Sandbox Eric Martz
From Proteopedia
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This article is under collaborative development by [[User:Eric Martz|Eric Martz]] (UMass) and [http://www.its.caltech.edu/~rjdlab/ Raymond J. Deshaies] and his team (CalTech). <font color='red'>Please do not edit this page unless you are a member of the development team</font>. Once completed, this page may be moved to a permanent article title. | This article is under collaborative development by [[User:Eric Martz|Eric Martz]] (UMass) and [http://www.its.caltech.edu/~rjdlab/ Raymond J. Deshaies] and his team (CalTech). <font color='red'>Please do not edit this page unless you are a member of the development team</font>. Once completed, this page may be moved to a permanent article title. | ||
| - | Proposed article title: <big><b>Release of RING from Cullin by NEDD8ylation</b></big> | + | Proposed article title: <big><b>Release of |
| + | <font color='#a04090'>RING</font> | ||
| + | from | ||
| + | <font color='#b0a090'>Cullin</font> | ||
| + | by | ||
| + | <font color='#a03000'>NEDD8ylation</font></b></big> | ||
<applet load='3dpl_3dqv_acr_morph.pdb' size='500' frame='true' align='right' caption='NEDD8ylation of Cullin-RING' | <applet load='3dpl_3dqv_acr_morph.pdb' size='500' frame='true' align='right' caption='NEDD8ylation of Cullin-RING' | ||
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<jmolButton> | <jmolButton> | ||
| - | <script>spin off; anim off; frame 1;</script> | + | <script>set background model none; spin off; anim off; frame 1;</script> |
<text>Dimer</text> | <text>Dimer</text> | ||
</jmolButton> | </jmolButton> | ||
<jmolButton> | <jmolButton> | ||
| - | <script>spin off; anim off; frame 7-8;</script> | + | <script>set background model none; spin off; anim off; frame 7-8;</script> |
<text>Trimer</text> | <text>Trimer</text> | ||
</jmolButton> | </jmolButton> | ||
Revision as of 04:47, 22 February 2009
This article is under collaborative development by Eric Martz (UMass) and Raymond J. Deshaies and his team (CalTech). Please do not edit this page unless you are a member of the development team. Once completed, this page may be moved to a permanent article title.
Proposed article title: Release of RING from Cullin by NEDD8ylation
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At right is a morph [1] from a Cullin-RING ligase complex (3dpl) to the same complex after NEDD8ylation (covalent addition of the NEDD8 ubiquitin-like chain to LysXXX of Cullin; 3dqv). NEDD8 binds and rotates the C-terminal domain of Cullin, releasing the folded RING domain from its Cullin-binding site. RING remains attached to Cullin by the RING N-terminus which remains embedded in Cullin.
Notes and References
- ↑ The 9-model morph file can be downloaded from Image:3dpl 3dqv acr morph.pdb. The morph represents the change from 3dpl to 3dqv, and only chains C and R which are common to these two models. Only one trimer was used from 3dqv (which contains 2 trimers in the asymmetric unit), namely, the one with chains named A, C, and R. This trimer was obtained from PQS as 3dqv_1.mmol. The morph was performed by the Yale Morph Server (beta server), ID b899128-21508. This produced 8 models numbered 0-7. In the product morph, the orientation of the final chains was not changed. Hence, chain A (NEDD8) from 3dqv.pdb was added as model number 8 (9th model) without the need for special alignment. Morph done by Eric Martz at the request of Raymond J. Deshaies of CalTech.
