A Brief Description:
DdrB is a single-stranded DNA binding protein that is found in bacteria from the genus Deinococcus. DdrB is thought to mediate the repair of genomic DNA following extensive genomic fragmentation induced by radiation and other agents. In this role, DdrB’s function has been compared to that of a unique stress-inducible equivalent of a single-stranded DNA binding protein. However, its function is not limited to DNA binding, as the protein has also been implicated in suppressing Rec J exonuclease activity and promoting the annealing of complementary nucleotides.
General Description
DdrB is a 20kDa protein that is found in the bacterial genus Deinococcus. While it is not limited to Deinococcus radiodurans, its functional relevance has been clearly noted in this species. Deinococcus radiodurans is known for being a resilient bacteria that can withstand 15,000 Gy of γ radiation. This effectively shatters the genome into hundreds of 20-30kB fragments. However, this bacteria is able to accurately repair its genome in a matter of hours and survive. This is an especially remarkable feat considering that the lethal dose of γ radiation in humans is 2-10 Gy. As a result, this finding has lead researchers to ask why Deinococcus radiodurans is able to survive such high levels of radiation? This ability is not due to any notable alterations in the bacteria’s DNA. In other words, the radiation still induces many double stranded breaks (which are a prime contributor to lethality in other organisms). Instead, the resistance to radiation-induced death has been attributed to two components 1.) reactive oxygen species scavengers and 2.) a unique repair pathway.
The repair pathway in Deinococcus radiodurans is thought to be a two step process. In the first part of repair, 5’ exonucleases generate long 3’ ssDNA extensions in the DNA fragments. The second part of the repair process involves the extensions being put together through RecA mediated homologous recombination or through single-strand annealing. These processes are also accompanied by an induction in the expression of a variety of proteins. DdrB is one of the proteins that becomes highly upregulated following extensive DNA damage. This has been confirmed through evaluation of mRNA transcripts and mass spectrometry proteomic analysis. These studies revealed that DdrB is one of the top five upregulated genes following radiation exposure. To be specific, DdrB expression increases by over 40 fold following exposure to 3,000 Gy of γ radiation. This is in sharp contrast to single stranded DNA binding proteins, which only have a minor increase in expression. As a result, DdrB is thought to act as stress inducible equivalent of a single stranded DNA binding protein protecting and stabilizing any ssDNA that is present in the repair process. To further support the idea that DdrB has an essential role in the repair process of Deinococcus radiodurans, bacteria with no expression of DdrB experienced a 100 fold decrease in viability compared to the wild-type following exposure to 10,000 Gy of γ radiation.
Subsequent studies have revealed that this protein is unique to the Deinococcus genus and varies in primary sequence length and composition depending upon the species. However, the protein structure and function remains similar. Electromobility shift assays have been used to further define DdrB function in terms of binding to nucleic acid. These experiments have shown that DdrB preferentially binds to ssDNA with low uM affinity. These studies also showed that DdrB has slight affinity for RNA and does not bind to double-stranded DNA. Even more recently, studies have shown that the function of DdrB is not limited to ssDNA binding. It has also been shown to promote the annealing of complementary oligonucleotides and surprisingly has been shown to suppress RecJ exonuclease activity, further implicating DdrB in the repair of fragmented genomic DNA.
Structure Description
DdrB Monomers
Crystal Structures have revealed that DdrB is a mulitmeric protein composed of . The units contain 1 alpha helix, 4 3/10 helices, 8 beta strands, 4 beta turns, 5 beta bends, and regions of undefined structure.
The N-terminal Domain
The N-terminal domain contains a . The is amphipathic, in which the hydrophobic regions pack toward features in the DdrB core.
The DdrB Core
This is followed by 6 , , which contain a solvent exposed face and another face that against the N-terminal motif. The beta sheets are anti-parallel and do not form an OB fold as determined by multiple servers iCOPS, DALI, 3D-BLAST, and MATRAS. At the time of this finding (2010), the lack of an OB fold was surprising, since all ssDNA binding proteins were thought to bind to DNA through an OB fold. The OB fold is two three-stranded anti-parallel β sheets that form a five stranded β barrel. The OB folds adopt greek key motifs. The differences between the DdrB beta strands and those in the OB fold include the topology of the β strands. DdrB strands form an up and down topology and not a Greek key. Furthermore, monomeric DdrB β strands do not form a beta barrel. Additionally, DdrB has different connectivity, no conserved glycine, and no β bulge.
Positively Charged amino acids reside in the solvent exposed beta strands, which may potentially enable the binding of ssDNA.
that link beta sheet 6 to sheet 7 and beta sheet 7 to sheet 8 contain flexible regions with poor order as determined by limited to no density in the crystal structure. This finding suggests that these loops are intrinsically disordered.
The C-terminal Domain
The C-terminal domain also contains regions with predicted intrinsic disorder. The PSIpred server predicts that the last 35 residues of Deinococcus geothermalis are disordered. This prediction is supported by the solved crystal structure as the last 51 residues could not be determined. While, the structure of the C-terminal end is not known, it may still be of interest. A BLAST search revealed an 83 amino acid protein in Deinococcus geothermalis with 72% similarity and 62% identity to the disordered region of the C-terminus. However, the function of this protein remains unknown. One hypothesis is that this region may mediate protein-protein interactions. Single stranded binding proteins also have disordered C-termini and contain negatively charged residues that mediate protein-protein interactions. As DdrB contains several conserved negatively charged residues it is thought that the C-terminus of this protein could also mediate protein-protein interactions. However, this hypothesis may be debated as the C-terminus was not needed for radioresistance in Deinococcus radiodurans.
DdrB Pentamer
The monomeric units of DdrB collectively form a pentameric ring with a 10 A pore in the center of this structure. Other DNA binding proteins can thread DNA through a central pore, however this seems highly unlikely in the case of DdrB. The pore size appears too small (would need to be 14-40A) and has a net negative charge making it highly unfavorable for DNA interactions.
The pentamer also contains a positive residue track on one side of the pentamer. These residues facilitate ssDNA binding and DdrB functionality.
DdrB is a multimeric protein composed of . Each of the units share a at the N-terminus. This is followed by a series of , in which some are solvent exposed and others pack against the N terminal motif.
Flexible regions with poor order include that link beta sheet 6 to sheet 7 and beta sheet 7 to 8. These sheets have such limited order that the crystal structure could not be solved for some of the residues as their was little to no density. This lack of order suggests that these regions may be intrinsically disordered.
The beta sheets of the N-terminal beta-beta-alpha motif form a 10 stranded anti-parallel B-barrel. The barrel gives rise to a pore in the middle of the structure with approximately a 10A diameter. This structure is stabilized by interactions with the alpha helices?. Also prevents about 30% of solvent accessible area of the monomer from being exposed to the surrounding environment.
Structural features that relate to function
Related Proteins
No proteins are classified as directly related
However, different regions of the DdrB protein share common features with other proteins.
The C-terminal end of the DdrB protein appears to be related to a sequence within the Deinococcus geothermalis. This sequence encodes a 83 amino acid protein with unknown function. There is a 72% similarity and a 63% identity.
The C-terminal region of single stranded DNA binding proteins also tend to be disordered (about 60 residues of the C-terminus). Negatively charged residues in this disordered region has been shown to enable protein-protein interactions. Interestingly, DdrB has some negatively charged residues in this disordered region as well. This similarity may suggest that DdrB mediates DNA repair through similar protein-protein interactions.
