3zq0
From Proteopedia
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| - | + | ==Visualizing GroEL-ES in the Act of Encapsulating a Non-Native Substrate Protein== | |
| - | + | <StructureSection load='3zq0' size='340' side='right' caption='[[3zq0]], [[Resolution|resolution]] 9.20Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3zq0]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_bl21 Escherichia coli bl21] and [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZQ0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zpz|3zpz]], [[3zq1|3zq1]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zq0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zq0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zq0 RCSB], [http://www.ebi.ac.uk/pdbsum/3zq0 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The GroEL/ES chaperonin system is required for the assisted folding of many proteins. How these substrate proteins are encapsulated within the GroEL-GroES cavity is poorly understood. Using symmetry-free, single-particle cryo-electron microscopy, we have characterized a chemically modified mutant of GroEL (EL43Py) that is trapped at a normally transient stage of substrate protein encapsulation. We show that the symmetric pattern of the GroEL subunits is broken as the GroEL cis-ring apical domains reorient to accommodate the simultaneous binding of GroES and an incompletely folded substrate protein (RuBisCO). The collapsed RuBisCO folding intermediate binds to the lower segment of two apical domains, as well as to the normally unstructured GroEL C-terminal tails. A comparative structural analysis suggests that the allosteric transitions leading to substrate protein release and folding involve concerted shifts of GroES and the GroEL apical domains and C-terminal tails. | ||
| - | + | Visualizing GroEL/ES in the Act of Encapsulating a Folding Protein.,Chen DH, Madan D, Weaver J, Lin Z, Schroder GF, Chiu W, Rye HS Cell. 2013 Jun 6;153(6):1354-65. doi: 10.1016/j.cell.2013.04.052. PMID:23746846<ref>PMID:23746846</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | ==See Also== |
| - | + | *[[Chaperonin|Chaperonin]] | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Escherichia coli bl21]] | [[Category: Escherichia coli bl21]] | ||
[[Category: Escherichia coli k-12]] | [[Category: Escherichia coli k-12]] | ||
| - | [[Category: Chen, D H | + | [[Category: Chen, D H]] |
| - | [[Category: Chiu, W | + | [[Category: Chiu, W]] |
| - | [[Category: Lin, Z | + | [[Category: Lin, Z]] |
| - | [[Category: Madan, D | + | [[Category: Madan, D]] |
| - | [[Category: Rye, H S | + | [[Category: Rye, H S]] |
| - | [[Category: Schroder, G F | + | [[Category: Schroder, G F]] |
| - | [[Category: Weaver, J | + | [[Category: Weaver, J]] |
[[Category: Encapsulation]] | [[Category: Encapsulation]] | ||
[[Category: Heterogeneity]] | [[Category: Heterogeneity]] | ||
[[Category: Protein folding]] | [[Category: Protein folding]] | ||
Revision as of 09:15, 21 December 2014
Visualizing GroEL-ES in the Act of Encapsulating a Non-Native Substrate Protein
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