Heat Shock Proteins

From Proteopedia

Revision as of 09:59, 27 August 2014

Template:STRUCTURE 3los

Heat Shock Proteins (Hsp). The Hsp are proteins which are expressed when cells are exposed to stresses like heat. Hsp60, Hsp70, Hsp90 etc. are named according to their molecular weights. Their function is to provide protection from stress. The image at the right corresponds to one representative Hsp, i.e. the crystal structure of Hsp60 from Methanococcus maripaludis (3los).

3D Structures of Heat Shock Proteins

Updated on 27-August-2014

Small Hsp

1shs, 4eld – Hsp – Methanococcus jannaschii
1gme – Hsp 17kD – wheat
2bol – Hsp – Taenia saginata
2h50, 2h53 – yHsp26 – yeast
2l9q, 2ljl, 4axp – yHsp12 - NMR
2wj5 – rHsp20 crystallin domain – rat
2wj7, 3l1g – hHspB crystallin domain – human
3q9p, 3q9q – hHsp28 β1
3bbu – EcHsp15 - Escherichia coli – Cryo-EM
1ons – EcHsp31
3gla, 3gt6, 3guf – Hsp – Xanthomonas axonopodis
3vqk – StHsp14 – Sulfolobus tokodaii
3vql – StHsp14 (mutant)
3w1z – Hsp16 – Schizosacchromyces pombe

Hsp33

1hw7, 3m7m – EcHsp33 N-terminal
1i7f – EcHsp33 chaperone domain (mutant)
1xjh - EcHsp33 C-terminal - NMR
1vq0 – Hsp33 – Thermotoga maritima
1vzy – Hsp33 – Bacillus subtilis

HslV, HslU, HslJ

1e94, 1yyf – EcHslV+HslU
2kts – EcHslJ - NMR
1g41 – HiHslU – Haemophilus influenza
1im2 – HiHslU NBD

Hsp40

1c3g – yHsp40 C-terminal peptide binding domain
2qld – hHsp40
1hdj – hHsp40 J domain - NMR

Hsp60 - the large subunit of the chaperonin molecule GroEL

3los – MmHsp60 - GroEL – Methanococcus maripaludis – closed state
3iyf - MmHsp60 - GroEL – open state
3e76 – EcHsp60 – GroEL+Tl ion – Escherichia coli
2nwc, 1oel, 1grl, 1la1 - EcHsp60 – GroEL
2eu1 - EcHsp60 (mutant) – GroEL
2cgt - EcHsp60 – GroEL+ADP+capsid assembly protein Gp31
2c7c, 2c7d - EcHsp60 – GroEL+GroES – cryo EM
1we3, 1wf4 - TtHsp60 – GroEL+GroES – Thermus thermophilus
1gr5 - EcHsp60 – GroEL – cryo EM
1gru - EcHsp60 – GroEL+ATP+GroES+ADP – cryo EM
1dkd - EcHsp60 – GroEL apical (top) domain (146 amino acids)+12-mer peptide
1dk7, 1jon - EcHsp60 – GroEL apical domain
1srv - TtHsp60 – GroEL apical domain
1ass, 1asx - Hsp60 – GroEL apical domain – Thermoplasma acidophilum
1kid - EcHsp60 (mutant) – GroEL apical domain

Hsp70 – 3 domains: N-terminus ATPase hydrolyzes ATP to ADP (NBD); hydrophobic amino acid binding domain (sub): C-terminus lid domain (lid).

3a8y – hHsp70 NBD+BAG domain regulator protein – human
3fzf - hHsp70 NBD+BAG domain regulator protein+ATP
3fzh, 3fzm, 3ldq, 3m3z - hHsp70 NBD+BAG domain regulator protein+ adenosine derivative
3fzk, 3fzl - hHsp70 NBD+BAG domain regulator protein+ purine derivative
3cqx - Hsp70 NBD+BAG domain regulator protein – mouse
3lof – hHsp70 lid
2lmg – hHsp70 C terminal - NMR
1xqs – hHsp70 sub + Hsp-binding protein core domain
2p32 - CeHsp70 lid
3l6q - CpHsp70 NBD – Cryptosporidium parvum
3kvg - CpHsp70 NBD+AMPPNP
3l4i - CpHsp70 NBD+ADP+P
3jxu, 3i33, 3gdq, 3fe1 - hHsp70 NBD+ADP+P
3iuc, 3atu, 3atv, 3ay9 - hHsp70 NBD+ADP
4j8f - hHsp70 NBD/HIP middle domain
2e88, 1s3x, 1hjo - hHsp70 NBD
2e8a - hHsp70 NBD+AMPPNP
2qw9 – bHsp70 NBD – bovine
2bup, 1bup, 1qqm, 1qqn - bHsp70 NBD (mutant)
2qwl, 2qwm, 1atr, 1ats, 1hpm, 1nga, 1ngb, 1ngc, 1ngd, 3hsc - bHsp70 NBD+ADP
1qqo – bHsp70 (mutant)
2qwn, 2qwo, 2qwp, 2qwq, 2qwr - bHsp70 NBD (mutant)+ auxilin protein
1ba0, 1ba1 – bHsp70 NBD (mutant) + ADP
1kax, 1kay, 1kaz - bHsp70 NBD (mutant) + ATP
1nge, 1ngf, 1ngg, 1ngh - bHsp70 NBD + ATP
1ngi, 1ngj - bHsp70 NBD + phosphoaminophosphonic acid
1hx1 - bHsp70 NBD + molecular chaperone regulator 1 BAG domain
2kho – EcHsp70 – Escherichia coli – NMR
1q5l - EcHsp70 sub domain+peptide - NMR
3gl1 - ScHsp70 NBD – Saccharomyces cerevisiae
3dpo – EcHsp70 sub+12-mer peptide
3dpp, 3dpq – EcHsp70 sub+20-mer peptide
3dob, 3dqg, 2op6 - CeHsp70 sub – Caenoharbditis elegans
4i2w – CeHsp70 + myosin chaperone
2d2e, 3d2f – ScHsp70 NBD+SSE1(Hsp110)
2v7z – rHsp70 NBD+sub – rat
7hsc, 1ckr - rHsp70 sub - NMR
3lca – yHsp70 Ssa1 C-terminal+yTOM71
2v7y – Hsp70 NBD+sub – Geobacillus kaustophilus

Hsp83

3u67 – LmHsp83 NTD + ADP – Leishmania major

Hsp90 – 4 domains: N-terminus (NTD); linker; middle (MD); C-terminus (CT)

Hsp90 NTD

3t0h - hHsp90-α NTD – human
3t0z, 3t2s - hHsp90-α NTD + ATP derivative
3t10 - hHsp90-α NTD + ACP
3t1k - hHsp90-α NTD + ANP
3k97, 3hek, 3mnq – hHsp90 NTD+pyrrolidin derivative – human
3k98, 3k99, 3qtf - hHsp90 NTD+indole derivative
3r91 - hHsp90 NTD (mutant) +indole derivative
3hhu, 4jql - hHsp90 NTD+ carbamic acid derivative
3ft8, 3ft5 - hHsp90 NTD+quinazoline derivative
2k5b - hHsp90 NTD+Cdc37 – NMR - docking
3eko, 3ekr - hHsp90 NTD+dihydroxyphenyl amide
3bm9, 3bmy - hHsp90 NTD+benzisoxazole
2of6, 3b24, 3b27, 3b28, 3r92 - hHsp90 NTD+triazine derivative
2qfo, 3nmq, 3r4m, 3r4n, 3r4o, 3r4p, 3b25, 3b26, 2jcc, 3rlp, 3rlq, 3rlr, 2wi1, 2wi2, 2wi3, 2wi4, 2wi5, 2wi6, 2wi7, 2jjc - hHsp90 NTD+pyrimidine derivative
1uy6, 1uy7, 1uy8, 1uy9, 1uyc, 1uyd, 1uye, 1uyf, 1uyg, 1uyh, 1uyi, 1uyk, 1uyl - hHsp90 NTD+purine derivative
2qg0, 2bz5 - hHsp90 NTD+sulfonamide derivative
2yi0, 2yi5, 2yi6, 2yi7 - hHsp90 NTD + thiadiazole derivative
4hy6 - hHsp90 NTD + indazole derivative
2qg2, 2cvi, 2cvj, 2uwd, 4b7p - hHsp90 NTD+oxazole derivative
3d0b, 3rkz - hHsp90 NTD+benzamide derivative
3inw, 3inx - hHsp90 NTD+pochoxime derivative
2fwy, 2fwz, 2h55, 1uym, 3o0l - hHsp90 NTD+PU inhibitor
2ccs, 2cct, 2ccu, 2cdd, 2byh, 2byi, 2bsm, 2bt0, 1yc3, 1yc1, 1yc4 - hHsp90 NTD+pyrazol derivative
2ye2, 2ye3, 2ye4, 2ye5, 2ye6, 2ye7, 2ye8, 2ye9, 2yea, 2yeb, 2yec, 2yed, 2yee, 2yef, 2yeg, 2yeh, 2yei, 2yej, 2yjw, 2yjx, 2yk2, 2yk9, 2ykb, 2ykc, 2yke, 2yki, 2ykj, 3ow6, 3owb, 3owd, 3qdd, 3tuh, 3vha, 3vhc, 3vhd, 4awo, 4awp, 4awq, 4eeh, 4eft, 4efu, 4egh, 4egi, 4egk, 4fcp, 4fcq, 4fcr- hHsp90 NTD + inhibitor
1byq - hHsp90 NTD+ATP-Mg
2xhr, 2xht, 2xhx, 2xjg, 2xjj, 2xjx, 2xk2, 2xdx, 2xdu, 2xds, 2xdl, 2xdk, 2xab, 2vcj, 2vci, 3hyy, 3hyz, 3hz1, 3hz5, 3mnr - hHsp90 NTD+ antigen
2brc, 2bre, 1am1 - yHsp90 NTD+pyrazol derivative
1amw - yHsp90 NTD+ADP
1ah6, 1ah8 - yHsp90 NTD
1osf, 1yer, 1yes, 1yet - hHsp90 NTD+geldamycin
3ied - PfHsp90 NTD+AMPPN – Plasmodium falciparum
3k60 - PfHsp90 NTD+ADP
2jki - Hsp90 NTD+SGT1 domain protein – barley
2h8m, 2hch – dHsp90 NTD – dog
2hg1 - dHsp90 NTD+ligand
2xcm – Hsp90 NTD+CGT1+RAR1 – Hordum vulgare
3h80 – LmHsp90 NTD
3q5j, 3q5k, 3q5l - LmHsp90 NTD + inhibitor
3o6o, 3omu, 3opd – TbHsp90 NTD+inhibitor – Trypanosoma brucei
4gqt – CeHsp90 NTD

Hsp90 MD

3pry – hHsp90 MD
2gq0 - EcHsp90 MD

Hsp90 CT

1sf8 – EcHsp90 CT
3hjc - LmHsp90 CT

Hsp90 NTD + MD

2o1w - dHsp90 NTD+MD

Hsp90 MD + CT

3q6m, 3q6n - hHsp90 MD + CT
2o1t – dHsp90 MD+CT

1bgq – yHsp90+radicicol
1a4h, 2weq – yHsp90+ geldamycin
1y4s, 1y4u, 2iop, 2ior - EcHsp90+ADP
2ioq - EcHsp90
2o1u – dHsp90+AMPPNP
2o1v - dHsp90+ADP
2qf6 - hHsp90 + antigen

Hsp82 is the yeast equivalent of Hsp90 in mammals

1ah6, 1ah8 – yHsp82 NTD1ah6, 1ah8 – yHsp82 NTD
2xd6 - yHsp82 NTD+macrolactone
2wep – yHsp82 NTD (mutant)+ADP
2akp - yHsp82 NTD (mutant)
1weq, 3c11 - yHsp82 NTD (mutant)+ geldamycin
2wer - yHsp82 NTD (mutant)+radicicol
1bgq - yHsp82 NTD +radicicol
2iws, 2iwu, 2iwx, 2cgf - yHsp82 NTD +radicicol analogues
2vw5, 2vwc - yHsp82 NTD +7-o-carbamoylpremacbecin
3c0e - yHsp82 NTD (mutant)
2fxs - yHsp82 NTD +radamide
1zw9 - yHsp82 NTD + adenine derivative
1zwh - yHsp82 NTD + radester amine
2brc, 2bre - yHsp82 NTD+pyrazol derivative
2cg9 – yHsp82+co-chaperone SBA1
2cge - yHsp82 MD+CT+co-chaperone SBA1
1hk7 - yHsp82 MD
1usu, 1usv - yHsp82 MD+co-chaperone AHA1
1us7 - yHsp82 NTD+co-chaperone cdc37
3fp2 – yHsp82 CT+TOM71

Hsp100 are named ClpA or ClpB

1r6b, 1ksf – EcClpA (mutant)
1khy – EcClpB NTD
1jbk – EcClpB NBD
1k6k - EcClpA NTD

Hsp110 in mammals is named Sse1, Sse2 in yeast

3d2e, 3d2f, 3c7n – ySse1+Hsp70
2qxl – ySse1
2llv – ySti1 domain 1 – NMR
2llw – ySti1 domain 2 – NMR
3upv – ySti1 TPR repeats 7-9 + ySse1 peptide
3uq3 - ySti1 TPR repeats 4-9 + peptide