6yug

<table><tr><td colspan='2'>[[6yug]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Crypi Crypi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YUG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YUG FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=G6P:ALPHA-D-GLUCOSE-6-PHOSPHATE'>G6P</scene></td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSAT, cgd4_4000 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=353152 CRYPI])</td></tr>

<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] </span></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yug OCA], [http://pdbe.org/6yug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yug RCSB], [http://www.ebi.ac.uk/pdbsum/6yug PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yug ProSAT]</span></td></tr>

== Function ==

[[http://www.uniprot.org/uniprot/SSAT_CRYPI SSAT_CRYPI]] Enzyme which catalyzes the acetylation of polyamines. Displays higher substrate specificity for spermine than for spermidine. May function to acetylate host-derived polyamines, thus alleviating the necessity for de novo synthesis of these molecules.<ref>PMID:17289169</ref> <ref>PMID:17289169</ref>

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== Publication Abstract from PubMed ==

UDP-N-acetylglucosamine (UDP-GlcNAc), the main product of the hexosamine biosynthetic pathway, is an important metabolite in protozoan parasites since its sugar moiety is incorporated into glycosylphosphatidylinositol (GPI) glycolipids and N- and O-linked glycans. Apicomplexan parasites have a hexosamine pathway comparable to other eukaryotic organisms, with the exception of the glucosamine-phosphate N-acetyltransferase (GNA1) enzymatic step that has an independent evolutionary origin and significant differences from nonapicomplexan GNA1s. By using conditional genetic engineering, we demonstrate the requirement of GNA1 for the generation of a pool of UDP-GlcNAc and for the development of intraerythrocytic asexual Plasmodium falciparum parasites. Furthermore, we present the 1.95 A resolution structure of the GNA1 ortholog from Cryptosporidium parvum, an apicomplexan parasite which is a leading cause of diarrhea in developing countries, as a surrogate for P. falciparum GNA1. The in-depth analysis of the crystal shows the presence of specific residues relevant for GNA1 enzymatic activity that are further investigated by the creation of site-specific mutants. The experiments reveal distinct features in apicomplexan GNA1 enzymes that could be exploitable for the generation of selective inhibitors against these parasites, by targeting the hexosamine pathway. This work underscores the potential of apicomplexan GNA1 as a drug target against malaria.IMPORTANCE Apicomplexan parasites cause a major burden on global health and economy. The absence of treatments, the emergence of resistances against available therapies, and the parasite's ability to manipulate host cells and evade immune systems highlight the urgent need to characterize new drug targets to treat infections caused by these parasites. We demonstrate that glucosamine-6-phosphate N-acetyltransferase (GNA1), required for the biosynthesis of UDP-N-acetylglucosamine (UDP-GlcNAc), is essential for P. falciparum asexual blood stage development and that the disruption of the gene encoding this enzyme quickly causes the death of the parasite within a life cycle. The high-resolution crystal structure of the GNA1 ortholog from the apicomplexan parasite C. parvum, used here as a surrogate, highlights significant differences from human GNA1. These divergences can be exploited for the design of specific inhibitors against the malaria parasite.

 

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== References ==

<references/>

[[Category: Crypi]]

[[Category: Diamine N-acetyltransferase]]

[[Category: Borges, R]]

[[Category: Chi, J]]

[[Category: Cova, M]]

[[Category: Hurtado-Guerrero, R]]

[[Category: Izquierdo, L]]

[[Category: Leivar, P]]

[[Category: Medina, A]]

[[Category: Planas, A]]

[[Category: Rivas, M de las]]

[[Category: Uson, I]]

[[Category: Acyltransferase]]

[[Category: Transferase]]