5epo

From Proteopedia

(Difference between revisions)

Revision as of 08:14, 12 July 2023

The three-dimensional structure of Clostridium absonum 7alpha-hydroxysteroid dehydrogenase

Structural highlights

5epo is a 4 chain structure with sequence from Clostridium sardiniense. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HDHA_CLOSR 7alpha-hydroxysteroid dehydrogenase that catalyzes the NADP(+)-dependent oxidation of the 7alpha-hydroxy group of 7alpha-hydroxysteroids, such as cholate, chenodeoxycholate, glycochenodeoxycholate and taurochenodeoxycholate, to the corresponding 7-oxosteroids (PubMed:22198717, PubMed:24810359). Is also able to catalyze the reverse reduction reactions (PubMed:22198717). Together with 7beta-HSDH encoded in the adjacent gene, is likely involved in the epimerization of the hydroxy group at C-7 of primary bile acids through 7-keto bile acid intermediates (PubMed:22198717).^[1] ^[2]

Publication Abstract from PubMed

7alpha-hydroxysteroid dehydrogenase (7alpha-HSDH) can catalyse the oxidation of C7 alpha-OH of the steroid nucleus in the bile acid metabolism. In the paper we determined the crystal structure of 7alpha-HSDH from Clostridium absonum (CA 7alpha-HSDH) complexed with taurochenodeoxycholic acid (TCDCA) and NADP(+) by X-ray diffraction, which, as a tetramer, possesses the typical alpha/beta folding pattern. The four subunits of an asymmetric unit lie in the fact that there are the stable hydrophobic interactions between Q-axis-related subunits. Significantly, we captured an active state of the NADP(+), confirming that nicotinamide moiety of NADP(+) act as electron carrier in the dehydrogenation. On the basis of crystal structure analysis, site-directed mutagenesis and MD simulation, furthermore, we find that the guanidinium of Arg38 can form the stable cation-pi interaction with the adenine ring of NADP(+), and the cation-pi interaction and hydrogen bonds between Arg38 and NADP(+) have a significant anchor effect on the cofactor binding to CA 7alpha-HSDH.

The three-dimensional structure of Clostridium absonum 7alpha-hydroxysteroid dehydrogenase: new insights into the conserved arginines for NADP(H) recognition.,Lou D, Wang B, Tan J, Zhu L, Cen X, Ji Q, Wang Y Sci Rep. 2016 Mar 10;6:22885. doi: 10.1038/srep22885. PMID:26961171^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ferrandi EE, Bertolesi GM, Polentini F, Negri A, Riva S, Monti D. In search of sustainable chemical processes: cloning, recombinant expression, and Appl Microbiol Biotechnol. 2012 Sep;95(5):1221-33. PMID:22198717 doi:10.1007/s00253-011-3798-x
↑ Lou D, Wang B, Tan J, Zhu L. Carboxyl-terminal and Arg38 are essential for activity of the 7α-hydroxysteroid dehydrogenase from Clostridium absonum. Protein Pept Lett. 2014;21(9):894-900. PMID:24810359 doi:10.2174/0929866521666140507160050
↑ Lou D, Wang B, Tan J, Zhu L, Cen X, Ji Q, Wang Y. The three-dimensional structure of Clostridium absonum 7alpha-hydroxysteroid dehydrogenase: new insights into the conserved arginines for NADP(H) recognition. Sci Rep. 2016 Mar 10;6:22885. doi: 10.1038/srep22885. PMID:26961171 doi:http://dx.doi.org/10.1038/srep22885

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5epo"

Categories: Clostridium sardiniense | Large Structures | Lou D | Wang B | Wang F

@@ Line 3: / Line 3: @@
 <StructureSection load='5epo' size='340' side='right'caption='[[5epo]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5epo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EPO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5EPO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5epo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_sardiniense Clostridium sardiniense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EPO FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=TUD:TAUROCHENODEOXYCHOLIC+ACID'>TUD</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5epo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5epo OCA], [http://pdbe.org/5epo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5epo RCSB], [http://www.ebi.ac.uk/pdbsum/5epo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5epo ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=TUD:TAUROCHENODEOXYCHOLIC+ACID'>TUD</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5epo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5epo OCA], [https://pdbe.org/5epo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5epo RCSB], [https://www.ebi.ac.uk/pdbsum/5epo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5epo ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/HDHA_CLOSR HDHA_CLOSR] 7alpha-hydroxysteroid dehydrogenase that catalyzes the NADP(+)-dependent oxidation of the 7alpha-hydroxy group of 7alpha-hydroxysteroids, such as cholate, chenodeoxycholate, glycochenodeoxycholate and taurochenodeoxycholate, to the corresponding 7-oxosteroids (PubMed:22198717, PubMed:24810359). Is also able to catalyze the reverse reduction reactions (PubMed:22198717). Together with 7beta-HSDH encoded in the adjacent gene, is likely involved in the epimerization of the hydroxy group at C-7 of primary bile acids through 7-keto bile acid intermediates (PubMed:22198717).<ref>PMID:22198717</ref> <ref>PMID:24810359</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 16: / Line 19: @@
 </div>
 <div class="pdbe-citations 5epo" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Hydroxysteroid dehydrogenase 3D structures|Hydroxysteroid dehydrogenase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Clostridium sardiniense]]
 [[Category: Large Structures]]
-[[Category: Lou, D]]
+[[Category: Lou D]]
-[[Category: Wang, B]]
+[[Category: Wang B]]
-[[Category: Wang, F]]
+[[Category: Wang F]]
-[[Category: Metabolic process]]
-[[Category: Oxidoreductase]]
-[[Category: Oxidoreductase activity]]

5epo

From Proteopedia

Revision as of 08:14, 12 July 2023

The three-dimensional structure of Clostridium absonum 7alpha-hydroxysteroid dehydrogenase

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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