4ady

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of 26S proteasome subunit Rpn2

Structural highlights

4ady is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.7Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RPN2_YEAST Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.^[1]

Publication Abstract from PubMed

The 26S proteasome proteolyses ubiquitylated proteins and is assembled from a 20S proteolytic core and two 19S regulatory particles (19S-RP). The 19S-RP scaffolding subunits Rpn1 and Rpn2 function to engage ubiquitin receptors. Rpn1 and Rpn2 are characterized by eleven tandem copies of a 35-40 amino acid repeat motif termed the proteasome/cyclosome (PC) repeat. Here, we reveal that the eleven PC repeats of Rpn2 form a closed toroidal structure incorporating two concentric rings of alpha helices encircling two axial alpha helices. A rod-like N-terminal domain consisting of 17 stacked alpha helices and a globular C-terminal domain emerge from one face of the toroid. Rpn13, an ubiquitin receptor, binds to the C-terminal 20 residues of Rpn2. Rpn1 adopts a similar conformation to Rpn2 but differs in the orientation of its rod-like N-terminal domain. These findings have implications for understanding how 19S-RPs recognize, unfold, and deliver ubiquitylated substrates to the 20S core.

The Structure of the 26S Proteasome Subunit Rpn2 Reveals Its PC Repeat Domain as a Closed Toroid of Two Concentric alpha-Helical Rings.,He J, Kulkarni K, da Fonseca PC, Krutauz D, Glickman MH, Barford D, Morris EP Structure. 2012 Mar 7;20(3):513-21. PMID:22405010^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Glickman MH, Rubin DM, Fried VA, Finley D. The regulatory particle of the Saccharomyces cerevisiae proteasome. Mol Cell Biol. 1998 Jun;18(6):3149-62. PMID:9584156
↑ He J, Kulkarni K, da Fonseca PC, Krutauz D, Glickman MH, Barford D, Morris EP. The Structure of the 26S Proteasome Subunit Rpn2 Reveals Its PC Repeat Domain as a Closed Toroid of Two Concentric alpha-Helical Rings. Structure. 2012 Mar 7;20(3):513-21. PMID:22405010 doi:10.1016/j.str.2011.12.015

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ady"

@@ Line 3: / Line 3: @@
 <StructureSection load='4ady' size='340' side='right'caption='[[4ady]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ady]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ADY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ADY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ady]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ADY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ADY FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ady FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ady OCA], [https://pdbe.org/4ady PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ady RCSB], [https://www.ebi.ac.uk/pdbsum/4ady PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ady ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/RPN2_YEAST RPN2_YEAST]] Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.<ref>PMID:9584156</ref>
+[https://www.uniprot.org/uniprot/RPN2_YEAST RPN2_YEAST] Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.<ref>PMID:9584156</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 18824]]
 [[Category: Large Structures]]
-[[Category: Barford, D]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Fonseca, P C.A Da]]
+[[Category: Barford D]]
-[[Category: Glickman, M H]]
+[[Category: Da Fonseca PCA]]
-[[Category: He, J]]
+[[Category: Glickman MH]]
-[[Category: Krutauz, D]]
+[[Category: He J]]
-[[Category: Kulkarni, K]]
+[[Category: Krutauz D]]
-[[Category: Morris, E P]]
+[[Category: Kulkarni K]]
-[[Category: Pc repeat]]
+[[Category: Morris EP]]
-[[Category: Protein binding]]
-[[Category: Rpn1]]

4ady

From Proteopedia

Current revision

Crystal structure of 26S proteasome subunit Rpn2

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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