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3ur8
From Proteopedia
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| - | [[ | + | ==Lower-density crystal structure of potato endo-1,3-beta-glucanase== |
| + | <StructureSection load='3ur8' size='340' side='right' caption='[[3ur8]], [[Resolution|resolution]] 1.26Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3ur8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UR8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UR8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ghs|1ghs]], [[2cyg|2cyg]], [[3em5|3em5]], [[3f55|3f55]], [[3ur7|3ur7]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gluB20-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4113 Solanum tuberosum])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_endo-1,3-beta-D-glucosidase Glucan endo-1,3-beta-D-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.39 3.2.1.39] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ur8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ur8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ur8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ur8 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Endo-1,3-beta-glucanases are widely distributed among bacteria, fungi and higher plants. They are responsible for hydrolysis of the glycosidic bond in specific polysaccharides with tracts of unsubstituted beta-1,3-linked glucosyl residues. The plant enzymes belong to glycoside hydrolase family 17 (GH17) and are also members of class 2 of pathogenesis-related (PR) proteins. X-ray diffraction data were collected to 1.40 and 1.26 A resolution from two crystals of endo-1,3-beta-glucanase from Solanum tuberosum (potato, cultivar Desiree) which, despite having a similar packing framework, represented two separate crystal forms. In particular, they differed in the Matthews coefficient and are consequently referred to as higher density (HD; 1.40 A resolution) and lower density (LD; 1.26 A resolution) forms. The general fold of the protein resembles that of other known plant endo-1,3-beta-glucanases and is defined by a (beta/alpha)(8)-barrel with an additional subdomain built around the C-terminal half of the barrel. The structures revealed high flexibility of the subdomain, which forms part of the catalytic cleft. Comparison with structures of other GH17 endo-1,3-beta-glucanases revealed differences in the arrangement of the secondary-structure elements in this region, which can be correlated with sequence variability and may suggest distinct substrate-binding patterns. The crystal structures revealed an unusual packing mode, clearly visible in the LD structure, caused by the presence of the C-terminal His(6) tag, which extends from the compact fold of the enzyme molecule and docks in the catalytic cleft of a neighbouring molecule. In this way, an infinite chain of His-tag-linked protein molecules is formed along the c direction. | ||
| - | + | Two high-resolution structures of potato endo-1,3-beta-glucanase reveal subdomain flexibility with implications for substrate binding.,Wojtkowiak A, Witek K, Hennig J, Jaskolski M Acta Crystallogr D Biol Crystallogr. 2012 Jun;68(Pt 6):713-23. doi:, 10.1107/S090744491200995X. Epub 2012 May 17. PMID:22683794<ref>PMID:22683794</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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| - | + | ||
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| - | + | ||
==See Also== | ==See Also== | ||
| - | *[[ | + | *[[Glucanase|Glucanase]] |
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Glucan endo-1,3-beta-D-glucosidase]] | [[Category: Glucan endo-1,3-beta-D-glucosidase]] | ||
[[Category: Solanum tuberosum]] | [[Category: Solanum tuberosum]] | ||
| - | [[Category: Hennig, J | + | [[Category: Hennig, J]] |
| - | [[Category: Jaskolski, M | + | [[Category: Jaskolski, M]] |
| - | [[Category: Witek, K | + | [[Category: Witek, K]] |
| - | [[Category: Wojtkowiak, A | + | [[Category: Wojtkowiak, A]] |
[[Category: Carbohydrate/sugar binding]] | [[Category: Carbohydrate/sugar binding]] | ||
[[Category: Gh17 family]] | [[Category: Gh17 family]] | ||
Revision as of 09:47, 4 January 2015
Lower-density crystal structure of potato endo-1,3-beta-glucanase
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