This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
Flagellar filament of bacteria
From Proteopedia
Contents |
R vs. L forms
Quoted from Maki-Yonekura, Yonekura and Namba, 2010[1]:
"The bacterial flagellar filament is a helical propeller rotated by the flagellar motor for bacterial locomotion. The filament is a supercoiled assembly of a single protein, flagellin, and is formed by 11 protofilaments. For bacterial taxis, the reversal of motor rotation switches the supercoil between left- and right-handed, both of which arise from combinations of two distinct conformations and packing interactions of the L-type and R-type protofilaments."[1]
The structure of a straight R form of the flagellar filament was reported in 2003[2], by fitting and extending a crystallographic model of the monomer (1io1)[3] into an electron cryomicroscopic density map with resolution approaching 4 Å. The resulting full-length R form monomer, 1ucu, included terminal alpha helices that were absent in the crystallographic model.
The structure of a straight L form of the flagellar filament was reported in 2010[1]. The monomer, 3a5x, was obtained by fitting the R-form monomer 1ucu into the L form electron cryomicroscopy density map.
R to L Morphs
|
Below are morphs between the R and L forms of the monomer (1ucu and 3a5x respectively) and the filament. Filament models were kindly provided by T. Fujii and K. Namba.
- R to L Monomer Morph (all residues aligned) ()[4]
- (Filament morphs are in preparation. Eric Martz 07:10, 15 May 2011 (IDT))
Notes
- ↑ 1.0 1.1 1.2 Maki-Yonekura, S., K. Yonekura, & K. Namba, 'Conformational change of flagellin for polymorphic supercoiling of the flagellar filament', Nat. Struct. Mol. Biol. 17, 417–422 (2010). doi:10.1038/nsmb.1774
- ↑ Yonekura K, Maki-Yonekura S, Namba K. Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy. Nature. 2003 Aug 7;424(6949):643-50. PMID:12904785 doi:http://dx.doi.org/10.1038/nature01830
- ↑ Samatey FA, Imada K, Nagashima S, Vonderviszt F, Kumasaka T, Yamamoto M, Namba K. Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling. Nature. 2001 Mar 15;410(6826):331-7. PMID:11268201 doi:10.1038/35066504
- ↑ Chemically possible morph generated by the Yale Morph Server (molmovdb.org), Job 076723-32427, file Image:1ucu-3a5x-yale-morph.pdb.gz.
- ↑ Linear interpolation morph, file Image:1ucu 3a5x linmorph.pdb.gz. See Morphs.
