmetric cell division and the patterning of root cell types by transcriptional control in conjunction with BIRD/INDETERMINATE DOMAIN (IDD) transcription factors, although precise details of these specific interactions and actions remain unknown. Here, we present the crystal structures of the SHR-SCR binary and JACKDAW (JKD)/IDD10-SHR-SCR ternary complexes. Each GRAS domain comprises one alpha/beta core subdomain with an alpha-helical cap that mediates heterodimerization by forming an intermolecular helix bundle. The alpha/beta core subdomain of SHR forms the BIRD binding groove, which specifically recognizes the zinc fingers of JKD. We identified a conserved SHR-binding motif in 13 BIRD/IDD transcription factors. Our results establish a structural basis for GRAS-GRAS and GRAS-BIRD interactions and provide valuable clues towards our understanding of these regulators, which are involved in plant-specific signalling networks. | ||
| - | + | Structure of the SHR-SCR heterodimer bound to the BIRD/IDD transcriptional factor JKD.,Hirano Y, Nakagawa M, Suyama T, Murase K, Shirakawa M, Takayama S, Sun TP, Hakoshima T Nat Plants. 2017 Feb 17;3:17010. doi: 10.1038/nplants.2017.10. PMID:28211915<ref>PMID:28211915</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5b3h" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Hakoshima, T]] | ||
| + | [[Category: Hirano, Y]] | ||
| + | [[Category: Nakagawa, M]] | ||
| + | [[Category: Suyama, T]] | ||
| + | [[Category: Transcription]] | ||
| + | [[Category: Transcription factor]] | ||
Revision as of 09:30, 11 March 2017
The crystal structure of the JACKDAW/IDD10 bound to the heterodimeric SHR-SCR complex
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