3j3x
From Proteopedia
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| - | + | ==Independent reconstruction of Mm-cpn cryo-EM density map from half dataset in the closed state (training map)== | |
| - | + | <StructureSection load='3j3x' size='340' side='right' caption='[[3j3x]], [[Resolution|resolution]] 4.30Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3j3x]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanococcus_maripaludis Methanococcus maripaludis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J3X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J3X FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j3x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j3x RCSB], [http://www.ebi.ac.uk/pdbsum/3j3x PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | An increasing number of cryo-electron microscopy (cryo-EM) density maps are being generated with suitable resolution to trace the protein backbone and guide sidechain placement. Generating and evaluating atomic models based on such maps would be greatly facilitated by independent validation metrics for assessing the fit of the models to the data. We describe such a metric based on the fit of atomic models with independent test maps from single particle reconstructions not used in model refinement. The metric provides a means to determine the proper balance between the fit to the density and model energy and stereochemistry during refinement, and is likely to be useful in determining values of model building and refinement metaparameters quite generally. | ||
| - | + | Cryo-EM model validation using independent map reconstructions.,Dimaio F, Zhang J, Chiu W, Baker D Protein Sci. 2013 Jun;22(6):865-8. doi: 10.1002/pro.2267. PMID:23592445<ref>PMID:23592445</ref> | |
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| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | |||
| + | ==See Also== | ||
| + | *[[Chaperonin|Chaperonin]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Methanococcus maripaludis]] | [[Category: Methanococcus maripaludis]] | ||
| - | [[Category: Baker, D | + | [[Category: Baker, D]] |
| - | [[Category: Chiu, W | + | [[Category: Chiu, W]] |
| - | [[Category: DiMaio, F | + | [[Category: DiMaio, F]] |
| - | [[Category: Zhang, J | + | [[Category: Zhang, J]] |
[[Category: Chaperone]] | [[Category: Chaperone]] | ||
[[Category: Cryo-em model validation]] | [[Category: Cryo-em model validation]] | ||
[[Category: Independent reconstruction]] | [[Category: Independent reconstruction]] | ||
[[Category: Modeling]] | [[Category: Modeling]] | ||
Revision as of 16:39, 18 December 2014
Independent reconstruction of Mm-cpn cryo-EM density map from half dataset in the closed state (training map)
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