Flagellar filament of bacteria
From Proteopedia
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The structure of a straight L form of the flagellar filament was reported in 2010<ref name="3a5x">Maki-Yonekura, S., K. Yonekura, & K. Namba, 'Conformational change of flagellin for polymorphic supercoiling of the flagellar filament', Nat. Struct. Mol. Biol. 17, 417–422 (2010). [http://dx.doi.org/10.1038/nsmb.1774 doi:10.1038/nsmb.1774]</ref>. The monomer, [[3a5x]], was obtained by fitting the R-form monomer [[1ucu]] into the electron cryomicroscopy density map. | The structure of a straight L form of the flagellar filament was reported in 2010<ref name="3a5x">Maki-Yonekura, S., K. Yonekura, & K. Namba, 'Conformational change of flagellin for polymorphic supercoiling of the flagellar filament', Nat. Struct. Mol. Biol. 17, 417–422 (2010). [http://dx.doi.org/10.1038/nsmb.1774 doi:10.1038/nsmb.1774]</ref>. The monomer, [[3a5x]], was obtained by fitting the R-form monomer [[1ucu]] into the electron cryomicroscopy density map. | ||
| - | <Structure size='450' frame='true' align='right' caption='' scene='Flagellar_filament_of_bacteria/Yale_morph_r_to_l/ | + | <Structure size='450' frame='true' align='right' caption='' scene='Flagellar_filament_of_bacteria/Yale_morph_r_to_l/2' /> |
Below are morphs between the R and L forms of the monomer ([[1ucu]] and [[3a5x]] respectively) and the filaments. Filament models were kindly provided by T. Fujii and K. Namba. | Below are morphs between the R and L forms of the monomer ([[1ucu]] and [[3a5x]] respectively) and the filaments. Filament models were kindly provided by T. Fujii and K. Namba. | ||
| - | *R to L Monomer Morph (all residues aligned) (<scene name='Flagellar_filament_of_bacteria/Yale_morph_r_to_l/ | + | *R to L Monomer Morph (all residues aligned) (<scene name='Flagellar_filament_of_bacteria/Yale_morph_r_to_l/2'>restore initial scene</scene>)<ref>Chemically possible morph generated by the [http://molmovdb.org Yale Morph Server (molmovdb.org)], [[1ucu]] to [[3a5x]].</ref> |
==Notes== | ==Notes== | ||
<references /> | <references /> | ||
Revision as of 13:13, 14 May 2011
R vs. L forms
Quoted from [1]:
"The bacterial flagellar filament is a helical propeller rotated by the flagellar motor for bacterial locomotion. The filament is a supercoiled assembly of a single protein, flagellin, and is formed by 11 protofilaments. For bacterial taxis, the reversal of motor rotation switches the supercoil between left- and right-handed, both of which arise from combinations of two distinct conformations and packing interactions of the L-type and R-type protofilaments."
The structure of a straight R form of the flagellar filament was reported in 2003[2], by fitting and extending a crystallographic model of the monomer (1io1)[3] into an electron cryomicroscopic density map with resolution approaching 4 Å. The resulting full-length R form monomer, 1ucu, included terminal alpha helices that were absent in the crystallographic model.
The structure of a straight L form of the flagellar filament was reported in 2010[1]. The monomer, 3a5x, was obtained by fitting the R-form monomer 1ucu into the electron cryomicroscopy density map.
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Below are morphs between the R and L forms of the monomer (1ucu and 3a5x respectively) and the filaments. Filament models were kindly provided by T. Fujii and K. Namba.
- R to L Monomer Morph (all residues aligned) ()[4]
Notes
- ↑ 1.0 1.1 Maki-Yonekura, S., K. Yonekura, & K. Namba, 'Conformational change of flagellin for polymorphic supercoiling of the flagellar filament', Nat. Struct. Mol. Biol. 17, 417–422 (2010). doi:10.1038/nsmb.1774
- ↑ Yonekura K, Maki-Yonekura S, Namba K. Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy. Nature. 2003 Aug 7;424(6949):643-50. PMID:12904785 doi:http://dx.doi.org/10.1038/nature01830
- ↑ Samatey FA, Imada K, Nagashima S, Vonderviszt F, Kumasaka T, Yamamoto M, Namba K. Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling. Nature. 2001 Mar 15;410(6826):331-7. PMID:11268201 doi:10.1038/35066504
- ↑ Chemically possible morph generated by the Yale Morph Server (molmovdb.org), 1ucu to 3a5x.
