7mpy
From Proteopedia
(Difference between revisions)
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==Crystal structure of cytosolic HPPK-DHPS from A.thaliana== | ==Crystal structure of cytosolic HPPK-DHPS from A.thaliana== | ||
| - | <StructureSection load='7mpy' size='340' side='right'caption='[[7mpy]]' scene=''> | + | <StructureSection load='7mpy' size='340' side='right'caption='[[7mpy]], [[Resolution|resolution]] 2.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MPY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7mpy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7MPY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7MPY FirstGlance]. <br> |
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mpy OCA], [https://pdbe.org/7mpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mpy RCSB], [https://www.ebi.ac.uk/pdbsum/7mpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mpy ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7mpy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7mpy OCA], [https://pdbe.org/7mpy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7mpy RCSB], [https://www.ebi.ac.uk/pdbsum/7mpy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7mpy ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/FOLC_ARATH FOLC_ARATH]] Catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis (PubMed:17289662). Plays a role in seed stress response and survival (PubMed:17289662, PubMed:21996493).<ref>PMID:17289662</ref> <ref>PMID:21996493</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Herbicides are vital for modern agriculture, but their utility is threatened by genetic or metabolic resistance in weeds, as well as regulatory barriers. Of the known herbicide modes of action, 7,8-dihydropterin synthase (DHPS), which is involved in folate biosynthesis, is targeted by just one commercial herbicide, asulam. A mimic of the substrate para-aminobenzoic acid, asulam is chemically similar to sulfonamide antibiotics, and although it is still in widespread use, asulam has faced regulatory scrutiny. With an entire mode of action represented by just one commercial agrochemical, we sought to improve the understanding of its plant target. Here we solve a 2.3 A resolution crystal structure for Arabidopsis thaliana DHPS that is conjoined to 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK), and we reveal a strong structural conservation with bacterial counterparts at the sulfonamide-binding pocket of DHPS. We demonstrate that asulam and the antibiotic sulfamethoxazole have herbicidal as well as antibacterial activity, and we explore the structural basis of their potency by modeling these compounds in mitochondrial HPPK/DHPS. Our findings suggest limited opportunity for the rational design of plant selectivity from asulam and indicate that pharmacokinetic or delivery differences between plants and microbes might be the best ways to safeguard this mode of action. | ||
| + | |||
| + | Crystal structure of Arabidopsis thaliana HPPK/DHPS, a bifunctional enzyme and target of the herbicide asulam.,Vadlamani G, Sukhoverkov KV, Haywood J, Breese KJ, Fisher MF, Stubbs KA, Bond CS, Mylne JS Plant Commun. 2022 Apr 9:100322. doi: 10.1016/j.xplc.2022.100322. PMID:35605193<ref>PMID:35605193</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7mpy" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Bond | + | [[Category: Bond, C S]] |
| - | [[Category: Vadlamani G]] | + | [[Category: Vadlamani, G]] |
| + | [[Category: Folate biosynthesis]] | ||
| + | [[Category: Herbicide target]] | ||
| + | [[Category: Plant protein]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 05:35, 8 June 2022
Crystal structure of cytosolic HPPK-DHPS from A.thaliana
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