Enolase multiple sequence alignment

From Proteopedia

Revision as of 23:31, 30 September 2012

Multiple Sequence Alignment (MSA) for enolase, an enzyme in glycolysis.

Key: IDENTICAL(*) SIMILAR(.) different Consensus Percentage is set to 100%.

 1    ENO_DROME| Drosophila melanogaster  <b>G</b>vh<b>EA</b>L<b>E</b>l<b>RD</b>
nD<b>K</b>anYh<b>GK</b>S <b>V</b>lk<b>AV</b>ghV<b>N</b>d tLgpelIkan ldvvd<b>Q</b>asI<b>D</b>
91<br>
 2              ENOA_HUMAN|
Homo sapiens  <b>G</b>iy<b>EA</b>L<b>E</b>l<b>RD</b> nD<b>K</b>trYm<b>GK</b>G
<b>V</b>sk<b>AV</b>ehI<b>N</b>k tIapalVskk lnvte<b>Q</b>ekI<b>D</b> 90<br>
 3    ENO_METJA| Methanococcus janaschii  <b>G</b>th<b>EA</b>L<b>E</b>l<b>RD</b>
kE<b>K</b>-rFg<b>GK</b>G <b>V</b>lm<b>AV</b>enV<b>N</b>s iIrpeiLg-- ydarm<b>Q</b>reI<b>D</b>
97<br>
 4           ENO_ECOLI|
Escherichia coli  <b>G</b>sr<b>EA</b>L<b>E</b>l<b>RD</b> gD<b>K</b>srFl<b>GK</b>G
<b>V</b>tk<b>AV</b>aaV<b>N</b>g pIaqalIg-- kdakd<b>Q</b>agI<b>D</b> 90<br>
 5          ENO_BACSU| Bacillus
subtilis  <b>G</b>ey<b>EA</b>V<b>E</b>l<b>RD</b> gD<b>K</b>drYl<b>GK</b>G <b>V</b>lt<b>AV</b>nnV<b>N</b>e
iIapelLg-- fdvte<b>Q</b>naI<b>D</b> 91<br>
 6  4ENL Yeast| Saccharomyces cerevisiae  <b>G</b>vh<b>EA</b>L<b>E</b>m<b>RD</b>
gD<b>K</b>skWm<b>GK</b>G <b>V</b>lh<b>AV</b>knV<b>N</b>d vIapafVkan idvsd<b>Q</b>kaV<b>D</b>
90<o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Courier New";
mso-bidi-font-family:"Courier New"'><span style="mso-spacerun:
yes">                                         
</span>*<span style="mso-spacerun: yes">  </span>**.* **<span
style="mso-spacerun: yes">  </span>.*<span style="mso-spacerun:
yes">  </span>. **. *<span style="mso-spacerun: yes">  </span>**<span
style="mso-spacerun: yes">  </span>.*<span style="mso-spacerun:
yes">   </span>.<span style="mso-spacerun: yes">   </span><span
style="mso-spacerun: yes"> </span>.<span style="mso-spacerun:
yes">         </span>*<span
style="mso-spacerun: yes">  </span>.*<br>
<br>
 1    ENO_DROME| Drosophila melanogaster  nfm<b>I</b>kl<b>DGT</b>e
<b>NK</b>SKf<b>GANAI</b> <b>L</b>G<b>VS</b>l<b>A</b>vaK<b>A</b> G<b>A</b>akkgv<b>PLY</b>
khIAdlagn- 140<br>
 2              ENOA_HUMAN|
Homo sapiens  klm<b>I</b>em<b>DGT</b>e <b>NK</b>SKf<b>GANAI</b> <b>L</b>G<b>VS</b>l<b>A</b>vcK<b>A</b>
G<b>A</b>vekgv<b>PLY</b> rhIAdlagn- 139<br>
 3    ENO_METJA| Methanococcus janaschii  tim<b>I</b>el<b>DGT</b>p
<b>NK</b>SRl<b>GANAI</b> <b>L</b>A<b>VS</b>l<b>A</b>vaK<b>A</b> A<b>A</b>ataki<b>PLY</b>
kyLGgfn--- 144<br>
 4           ENO_ECOLI|
Escherichia coli  kim<b>I</b>dl<b>DGT</b>e <b>NK</b>SKf<b>GANAI</b> <b>L</b>A<b>VS</b>l<b>A</b>naK<b>A</b>
A<b>A</b>aakgm<b>PLY</b> ehIAelngt- 139<br>
 5          ENO_BACSU|
Bacillus subtilis  qll<b>I</b>el<b>DGT</b>e <b>NK</b>GKl<b>GANAI</b> <b>L</b>G<b>VS</b>m<b>A</b>caR<b>A</b>
A<b>A</b>dflqi<b>PLY</b> qyLGgfn--- 138<br>
 6  4ENL Yeast| Saccharomyces cerevisiae  dfl<b>I</b>sl<b>DGT</b>a
<b>NK</b>SKl<b>GANAI</b> <b>L</b>G<b>VS</b>l<b>A</b>asR<b>A</b> A<b>A</b>aeknv<b>PLY</b>
khLAdlsksk 140<o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Courier New";
mso-bidi-font-family:"Courier New"'><span style="mso-spacerun:
yes">                                            
</span>*<span style="mso-spacerun: yes">  </span>***<span
style="mso-spacerun: yes">  </span>**.. ***** *.** *<span
style="mso-spacerun: yes">  </span>.* .*<span style="mso-spacerun:
yes">     </span>***<span style="mso-spacerun:
yes">    </span>.<br>
<br>
 1    ENO_DROME| Drosophila melanogaster  -keiil<b>PVP</b>a
f<b>N</b>VI<b>NGG</b>s<b>HA</b> gnkLam<b>QEFM</b> <b>I</b>l<b>P</b>t<b>GA</b>tsft
<b>EA</b>mKm<b>G</b>SeVY 189<br>
 2              ENOA_HUMAN|
Homo sapiens  -sevil<b>PVP</b>a f<b>N</b>VI<b>NGG</b>s<b>HA</b> gnkLam<b>QEFM</b>
<b>I</b>l<b>P</b>v<b>GA</b>anfr <b>EA</b>mRi<b>G</b>AeVY 188<br>
 3    ENO_METJA| Methanococcus janaschii  --syvm<b>PVP</b>m
m<b>N</b>VI<b>NGG</b>k<b>HA</b> gndLdl<b>QEFM</b> <b>I</b>m<b>P</b>v<b>GA</b>tsis
<b>EA</b>vRm<b>G</b>SeVY 192<br>
 4           ENO_ECOLI|
Escherichia coli  pgkysm<b>PVP</b>m m<b>N</b>II<b>NGG</b>e<b>HA</b> dnnVdi<b>QEFM</b>
<b>I</b>q<b>P</b>v<b>GA</b>ktvk <b>EA</b>iRm<b>G</b>SeVF 189<br>
 5          ENO_BACSU|
Bacillus subtilis  --sktl<b>PVP</b>m m<b>N</b>IV<b>NGG</b>e<b>HA</b>
dnnVdi<b>QEFM</b> <b>I</b>m<b>P</b>v<b>GA</b>pnfr <b>EA</b>lRm<b>G</b>AqIF 186<br>
 6  4ENL Yeast| Saccharomyces cerevisiae  tspyvl<b>PVP</b>f
l<b>N</b>VL<b>NGG</b>s<b>HA</b> ggaLal<b>QEFM</b> <b>I</b>a<b>P</b>t<b>GA</b>ktfa
<b>EA</b>lRi<b>G</b>SeVY 190<o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Courier New";
mso-bidi-font-family:"Courier New"'><span style="mso-spacerun:
yes">                                               
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style="mso-spacerun: yes">    </span>.<span style="mso-spacerun:
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<span style='font-size:10.0pt;font-family:"Courier New";mso-fareast-font-family:
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</span>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Courier New";
mso-bidi-font-family:"Courier New"'> 1    ENO_DROME|
Drosophila melanogaster  <b>H</b>h<b>L</b>knVikaK fgldata<b>VGD</b> <b>EGG</b>f<b>AP</b>nIqs
nk<b>E</b>A<b>L</b>nlIsd AIak<b>AG</b>yt-- 237<br>
 2              ENOA_HUMAN|
Homo sapiens  <b>H</b>n<b>L</b>knVikeK ygkdatn<b>VGD</b> <b>EGG</b>f<b>AP</b>nIle
nk<b>E</b>G<b>L</b>elLkt AIgk<b>AG</b>yt-- 236<br>
 3    ENO_METJA| Methanococcus janaschii  <b>H</b>v<b>L</b>knVileK
ygknavn<b>VGD</b> <b>EGG</b>f<b>AP</b>pLkt sr<b>E</b>A<b>L</b>dlLte SVkk<b>AG</b>ye--
240<br>
 4           ENO_ECOLI|
Escherichia coli  <b>H</b>h<b>L</b>akVlkaK g--mnta<b>VGD</b> <b>EGG</b>y<b>AP</b>nLgs
na<b>E</b>A<b>L</b>avIae AVka<b>AG</b>yelg 237<br>
 5          ENO_BACSU|
Bacillus subtilis  <b>H</b>s<b>L</b>ksVlsaK g--lnta<b>VGD</b> <b>EGG</b>f<b>AP</b>nLgs
ne<b>E</b>A<b>L</b>qtIve AIek<b>AG</b>fkpg 234<br>
 6  4ENL Yeast| Saccharomyces cerevisiae  <b>H</b>n<b>L</b>ksLtkkR
ygasagn<b>VGD</b> <b>EGG</b>v<b>AP</b>nIqt ae<b>E</b>A<b>L</b>dlIvd AIka<b>AG</b>hd—238<o:p></o:p></span></p>

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style='font-size:10.0pt;font-family:"Courier New";mso-bidi-font-family:"Courier New"'><span
style="mso-spacerun:
yes">                      
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yes">                   </span>*
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<span style="mso-spacerun:
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</span>~~~~~~~~~~~~~~~~~~~~~<br>
 1    ENO_DROME| Drosophila melanogaster  gkIeiGm<b>D</b>v<b>A</b>
A<b>SEF</b>Yk--d<b>G</b> q<b>Y</b>dLdfknek sdksqwlpad klanlykefi 285<br>
 2              ENOA_HUMAN|
Homo sapiens  dkVviGm<b>D</b>v<b>A</b> A<b>SEF</b>Fr--s<b>G</b> k<b>Y</b>dLdfksp-
ddpsryispd qladlyksfi 283<br>
 3    ENO_METJA| Methanococcus janaschii  deVvfAl<b>D</b>a<b>A</b>
A<b>SEF</b>Yk--d<b>G</b> y<b>Y</b>yVegkk-- ------ltre elldyykalv 280<br>
 4           ENO_ECOLI|
Escherichia coli  kdItlAm<b>D</b>c<b>A</b> A<b>SEF</b>Yk--d<b>G</b> k<b>Y</b>vLagegn-
----kaftse efthfleelt 280<br>
 5          ENO_BACSU|
Bacillus subtilis  eeVklAm<b>D</b>a<b>A</b> S<b>SEF</b>Ynked<b>G</b> k<b>Y</b>hLsgeg--
----vvktsa emvdwyeelv 278<br>
 6  4ENL Yeast| Saccharomyces cerevisiae  gkVkiGl<b>D</b>c<b>A</b>
S<b>SEF</b>Fk--d<b>G</b> k<b>Y</b>dLdfknpn sdkskwltgp qladlyhslm 286<o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Courier New";
mso-bidi-font-family:"Courier New"'><span style="mso-spacerun:
yes">                          
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yes">                 </span>.<span
style="mso-spacerun: yes">  </span>. * * .***.<span style="mso-spacerun:
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.<span style="mso-spacerun:
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</span><br>
<span style="mso-spacerun:
yes">                                         
</span>~~~~~~~~~~~~~~~~~~~~~<br>
 1    ENO_DROME| Drosophila melanogaster  kdF<b>P</b>IV<b>SIED</b>
pfdqdhWeAw snltgctd-- I<b>Q</b>I<b>V</b>G<b>DDL</b>t<b>V</b> <b>TN</b>pkrIatAV
333<br>
 2              ENOA_HUMAN|
Homo sapiens  kdY<b>P</b>VV<b>SIED</b> pfdqddWgAw qkftasag-- I<b>Q</b>V<b>V</b>G<b>DDL</b>t<b>V</b>
<b>TN</b>pkrIakAV 331<br>
 3    ENO_METJA| Methanococcus janaschii  deY<b>P</b>IV<b>SIED</b>
pfheedFeGf amitkeld-- I<b>Q</b>I<b>V</b>G<b>DDL</b>f<b>V</b> <b>TN</b>verLrkGI
328<br>
 4           ENO_ECOLI|
Escherichia coli  kqY<b>P</b>IV<b>SIED</b> gldesdWdGf ayqtkvlgdk I<b>Q</b>L<b>V</b>G<b>DDL</b>f<b>V</b>
<b>TN</b>tkiLkeGI 330<br>
 5          ENO_BACSU|
Bacillus subtilis  skY<b>P</b>II<b>SIED</b> gldendWeGh kllterlgkk V<b>Q</b>L<b>V</b>G<b>DDL</b>f<b>V</b>
<b>TN</b>tkkLseGI 328<br>
 6  4ENL Yeast| Saccharomyces cerevisiae  krY<b>P</b>IV<b>SIED</b>
pfaeddWeAw shffktag-- I<b>Q</b>I<b>V</b>A<b>DDL</b>t<b>V</b> <b>TN</b>pkrIatAI
334

Summary of categories applied to a total of 436 residues in the PDB file sequence for a consensus level setting of 100%: 33% (142) IDENTICAL(*) 16% (68) SIMILAR(.) 52% (226) different Definitions of SIMILAR groups of amino acids: ILV Ile Leu Val hydrophobic, aliphatic / medium-sized side-chain FWY Phe Trp Tyr hydrophobic, aromatic / large side-chain (Tyr polar) KRH Lys Arg His + charge DE Asp Glu - charge GAS Gly Ala Ser small P Pro helix breaking C Cys disulfide-forming TNQM Thr Asn Gln Met polar (except Met)