CRISPR-Cas9

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<StructureSection load='4qyz' size='450' side='right' scene='74/742625/Cv/4' caption=''>
<StructureSection load='4qyz' size='450' side='right' scene='74/742625/Cv/4' caption=''>
==Crystal structure of a CRISPR RNA-guided surveillance complex, Cascade, bound to a ssDNA target<ref>PMID:25123481</ref>==
==Crystal structure of a CRISPR RNA-guided surveillance complex, Cascade, bound to a ssDNA target<ref>PMID:25123481</ref>==
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The <scene name='74/742625/Cv/3'>crystal structure of ssDNA-bound Cascade has the seahorse architecture</scene>. The body is formed by a helical filament of six Cas7 subunits (Cas7.1 to 7.6) wrapped around the crRNA guide, with a head-to-tail dimer of Cse2 (Cse2.1 and Cse2.2) at the belly. Cas6e and the 3′ handle of crRNA cap the Cas7 filament atthe head while Cas5 and the 5′ handle cap the tail. The N-terminal base of Cse1 is positioned at the tail of the filament; the C-terminal four-helix bundle contacts Cse2.2. The ssDNAtarget is juxtaposed to the guide region of the crRNA in a groove formed by the Cas7 filament, the four-helix bundle of Cse1, and the Cse2 dimer.
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The <scene name='74/742625/Cv/5'>crystal structure of ssDNA-bound Cascade has the seahorse architecture</scene>. The body is formed by a helical filament of six Cas7 subunits (Cas7.1 to 7.6) wrapped around the crRNA guide, with a head-to-tail dimer of Cse2 (Cse2.1 and Cse2.2) at the belly. Cas6e and the 3′ handle of crRNA cap the Cas7 filament atthe head while Cas5 and the 5′ handle cap the tail. The N-terminal base of Cse1 is positioned at the tail of the filament; the C-terminal four-helix bundle contacts Cse2.2. The ssDNA target is juxtaposed to the guide region of the crRNA in a groove formed by the Cas7 filament, the four-helix bundle of Cse1, and the Cse2 dimer.
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*<scene name='74/742625/Cv/6'>90° rotation about axis Z</scene>.
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*<scene name='74/742625/Cv/7'>90° rotation about axis Y</scene>
==Crystal structure of the E. coli CRISPR RNA-guided surveillance complex, Cascade<ref>PMID:25103409</ref>==
==Crystal structure of the E. coli CRISPR RNA-guided surveillance complex, Cascade<ref>PMID:25103409</ref>==
</StructureSection>
</StructureSection>
== References ==
== References ==
<references/>
<references/>

Revision as of 14:02, 20 September 2016

PDB ID 4qyz

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References

  1. ↑ Mulepati S, Heroux A, Bailey S. Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target. Science. 2014 Aug 14. pii: 1256996. PMID:25123481 doi:http://dx.doi.org/10.1126/science.1256996
  2. ↑ Jackson RN, Golden SM, van Erp PB, Carter J, Westra ER, Brouns SJ, van der Oost J, Terwilliger TC, Read RJ, Wiedenheft B. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Science. 2014 Aug 7. pii: 1256328. PMID:25103409 doi:http://dx.doi.org/10.1126/science.1256328

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Alexander Berchansky, Michal Harel

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