1fzc
From Proteopedia
(New page: 200px<br /> <applet load="1fzc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fzc, resolution 2.3Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1fzc.gif|left|200px]]<br /> | + | [[Image:1fzc.gif|left|200px]]<br /><applet load="1fzc" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1fzc" size=" | + | |
caption="1fzc, resolution 2.3Å" /> | caption="1fzc, resolution 2.3Å" /> | ||
'''CRYSTAL STRUCTURE OF FRAGMENT DOUBLE-D FROM HUMAN FIBRIN WITH TWO DIFFERENT BOUND LIGANDS'''<br /> | '''CRYSTAL STRUCTURE OF FRAGMENT DOUBLE-D FROM HUMAN FIBRIN WITH TWO DIFFERENT BOUND LIGANDS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Factor XIII-cross-linked fragment D (double-D) from human fibrin was | + | Factor XIII-cross-linked fragment D (double-D) from human fibrin was crystallized in the presence of two different peptide ligands and the X-ray structure determined at 2.3 A. The peptide Gly-Pro-Arg-Pro-amide, which is an analogue of the knob exposed by the thrombin-catalyzed removal of fibrinopeptide A, was found to reside in the gamma-chain holes, and the peptide Gly-His-Arg-Pro-amide, which corresponds to the beta-chain knob, was found in the homologous beta-chain holes. The structure shows for the first time that the beta-chain knob does indeed bind to a homologous hole on the beta-chain. The gamma- and beta-chain holes are structurally very similar, and it is remarkable they are able to distinguish between these two peptides that differ by a single amino acid. Additionally, we have found that the beta-chain domain, like its gamma-chain counterpart, binds calcium. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1FZC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG, MAN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1FZC with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb83_1.html Fibrin]]. Full crystallographic information is available from [http:// | + | 1FZC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=MAN:'>MAN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1FZC with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb83_1.html Fibrin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FZC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Doolittle, R | + | [[Category: Doolittle, R F.]] |
| - | [[Category: Everse, S | + | [[Category: Everse, S J.]] |
[[Category: Riley, M.]] | [[Category: Riley, M.]] | ||
[[Category: Spraggon, G.]] | [[Category: Spraggon, G.]] | ||
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[[Category: plasma protein]] | [[Category: plasma protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:44:14 2008'' |
Revision as of 10:44, 21 February 2008
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CRYSTAL STRUCTURE OF FRAGMENT DOUBLE-D FROM HUMAN FIBRIN WITH TWO DIFFERENT BOUND LIGANDS
Contents |
Overview
Factor XIII-cross-linked fragment D (double-D) from human fibrin was crystallized in the presence of two different peptide ligands and the X-ray structure determined at 2.3 A. The peptide Gly-Pro-Arg-Pro-amide, which is an analogue of the knob exposed by the thrombin-catalyzed removal of fibrinopeptide A, was found to reside in the gamma-chain holes, and the peptide Gly-His-Arg-Pro-amide, which corresponds to the beta-chain knob, was found in the homologous beta-chain holes. The structure shows for the first time that the beta-chain knob does indeed bind to a homologous hole on the beta-chain. The gamma- and beta-chain holes are structurally very similar, and it is remarkable they are able to distinguish between these two peptides that differ by a single amino acid. Additionally, we have found that the beta-chain domain, like its gamma-chain counterpart, binds calcium.
Disease
Known diseases associated with this structure: Afibrinogenemia, congenital OMIM:[134820], Afibrinogenemia, congenital OMIM:[134830], Amyloidosis, hereditary renal OMIM:[134820], Dysfibrinogenemia, alpha type, causing bleeding diathesis OMIM:[134820], Dysfibrinogenemia, alpha type, causing recurrent thrombosis OMIM:[134820], Dysfibrinogenemia, beta type OMIM:[134830], Dysfibrinogenemia, gamma type OMIM:[134850], Hypofibrinogenemia, gamma type OMIM:[134850], Thrombophilia, dysfibrinogenemic OMIM:[134830], Thrombophilia, dysfibrinogenemic OMIM:[134850]
About this Structure
1FZC is a Protein complex structure of sequences from Homo sapiens with , and as ligands. The following page contains interesting information on the relation of 1FZC with [Fibrin]. Full crystallographic information is available from OCA.
Reference
Crystal structure of fragment double-D from human fibrin with two different bound ligands., Everse SJ, Spraggon G, Veerapandian L, Riley M, Doolittle RF, Biochemistry. 1998 Jun 16;37(24):8637-42. PMID:9628725
Page seeded by OCA on Thu Feb 21 12:44:14 2008
Categories: Fibrin | Homo sapiens | Protein complex | Doolittle, R F. | Everse, S J. | Riley, M. | Spraggon, G. | Veerapandian, L. | CA | MAN | NAG | Blood coagulation | Crosslinking | Plasma protein
