Tobacco Etch Virus (TEV) Protease

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<applet load='1lvm' size='450' scene='User:Ashley_Steere/Sandbox_1/Tev_protease_monomer_1lvm/7'/, CAPTION='Dimer of monomeric TEV protease catalytic domain (grey and green) complex with polypeptide substrate (pink and yellow) and acetate, [[1lvm]]'>
The [http://en.wikipedia.org/wiki/Tobacco_etch_virus tobacco etch virus] (TEV) is a member of the ''Potyviridae'' family of positive-strand RNA viruses <ref>PMID:9129643</ref>. Unlike its name suggests, TEV can infect not only the tobacco plant, but a number of other plants including some flowers (baby's breath and zinnias) and fruits/vegetables (eggplant, peppers, and tomatoes). The 5' end of the RNA genome is covalently linked to a 49 kDa virus-encoded protein or VPg (viral protein genome-linked).The positive sense genome is translated by the host's ribosomes into a large polyprotein precursor that is cleaved soon after translation to form independent protein products <ref>PMID:2254747</ref>. The TEV nuclear inclusion a (NIa) protease (shown to the right co-crystallized with peptide product in <font color='purple'><b>purple</b></font>, with the catalytic residues highlighted as <span style="color:green;background-color:black;font-weight:bold;">green</span> ball and stick models) <ref name="Phan">PMID:12377789</ref> is a 27 kDa 3C-type cysteine protease, part of the VPg, and responsible for the processing of the original polyprotein into functional viral proteins. TEV protease resembles well-known [http://en.wikipedia.org/wiki/Serine_protease serine proteases], such as [[trypsin]] and [http://proteopedia.org/wiki/index.php/Chymotrypsin chymotrypsin], except that the TEV protease utilizes the nucleophilic thiol of the active site cysteine residue, as opposed to the serine hydroxyl used in serine proteases. Ultimately, the biological importance of the TEV protease requires that the enzyme have very stringent sequence specificity to ensure proper production of viral proteins, and it is based on this stringency that the TEV protease has increasingly been used to remove affinity tags from recombinant proteins. See also [[Ashley Steere/Tobacco Etch Virus (TEV) Protease]].
The [http://en.wikipedia.org/wiki/Tobacco_etch_virus tobacco etch virus] (TEV) is a member of the ''Potyviridae'' family of positive-strand RNA viruses <ref>PMID:9129643</ref>. Unlike its name suggests, TEV can infect not only the tobacco plant, but a number of other plants including some flowers (baby's breath and zinnias) and fruits/vegetables (eggplant, peppers, and tomatoes). The 5' end of the RNA genome is covalently linked to a 49 kDa virus-encoded protein or VPg (viral protein genome-linked).The positive sense genome is translated by the host's ribosomes into a large polyprotein precursor that is cleaved soon after translation to form independent protein products <ref>PMID:2254747</ref>. The TEV nuclear inclusion a (NIa) protease (shown to the right co-crystallized with peptide product in <font color='purple'><b>purple</b></font>, with the catalytic residues highlighted as <span style="color:green;background-color:black;font-weight:bold;">green</span> ball and stick models) <ref name="Phan">PMID:12377789</ref> is a 27 kDa 3C-type cysteine protease, part of the VPg, and responsible for the processing of the original polyprotein into functional viral proteins. TEV protease resembles well-known [http://en.wikipedia.org/wiki/Serine_protease serine proteases], such as [[trypsin]] and [http://proteopedia.org/wiki/index.php/Chymotrypsin chymotrypsin], except that the TEV protease utilizes the nucleophilic thiol of the active site cysteine residue, as opposed to the serine hydroxyl used in serine proteases. Ultimately, the biological importance of the TEV protease requires that the enzyme have very stringent sequence specificity to ensure proper production of viral proteins, and it is based on this stringency that the TEV protease has increasingly been used to remove affinity tags from recombinant proteins. See also [[Ashley Steere/Tobacco Etch Virus (TEV) Protease]].

Revision as of 20:50, 17 September 2014

Dimer of monomeric TEV protease catalytic domain (grey and green) complex with polypeptide substrate (pink and yellow) and acetate, 1lvm

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