Sandbox Eric Martz
From Proteopedia
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</jmol> | </jmol> | ||
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Color traces by chain: | Color traces by chain: | ||
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<jmolButton> | <jmolButton> | ||
| - | <script>select *a; color trace [xa03000];</script> | + | <script>select *a; spacefill off; trace 0.5; color trace [xa03000];</script> |
<text>NEDD8 (A)</text> | <text>NEDD8 (A)</text> | ||
</jmolButton> | </jmolButton> | ||
<jmolButton> | <jmolButton> | ||
| - | <script>select *c; color trace [xb08090];</script> | + | <script>select *c; spacefill off; trace 0.5; color trace [xb08090];</script> |
<text>Cullin (C)</text> | <text>Cullin (C)</text> | ||
</jmolButton> | </jmolButton> | ||
<jmolButton> | <jmolButton> | ||
| - | <script>select * | + | <script>select *r; spacefill off; trace 0.5; color trace [xa04090];</script> |
<text>RING (R)</text> | <text>RING (R)</text> | ||
</jmolButton> | </jmolButton> | ||
</jmol> | </jmol> | ||
| - | + | <center> | |
| - | + | <b><font color='#a04090'>RING (A)</font> | |
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| + | <font color='#b0a090'>Cullin (C)</font> | ||
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| + | <font color='#a03000'>NEDD8</font></b> | ||
| + | </center> | ||
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<jmol> | <jmol> | ||
Revision as of 18:37, 22 February 2009
This article is under collaborative development by Eric Martz (UMass) and Raymond J. Deshaies and his team (CalTech). Please do not edit this page unless you are a member of the development team. Once completed, this page may be moved to a permanent article title.
Proposed article title: Release of RING from Cullin by NEDD8ylation
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Display chains as smoothed backbone traces colored by N->C Rainbow:
| N | C |
Color traces by chain:
RING (A) Cullin (C) NEDD8
At right is a morph
[1]
from a Cullin-RING ligase complex (3dpl) to the same complex after NEDD8ylation (covalent addition of the NEDD8 ubiquitin-like chain to LysXXX of Cullin; 3dqv). NEDD8 binds and rotates the C-terminal domain of Cullin, releasing the folded RING domain from its Cullin-binding site. RING remains attached to Cullin by the RING N-terminus which remains embedded in Cullin.
Notes and References
- ↑ The 9-model morph file can be downloaded from Image:3dpl 3dqv acr morph.pdb. The morph represents the change from 3dpl to 3dqv, and only chains C and R which are common to these two models. Only one trimer was used from 3dqv (which contains 2 trimers in the asymmetric unit), namely, the one with chains named A, C, and R. This trimer was obtained from PQS as 3dqv_1.mmol. The morph was performed by the Yale Morph Server (beta server), ID b899128-21508. This produced 8 models numbered 0-7. In the product morph, the orientation of the final chains was not changed. Hence, chain A (NEDD8) from 3dqv.pdb was added as model number 8 (9th model) without the need for special alignment. Morph done by Eric Martz at the request of Raymond J. Deshaies of CalTech.
