This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3ju0
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | [[ | + | ==Structure of the arm-type binding domain of HAI7 integrase== |
| + | <StructureSection load='3ju0' size='340' side='right' caption='[[3ju0]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3ju0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pectobacterium_atrosepticum Pectobacterium atrosepticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JU0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3jtz|3jtz]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">intB, ECA1679 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29471 Pectobacterium atrosepticum])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ju0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ju0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ju0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ju0 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ju/3ju0_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The structures of the N-terminal domains of two integrases of closely related but not identical asn tDNA-associated genomic islands, Yersinia HPI (high pathogenicity island; encoding siderophore yersiniabactin biosynthesis and transport) and an Erwinia carotovora genomic island with yet unknown function, HAI7, have been resolved. Both integrases utilize a novel four-stranded beta-sheet DNA-binding motif, in contrast to the known proteins that bind their DNA targets by means of three-stranded beta-sheets. Moreover, the beta-sheets in Int(HPI) and Int(HAI7) are longer than those in other integrases, and the structured helical N terminus is positioned perpendicularly to the large C-terminal helix. These differences strongly support the proposal that the integrases of the genomic islands make up a distinct evolutionary branch of the site-specific recombinases that utilize a unique DNA-binding mechanism. | ||
| - | + | Structures of the arm-type binding domains of HPI and HAI7 integrases.,Szwagierczak A, Antonenka U, Popowicz GM, Sitar T, Holak TA, Rakin A J Biol Chem. 2009 Nov 13;284(46):31664-71. Epub 2009 Sep 8. PMID:19737930<ref>PMID:19737930</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
==See Also== | ==See Also== | ||
*[[Phage integrase|Phage integrase]] | *[[Phage integrase|Phage integrase]] | ||
| - | + | *[[Resolvase|Resolvase]] | |
| - | == | + | == References == |
| - | < | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Pectobacterium atrosepticum]] | [[Category: Pectobacterium atrosepticum]] | ||
| - | [[Category: Antonenka, U | + | [[Category: Antonenka, U]] |
| - | [[Category: Holak, T A | + | [[Category: Holak, T A]] |
| - | [[Category: Popowicz, G M | + | [[Category: Popowicz, G M]] |
| - | [[Category: Rakin, A | + | [[Category: Rakin, A]] |
| - | [[Category: Sitar, T | + | [[Category: Sitar, T]] |
| - | [[Category: Szwagierczak, A | + | [[Category: Szwagierczak, A]] |
[[Category: Dna binding protein]] | [[Category: Dna binding protein]] | ||
[[Category: Four stranded beta-sheet]] | [[Category: Four stranded beta-sheet]] | ||
Revision as of 08:41, 8 December 2014
Structure of the arm-type binding domain of HAI7 integrase
| |||||||||||
