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2x4n

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 ==Crystal structure of MHC CLass I HLA-A2.1 bound to residual fragments of a photocleavable peptide that is cleaved upon UV-light treatment==
-<StructureSection load='2x4n' size='340' side='right' caption='[[2x4n]], [[Resolution|resolution]] 2.34&Aring;' scene=''>
+<StructureSection load='2x4n' size='340' side='right'caption='[[2x4n]], [[Resolution|resolution]] 2.34&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2x4n]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X4N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X4N FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2x4n]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X4N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2X4N FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PRQ:(3S)-3-AMINO-3-(2-NITROPHENYL)PROPANOIC+ACID'>PRQ</scene>, <scene name='pdbligand=PRV:(2R)-AMINO(2-NITROPHENYL)ETHANOIC+ACID'>PRV</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1uqs|1uqs]], [[1bd2|1bd2]], [[2esv|2esv]], [[2ak4|2ak4]], [[1ypz|1ypz]], [[1im3|1im3]], [[1uxw|1uxw]], [[1i7u|1i7u]], [[1c16|1c16]], [[1hsa|1hsa]], [[2axf|2axf]], [[1gzp|1gzp]], [[2bnq|2bnq]], [[1w72|1w72]], [[2jcc|2jcc]], [[2bck|2bck]], [[1de4|1de4]], [[2vlk|2vlk]], [[1exu|1exu]], [[1qrn|1qrn]], [[2hla|2hla]], [[1mhe|1mhe]], [[1im9|1im9]], [[1eez|1eez]], [[1jht|1jht]], [[1qqd|1qqd]], [[1qr1|1qr1]], [[1zs8|1zs8]], [[1hla|1hla]], [[1jgd|1jgd]], [[1i1y|1i1y]], [[1vgk|1vgk]], [[1age|1age]], [[1ur7|1ur7]], [[1hhg|1hhg]], [[1s9x|1s9x]], [[1a9e|1a9e]], [[1duz|1duz]], [[2x4u|2x4u]], [[2clr|2clr]], [[3hla|3hla]], [[1m05|1m05]], [[2x4o|2x4o]], [[1tvb|1tvb]], [[2v2w|2v2w]], [[1onq|1onq]], [[2vlr|2vlr]], [[2x4p|2x4p]], [[2bvo|2bvo]], [[1a1n|1a1n]], [[1lp9|1lp9]], [[1zsd|1zsd]], [[1m6o|1m6o]], [[1hhk|1hhk]], [[1hsb|1hsb]], [[1zt4|1zt4]], [[1ce6|1ce6]], [[1x7q|1x7q]], [[1py4|1py4]], [[1syv|1syv]], [[2j8u|2j8u]], [[1sys|1sys]], [[1ogt|1ogt]], [[2x4t|2x4t]], [[1cg9|1cg9]], [[1p7q|1p7q]], [[1q94|1q94]], [[1jnj|1jnj]], [[1agb|1agb]], [[2d31|2d31]], [[1aqd|1aqd]], [[1xz0|1xz0]], [[1lds|1lds]], [[1tvh|1tvh]], [[1hhh|1hhh]], [[1xr8|1xr8]], [[2bss|2bss]], [[1e28|1e28]], [[1a1m|1a1m]], [[2bvp|2bvp]], [[2v2x|2v2x]], [[1xr9|1xr9]], [[2gj6|2gj6]], [[2x4r|2x4r]], [[1efx|1efx]], [[1qlf|1qlf]], [[2av1|2av1]], [[1tmc|1tmc]], [[1qsf|1qsf]], [[1duy|1duy]], [[1kpr|1kpr]], [[1jge|1jge]], [[2hjl|2hjl]], [[1qew|1qew]], [[1w0v|1w0v]], [[1k5n|1k5n]], [[1ao7|1ao7]], [[2bnr|2bnr]], [[1xh3|1xh3]], [[2bst|2bst]], [[1mi5|1mi5]], [[2h26|2h26]], [[1s9y|1s9y]], [[1a1o|1a1o]], [[2a83|2a83]], [[1agf|1agf]], [[1oga|1oga]], [[2f8o|2f8o]], [[2cii|2cii]], [[1i7r|1i7r]], [[1jf1|1jf1]], [[2c7u|2c7u]], [[2f74|2f74]], [[1e27|1e27]], [[1w0w|1w0w]], [[1gzq|1gzq]], [[1uxs|1uxs]], [[1akj|1akj]], [[2hjk|2hjk]], [[2vb5|2vb5]], [[1agd|1agd]], [[1r3h|1r3h]], [[1eey|1eey]], [[1i7t|1i7t]], [[1ydp|1ydp]], [[1i4f|1i4f]], [[2vll|2vll]], [[2bsr|2bsr]], [[1of2|1of2]], [[2vlj|2vlj]], [[2x4s|2x4s]], [[1b0r|1b0r]], [[1b0g|1b0g]], [[1hhi|1hhi]], [[1qse|1qse]], [[2axg|2axg]], [[1a9b|1a9b]], [[2bvq|2bvq]], [[1agc|1agc]], [[1qvo|1qvo]], [[1hhj|1hhj]], [[1s9w|1s9w]], [[1ktl|1ktl]], [[1a6z|1a6z]], [[2cik|2cik]], [[2uwe|2uwe]], [[1i1f|1i1f]], [[2av7|2av7]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x4n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x4n OCA], [http://pdbe.org/2x4n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x4n RCSB], [http://www.ebi.ac.uk/pdbsum/2x4n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x4n ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2x4n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x4n OCA], [http://pdbe.org/2x4n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x4n RCSB], [http://www.ebi.ac.uk/pdbsum/2x4n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x4n ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 32: / Line 32: @@
 ==See Also==
-*[[Beta-2 microglobulin|Beta-2 microglobulin]]
+*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
-*[[Major histocompatibility complex|Major histocompatibility complex]]
+*[[MHC 3D structures of MHC|MHC 3D structures of MHC]]
 == References ==
 <references/>
@@ Line 39: / Line 39: @@
 </StructureSection>
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Celie, P H.N]]
 [[Category: Ovaa, H]]

Revision as of 15:04, 8 July 2020

Crystal structure of MHC CLass I HLA-A2.1 bound to residual fragments of a photocleavable peptide that is cleaved upon UV-light treatment

spinqualitylabelsall models

PDB ID 2x4n

Show:Asymmetric Unit Biological Assembly

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Structural highlights

2x4n is a 6 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:	,
Related:	1uqs, 1bd2, 2esv, 2ak4, 1ypz, 1im3, 1uxw, 1i7u, 1c16, 1hsa, 2axf, 1gzp, 2bnq, 1w72, 2jcc, 2bck, 1de4, 2vlk, 1exu, 1qrn, 2hla, 1mhe, 1im9, 1eez, 1jht, 1qqd, 1qr1, 1zs8, 1hla, 1jgd, 1i1y, 1vgk, 1age, 1ur7, 1hhg, 1s9x, 1a9e, 1duz, 2x4u, 2clr, 3hla, 1m05, 2x4o, 1tvb, 2v2w, 1onq, 2vlr, 2x4p, 2bvo, 1a1n, 1lp9, 1zsd, 1m6o, 1hhk, 1hsb, 1zt4, 1ce6, 1x7q, 1py4, 1syv, 2j8u, 1sys, 1ogt, 2x4t, 1cg9, 1p7q, 1q94, 1jnj, 1agb, 2d31, 1aqd, 1xz0, 1lds, 1tvh, 1hhh, 1xr8, 2bss, 1e28, 1a1m, 2bvp, 2v2x, 1xr9, 2gj6, 2x4r, 1efx, 1qlf, 2av1, 1tmc, 1qsf, 1duy, 1kpr, 1jge, 2hjl, 1qew, 1w0v, 1k5n, 1ao7, 2bnr, 1xh3, 2bst, 1mi5, 2h26, 1s9y, 1a1o, 2a83, 1agf, 1oga, 2f8o, 2cii, 1i7r, 1jf1, 2c7u, 2f74, 1e27, 1w0w, 1gzq, 1uxs, 1akj, 2hjk, 2vb5, 1agd, 1r3h, 1eey, 1i7t, 1ydp, 1i4f, 2vll, 2bsr, 1of2, 2vlj, 2x4s, 1b0r, 1b0g, 1hhi, 1qse, 2axg, 1a9b, 2bvq, 1agc, 1qvo, 1hhj, 1s9w, 1ktl, 1a6z, 2cik, 2uwe, 1i1f, 2av7
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[1A02_HUMAN] Involved in the presentation of foreign antigens to the immune system.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

High-throughput structure determination of protein-ligand complexes is central in drug development and structural proteomics. To facilitate such high-throughput structure determination we designed an induced replacement strategy. Crystals of a protein complex bound to a photosensitive ligand are exposed to UV light, inducing the departure of the bound ligand, allowing a new ligand to soak in. We exemplify the approach for a class of protein complexes that is especially recalcitrant to high-throughput strategies: the MHC class I proteins. We developed a UV-sensitive, "conditional", peptide ligand whose UV-induced cleavage in the crystals leads to the exchange of the low-affinity lytic fragments for full-length peptides introduced in the crystallant solution. This "in crystallo" exchange is monitored by the loss of seleno-methionine anomalous diffraction signal of the conditional peptide compared to the signal of labeled MHC beta2m subunit. This method has the potential to facilitate high-throughput crystallography in various protein families.

UV-induced ligand exchange in MHC class I protein crystals.,Celie PH, Toebes M, Rodenko B, Ovaa H, Perrakis A, Schumacher TN J Am Chem Soc. 2009 Sep 2;131(34):12298-304. PMID:19655750^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Celie PH, Toebes M, Rodenko B, Ovaa H, Perrakis A, Schumacher TN. UV-induced ligand exchange in MHC class I protein crystals. J Am Chem Soc. 2009 Sep 2;131(34):12298-304. PMID:19655750 doi:10.1021/ja9037559

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

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2x4n

From Proteopedia

Revision as of 15:04, 8 July 2020

Crystal structure of MHC CLass I HLA-A2.1 bound to residual fragments of a photocleavable peptide that is cleaved upon UV-light treatment

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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