Rhomboid protease

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<StructureSection load='2xow' size='350' side='right' caption='Structure of rhomboid protease complex with inhibitor isocoumarin and nonyglucoside detergent molecules (PDB entry [[2xow]])' scene=''>
<StructureSection load='2xow' size='350' side='right' caption='Structure of rhomboid protease complex with inhibitor isocoumarin and nonyglucoside detergent molecules (PDB entry [[2xow]])' scene=''>
== Function ==
== Function ==
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'''Rhomboid protease''' is a transmembranal serine protease. The active site is buried in the lipid bilayer of cell membrane. It cleaves proteins within their transmembrane domain<ref>PMID:18605900</ref>. Rhomboids regulate many cellular processes and are involved in many human diseases. The catalytic site of rhomboids contains a hydrophilic pocket protected from the lipid bilayer. Rhomboids are specific for a single transmembranal helix proteins.
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'''Rhomboid protease''' (GlpG) is a transmembranal serine protease. The active site is buried in the lipid bilayer of cell membrane. It cleaves proteins within their transmembrane domain<ref>PMID:18605900</ref>. Rhomboids regulate many cellular processes and are involved in many human diseases. The catalytic site of rhomboids contains a hydrophilic pocket protected from the lipid bilayer. Rhomboids are specific for a single transmembranal helix proteins.
== Structural highlights ==
== Structural highlights ==
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**[[2ic8]], [[2irv]], [[2nrf]], [[2o7l]], [[3b45]], [[2xov]], [[4njn]], [[4njp]] – EcGlpG core region – ''Escherichia coli '' <BR />
**[[2ic8]], [[2irv]], [[2nrf]], [[2o7l]], [[3b45]], [[2xov]], [[4njn]], [[4njp]] – EcGlpG core region – ''Escherichia coli '' <BR />
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**[[2xtu]], [[3b44]], [[2xtv]] – EcGlpG core region (mutant) <BR />
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**[[2xtu]], [[3b44]], [[2xtv]], [[5f5d]] – EcGlpG core region (mutant) <BR />
**[[4hdd]] – EcGlpG N terminal <BR />
**[[4hdd]] – EcGlpG N terminal <BR />
**[[2lep]], [[2mja]] – EcGlpG N terminal - NMR <BR />
**[[2lep]], [[2mja]] – EcGlpG N terminal - NMR <BR />
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**[[3ubb]] – EcGlpG core region + phosphonofluoridate inhibitor <BR />
**[[3ubb]] – EcGlpG core region + phosphonofluoridate inhibitor <BR />
**[[3zmh]], [[3zmi]], [[3zmj]], [[3zot]] – EcGlpG core region + β-lactam inhibitor <BR />
**[[3zmh]], [[3zmi]], [[3zmj]], [[3zot]] – EcGlpG core region + β-lactam inhibitor <BR />
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**[[5f5j]], [[5f5k]] – EcGlpG core region (mutant) + aldehyde inhibitor <BR />
**[[4qnz]] – EcGlpG core region (mutant) + peptide inhibitor <BR />
**[[4qnz]] – EcGlpG core region (mutant) + peptide inhibitor <BR />
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**[[4qo0]], [[4qo2]] – EcGlpG core region + peptide inhibitor <BR />
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**[[4qo0]], [[4qo2]], [[5f5g]], [[5f5b]] – EcGlpG core region + peptide inhibitor <BR />
}}
}}

Revision as of 07:51, 10 August 2016

Structure of rhomboid protease complex with inhibitor isocoumarin and nonyglucoside detergent molecules (PDB entry 2xow)

Drag the structure with the mouse to rotate

3D Structures of rhomboid protease

Updated on 10-August-2016

References

  1. Freeman M. Rhomboid proteases and their biological functions. Annu Rev Genet. 2008;42:191-210. doi: 10.1146/annurev.genet.42.110807.091628. PMID:18605900 doi:http://dx.doi.org/10.1146/annurev.genet.42.110807.091628
  2. Vinothkumar KR, Strisovsky K, Andreeva A, Christova Y, Verhelst S, Freeman M. The structural basis for catalysis and substrate specificity of a rhomboid protease. EMBO J. 2010 Oct 1. PMID:20890268 doi:10.1038/emboj.2010.243

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Michal Harel, Alexander Berchansky, Joel L. Sussman

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