BASIL2023GVQ8DN35

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== Function ==
== Function ==
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Through computational docking results, the predicted function for the Q8DN35 protein is that of a glucokinase.
== Binding site ==
== Binding site ==

Revision as of 15:18, 30 March 2023

Q8DN35 Protein

This is a default text for your page BASIL2023GVQ8DN35. Click above on edit this page to modify. Be careful with the < and > signs. You may include any references to papers as in: the use of JSmol in Proteopedia [1] or to the article describing Jmol [2] to the rescue.

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Contents

Crystal structure

Function

Through computational docking results, the predicted function for the Q8DN35 protein is that of a glucokinase.

Binding site

Image:Docking site Q8DN35.png

This image displays potential binding sites to the Q8DN35 protein.

Protein purification analysis

Through the use of a gravity purification column and Ni-NTA resin, Q8DN35 was purified and a Bradford analysis was conducted to determine the amount of protein in the purified sample. This purification resulted in 0.055 mg/mL of protein being purified.

Kinase activity analysis

To observe Q8DN35's activity, a kinase activity was ran with 2 substrates to observe potential substrate usage. The 2 substrates that were tested are NAG and F-6-P.


References

  1. Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
  2. Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644

Proteopedia Page Contributors and Editors (what is this?)

Lucas Kramer, Anie Rehbein, Jaime Prilusky, Bonnie Hall

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