Phospholipase A2

From Proteopedia

Revision as of 06:31, 20 August 2014

spinqualitylabelsall models Phospholipase A2 complex with ethanol, phosphate and Ca+2 ion (green) (PDB code 1yxh) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Phospholipase A2 (PLA2) is an enzyme which releases fatty acids from glycerol. It is found in mammals and in snake venoms. PLA2 releases arachidonic acid from membranes causing inflammation and pain. PLA2 from the snake Bothrops jararacussu is called bothropstoxin (BTX) and from Bothrops pirajai – piratoxin (PTX). The viperotoxin (VTX) is a heterodimer of very homologous PLA2 called RV-4/RV-7. The intracellular PLA2 – cystolic PLA2 (cPLA2) – are larger than the secreted PLA2 and contains the targeting C2 domain. The pro-phosphlipase (PPLA2) is a pancreatic PLA2 whose 7-mer N-terminal peptide is being cleaved off to produce the active PLA2. The PLA2 contains many isozymes which are ordered by groups and named accordingly, ie., group I is PLA2G1. The images at the left and at the right correspond to one representative PLA2, i.e. the crystal structure of Phospholipase A2 from Naja naja sagittifera (1yxh). For details on Lys49 snake-venom PLA2 see Anum-II. For PLA2 complex see Diclofenac binding to Phospholipase A2. Crystal structure of porcine pancreatic phospholipase A2 in complex with 2-methoxycyclohexa-2-5-diene-1,4-dione [1] possesses anti-inflammatory activity. The binding of curcumin with PLA2 was studied using X-ray crystallography. Since the electron density found in the active site did not match with curcumin, (the photo-degraded product of curcumin) in the unexplained electron density. To understand the , molecular docking studies was carried out. with respect to the ligand position and identified that of PLA2 with a binding energy -16.81 Kcal/mol. The binding mode is in such a manner that it can prevent the entry of substrate to the hydrophobic active site. These studies indicate that curcumin can be act as an inhibitor to PLA2.

3D Structures of Phospholipase A2

Updated on 20-August-2014

PLA2

1une, 1mkt, 1irb, 1bp2, 1bpq, 1g4i, 2bp2, 2bpp - bPLA2 – bovine
2zp3, 2zp4, 2zp5, 1c74, 1kvw, 1kvx, 1kvy, 1ceh, 1mks, 1mku, 1vkq, 1vl9, 2bax, 2bch, 2bd1, 1o3w, 1gh4 – bPLA2 (mutant) – bovine
1bvm – bPLA2 - NMR
1qll, 2q2j – PLA2 – Bothrops pirajai
2qog – PLA2 – Crotalus durissus
2ph4 – PLA2 – Zhaoermia mangshanensis
2h4c, 1q6v – IvPLA2 – Indian viper
1vpi – PLA2 – sand viper
2osn, 1fe5, 1dpy – kPLA2 – krait
1g0z, 1g2x – kPLA2 (mutant)
2wg7, 2wg8 – rPLA2 – rice
1p2p, 4p2p – pPLA2 - pig
1y6p, 2phi, 3p2p – pPLA2 (mutant) – pig
1sfv, 1sfw, 1pir, 1pis – pPLA2 - NMR
1yxh – NsPLA2 – Naja sagittifera
1oz6, 2qhe – PLA2 – Echis carinatus
1it4, 1it5 – PLA2 – Stretomyces violaceoruber – NMR
1mg6 – PLA2 – Agkistrodon acutus
1s8g, 1s8h, 1s8i – PLA2 – copperhead
1ijl – PLA2 – Deinagkistrodon acutus
1mc2, 4hg9 – PLA2 – Chinese moccasin
1c74, 1kvw, 1kvx, 1kvy, 1ceh – bPLA2 (mutant) – bovine
1b4w, 1jia, 1c1j – AhPLA2 - Agkistrodon halys
1a2a, 1m8r, 1m8s, 1psj – PLA2 – Gloydius halys
1a3d, 1a3f, 1ln8, 1mh2, 1mh8, 1ows, 1poa, 1psh, 1s6b, 1sz8, 1xxw, 1y75, 1yxl, 1yxh – PLA2 – Naja naja
1mh7 - PLA2 – Naja sagittifera
2osh – PLA2 – Chinese cobra
1ae7, 2not, 4e4c – PLA2 – Notechis scutatus
1aok, 1jlt, 1q5t, 1rgb, 3dih, 3g8g, 3g8h – PLA2 – Vipera ammodytes
2i0u – PLA2 – Vipera nikolskii
1ayp, 1bbc, 1pod – hPLA2 – human
1rlw – hPLA2 CALB domain
1faz, 1kp4 – PLA2 – Streptomyces violaceruber
1god – PLA2 – Cerrophidion godmani
1gp7, 1m8t – PLA2 – Ophiophagus hanna
1ozy, 1p7o, 1pwo – PLA2 – Micropechis ikahena
1pp2 – PLA2 – rattlesnake
1ppa, 1vap – PLA2 – cottonmouth
1u4j – PLA2 – Bungarus caeruleus
2aoz – PLA2 – Atropoides nummifer
3v9m – PLA2 – king brown snake

PLA2 group I

3elo – hPLA2G1B
3q4y - PLA2G1 - Andaman cobra
3fvi, 3fvj – pPLA2G1B+octyl sulfate
3qlm - pPLA2G1B + hexadecanoic acid
3l30, 3l69, 3tt5, 4dbk - pPLA2G1B+berberine derivative
4g5i – pPLA2 + DBP
4o1y – pPLA2 + naphthalene acetic acid
3osh – NnPLA2G1+atropine

PLA2 group II

1j1a, 3u8b – hPLA2G2A
1n28, 1n29 – hPLA2G2A (mutant)
1cl5, 1fb2, 1vip, 2pvt, 2pyc – DrPLA2 – Daboia russellii
2qhw – DrPLA2 + gramine derivative
2que – DrPLA2 + ajamaline + anisic acid
1kqu, 1kvo – hPLA2G2A+substrate analog
3u8d, 3u8h, 3u8i, 4hmb - hPLA2G2A + inhibitor
3g8f – DrPLA2G2+polypeptide
3mlm – PLA2G2 + myristic acid – Neuwiedi lancehead
4fga, 4gfy, 4gld – DrPLA2 + polypeptide

PLA2 group V

4h0q – PLA2 – chinese green tree viper

Human PLA2 group X

1le6, 1le7 – hPLA2G10

Human PLA2 group XVI

2kyt, 4dot, 4fa0 – hPLA2G16 N-terminal

PLA2 group XIII

4aup – PLA13 – whitish truffle

PLA2+inhibitor

3o4m, 3hsw – PLA2+inhibitor
2azy, 2azz, 2b00, 2b01, 2b03, 2b04 – pPLA2+cholate derivative
1y6o, 1fx9, 1fxf – pPLA2+MJ33
1l8s – pPLA2+LPC-ether
5p2p – pPLA2+substrate analog
3nju – PLA2 group I+4-methoxy-benzoic acid – Andaman cobra
2wq5 – PLA2+minocyclin – Indian cobra
1oxl – IcPLA2+indole
3h1x, 3fo7 – IvPLA2+indomethacin
3fg5 – IvPLA2+pentapeptide+ajmaline
3cbi – IvPLA2+anisic acid+ajmaline
2qu9 – IvPLA2+eugenol
2otf – IvPLA2+atenolol
1zwp - IvPLA2+nimesulide
1th6 - IvPLA2+atropine
1td7 - IvPLA2+niflumic acid
1tgm - IvPLA2+aspirin
2wg9 – rPLA2+octanoic acid
1oxr – NsPLA2+aspirin
1bk9 – AhPLA2+PBPB
1fdk – bPLA2+MJ33
1mkv – bPLA2+transition state analog
1o2e – bPLA2 (mutant)+anisic acid
2b96 - bPLA2+benzoic acid derivative
3bp2 – bPLA2+pyruvic acid
1pob -NnPLA2+transition state analog
1poc - PLA2+transition state analog – Honey bee
1db4, 1db5, 1dcy – hPLA2+indole
1poe – hPLA2+phosphonyl inhibitor
1fv0 – DrPLA2+aristolochic acid
1sv3 - DrPLA2+benzoic acid derivative
1tp2, 2pws, 2q1p – DrPLA2+fatty acid
1sv9, 1sxk, 1zyx, 2b17, 2qvd – DrPLA2+anti-inflammatory agent
1kpm – DrPLA2+vitamin E
1oyf – DrPLA2+venom-6 methyl-heptanol
1q7a – DrPLA2+oxyphenabutazone
1y38 – DrPLA2+glycerophosphate
1zr8 – DrPLA2+ajmaline
2arm – DrPLA2+atropine
2dpz – DrPLA2 +hydroxyphenyl acetamide
2oli, 2oth, 2oyf – DrPLA2+indole derivative
2oub – DrPLA2+atenolol
2pmj – DrPLA2+benzopyrone
2zbh – DrPLA2+bavachalcone
4eix - DrPLA2+indomethacin + nimesulide
1po8, 1tc8 – kPLA2+fatty acid
1xxs – PLA2+fatty acid – Bothrops moojeni
2qhd – EcPLA2+fatty acid
3bjw – EcPLA2+suramin
1y4l – PLA2+suramin – Bothrops asper

PLA2+polypeptide

2o1n, 2d02, 2fnx, 1zm6, 1tdv, 1tg4, 1t37 - IvPLA2+polypeptide
1jq8, 1jq9 - DsPLA2+polypeptide
1mf4, 2rd4, 3gci, 3jq5, 3jql, 3jti - NnPLA2+polypeptide
1skg, 1sqz, 1tg1, 1tj9, 1tjk, 1tk4, 2do2, 2g58, 2gns, 2pb8 - DrPLA2+polypeptide

Bothrops toxins

3i3h, 3i3i, 3hzd, 2oqd, 1zlb, 1umv, 1u73, 1zl7 – BTX
3jr8 – BTXD+Ca
3hzw, 3i03 – BTX+BPB
3iq3, 2h8i – BTX + PEG
1z76 – BTX+bromophenacyl
1gmz, 1qll, 2q2j, 2ok9 - PTX
3cyl – PTX+α-tocopherol

Viperotoxin

1oqs – DrRV4/RV7

cPLA2

1bci – h-cPLA2 C2 domain
1cjy - h-cPLA2

Pro-PLA2

1hn4 – pPPLA2+MJ33
4bp2 – bPPLA2

References

1. ↑ Crystal structure of porcine pancreatic phospholipase a2 in complex with 2-methoxycyclohexa-2-5-diene-1,4-dione. Dileep KV, Tintu I, Mandal PK, Karthe P, Haridas M, Sadasivan C. Frontiers In Life Sci. (2012) doi:http://dx.doi.org/10.1080/21553769.2012.689262