1bso
From Proteopedia
(New page: 200px<br /><applet load="1bso" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bso, resolution 2.23Å" /> '''12-BROMODODECANOIC A...) |
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| - | [[Image:1bso.gif|left|200px]]<br /><applet load="1bso" size=" | + | [[Image:1bso.gif|left|200px]]<br /><applet load="1bso" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1bso, resolution 2.23Å" /> | caption="1bso, resolution 2.23Å" /> | ||
'''12-BROMODODECANOIC ACID BINDS INSIDE THE CALYX OF BOVINE BETA-LACTOGLOBULIN'''<br /> | '''12-BROMODODECANOIC ACID BINDS INSIDE THE CALYX OF BOVINE BETA-LACTOGLOBULIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The X-ray structure of bovine beta-lactoglobulin with the ligand | + | The X-ray structure of bovine beta-lactoglobulin with the ligand 12-bromododecanoic acid as a model for fatty acids has been determined at a resolution of 2.23 A in the trigonal lattice Z form. The ligand binds inside the calyx, resolving a long-standing controversy as to where fatty-acid like ligands bind. The carboxylate head group lies at the surface of the molecule, and the lid to the calyx is open at the pH of crystallization (pH 7.3), consistent with the conformation observed in ligand-free bovine beta-lactoglobulin in lattice Z at pH 7.1 and pH 8.2. |
==About this Structure== | ==About this Structure== | ||
| - | 1BSO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with BRC as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1BSO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=BRC:'>BRC</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BSO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Baker, E | + | [[Category: Baker, E N.]] |
| - | [[Category: Creamer, L | + | [[Category: Creamer, L K.]] |
| - | [[Category: Jameson, G | + | [[Category: Jameson, G B.]] |
| - | [[Category: Qin, B | + | [[Category: Qin, B Y.]] |
[[Category: BRC]] | [[Category: BRC]] | ||
[[Category: beta-lactoglobulin]] | [[Category: beta-lactoglobulin]] | ||
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[[Category: x-ray crystal structure]] | [[Category: x-ray crystal structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:58:39 2008'' |
Revision as of 09:58, 21 February 2008
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12-BROMODODECANOIC ACID BINDS INSIDE THE CALYX OF BOVINE BETA-LACTOGLOBULIN
Overview
The X-ray structure of bovine beta-lactoglobulin with the ligand 12-bromododecanoic acid as a model for fatty acids has been determined at a resolution of 2.23 A in the trigonal lattice Z form. The ligand binds inside the calyx, resolving a long-standing controversy as to where fatty-acid like ligands bind. The carboxylate head group lies at the surface of the molecule, and the lid to the calyx is open at the pH of crystallization (pH 7.3), consistent with the conformation observed in ligand-free bovine beta-lactoglobulin in lattice Z at pH 7.1 and pH 8.2.
About this Structure
1BSO is a Single protein structure of sequence from Bos taurus with as ligand. Full crystallographic information is available from OCA.
Reference
12-Bromododecanoic acid binds inside the calyx of bovine beta-lactoglobulin., Qin BY, Creamer LK, Baker EN, Jameson GB, FEBS Lett. 1998 Nov 6;438(3):272-8. PMID:9827560
Page seeded by OCA on Thu Feb 21 11:58:39 2008
