1x18
From Proteopedia
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| - | [[Image:1x18.png|left|200px]] | ||
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{{STRUCTURE_1x18| PDB=1x18 | SCENE= }} | {{STRUCTURE_1x18| PDB=1x18 | SCENE= }} | ||
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===Contact sites of ERA GTPase on the THERMUS THERMOPHILUS 30S SUBUNIT=== | ===Contact sites of ERA GTPase on the THERMUS THERMOPHILUS 30S SUBUNIT=== | ||
| + | {{ABSTRACT_PUBMED_15866174}} | ||
| - | + | ==Function== | |
| + | [[http://www.uniprot.org/uniprot/RS7_THET8 RS7_THET8]] One of the primary rRNA binding proteins, it binds directly to 3'-end of the 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center. Binds mRNA and the E site tRNA blocking its exit path in the ribosome. This blockage implies that this section of the ribosome must be able to move to release the deacetylated tRNA.[HAMAP-Rule:MF_00480_B] [[http://www.uniprot.org/uniprot/RS11_THET8 RS11_THET8]] Located on the upper part of the platform of the 30S subunit, where it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome, where it interacts both with the Shine-Dalgarno helix and mRNA.[HAMAP-Rule:MF_01310] [[http://www.uniprot.org/uniprot/RS18_THETH RS18_THETH]] Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit (By similarity). [[http://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8]] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B] | ||
==About this Structure== | ==About this Structure== | ||
| - | [[1x18]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X18 OCA]. | + | [[1x18]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/"flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X18 OCA]. |
==See Also== | ==See Also== | ||
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==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID:015866174</ref>< | + | <ref group="xtra">PMID:015866174</ref><references group="xtra"/><references/> |
| + | [[Category: Flavobacterium thermophilum yoshida and oshima 1971]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Agrawal, R K.]] | [[Category: Agrawal, R K.]] | ||
Revision as of 08:10, 18 December 2013
Contents |
Contact sites of ERA GTPase on the THERMUS THERMOPHILUS 30S SUBUNIT
Template:ABSTRACT PUBMED 15866174
Function
[RS7_THET8] One of the primary rRNA binding proteins, it binds directly to 3'-end of the 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center. Binds mRNA and the E site tRNA blocking its exit path in the ribosome. This blockage implies that this section of the ribosome must be able to move to release the deacetylated tRNA.[HAMAP-Rule:MF_00480_B] [RS11_THET8] Located on the upper part of the platform of the 30S subunit, where it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome, where it interacts both with the Shine-Dalgarno helix and mRNA.[HAMAP-Rule:MF_01310] [RS18_THETH] Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit (By similarity). [RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
About this Structure
1x18 is a 9 chain structure with sequence from "flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971 and Thermus thermophilus. Full crystallographic information is available from OCA.
See Also
- GTP-binding protein
- Ribosomal protein S11
- Ribosomal protein S18
- Ribosomal protein S2
- Ribosomal protein S7
Reference
- Sharma MR, Barat C, Wilson DN, Booth TM, Kawazoe M, Hori-Takemoto C, Shirouzu M, Yokoyama S, Fucini P, Agrawal RK. Interaction of Era with the 30S ribosomal subunit implications for 30S subunit assembly. Mol Cell. 2005 Apr 29;18(3):319-29. PMID:15866174 doi:10.1016/j.molcel.2005.03.028
