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1u6p

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[[Image:1u6p.gif|left|200px]]
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[[Image:1u6p.png|left|200px]]
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{{STRUCTURE_1u6p| PDB=1u6p | SCENE= }}
{{STRUCTURE_1u6p| PDB=1u6p | SCENE= }}
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'''NMR Structure of the MLV encapsidation signal bound to the Nucleocapsid protein'''
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===NMR Structure of the MLV encapsidation signal bound to the Nucleocapsid protein===
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==Overview==
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All retroviruses specifically package two copies of their genomes during virus assembly, a requirement for strand-transfer-mediated recombination during reverse transcription. Genomic RNA exists in virions as dimers, and the overlap of RNA elements that promote dimerization and encapsidation suggests that these processes may be coupled. Both processes are mediated by the nucleocapsid domain (NC) of the retroviral Gag polyprotein. Here we show that dimerization-induced register shifts in base pairing within the Psi-RNA packaging signal of Moloney murine leukaemia virus (MoMuLV) expose conserved UCUG elements that bind NC with high affinity (dissociation constant = 75 +/- 12 nM). These elements are base-paired and do not bind NC in the monomeric RNA. The structure of the NC complex with a 101-nucleotide 'core encapsidation' segment of the MoMuLV Psi site reveals a network of interactions that promote sequence- and structure-specific binding by NC's single CCHC zinc knuckle. Our findings support a structural RNA switch mechanism for genome encapsidation, in which protein binding sites are sequestered by base pairing in the monomeric RNA and become exposed upon dimerization to promote packaging of a diploid genome.
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The line below this paragraph, {{ABSTRACT_PUBMED_15457265}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15457265 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15457265}}
==About this Structure==
==About this Structure==
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1U6P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6P OCA].
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1U6P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6P OCA].
==Reference==
==Reference==
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[[Category: U-u mismatch]]
[[Category: U-u mismatch]]
[[Category: Zinc finger]]
[[Category: Zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:49:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:21:37 2008''

Revision as of 17:21, 27 July 2008

Image:1u6p.png

Template:STRUCTURE 1u6p

NMR Structure of the MLV encapsidation signal bound to the Nucleocapsid protein

Template:ABSTRACT PUBMED 15457265

About this Structure

1U6P is a Single protein structure of sequence from Moloney murine leukemia virus. Full experimental information is available from OCA.

Reference

Structural basis for packaging the dimeric genome of Moloney murine leukaemia virus., D'Souza V, Summers MF, Nature. 2004 Sep 30;431(7008):586-90. PMID:15457265

Page seeded by OCA on Sun Jul 27 20:21:37 2008

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